Adenomatoid odontogenic tumor (AOT) is a rare odontogenic tumor which is often misdiagnosed as odontogenic cyst. To acquire additional information about AOT, all reports regarding AOT and cited in "pubmed" since 1990 onward were reviewed. AOT accounts for about 1% until 9% of all odontogenic tumors. It is predominantly found in young and female patients, located more often in the maxilla in most cases associated with an uneruppted permanent tooth. For radiological diagnose the intraoral periapical radiograph seems to be more useful than panoramic. However, AOT frequently resemble other odontogenic lesions such as dentigerous cysts or ameloblastoma. Immunohistochemically AOT is characterized by positive reactions with certain cytokeratins. Treatment is conservative and the prognosis is excellent. For illustration a rare case of an AOT in the mandible is presented

André C Zimmermann

Jörg GK Handschel

Norbert R Kübler

Rita A Depprich

Stefan Braunstein

PubMed

Springer - Publisher Connector

BioMed CentralHead & Face Medicine
ssOpen AcceReview
Adenomatoid odontogenic tumor of the mandible: review of the 
literature and report of a rare case
Jörg GK Handschel*1, Rita A Depprich1, André C Zimmermann1, 
Stefan Braunstein2 and Norbert R Kübler1
Address: 1Department for Cranio- and Maxillofacial Surgery, Heinrich-Heine-University, Moorenstr. 5, D-40225 Düsseldorf, Germany and 
2Department for Pathology, Heinrich-Heine-University, Moorenstr. 5, D-40225 Düsseldorf, Germany
Email: Jörg GK Handschel* - handschel@med.uni-duesseldorf.de; Rita A Depprich - depprich@med.uni-duesseldorf.de; 
André C Zimmermann - depprich@med.uni-duesseldorf.de; Stefan Braunstein - depprich@med.uni-duesseldorf.de; 
Norbert R Kübler - depprich@med.uni-duesseldorf.de
* Corresponding author    
adenomatoid odontogenic tumorreview
Abstract
Adenomatoid odontogenic tumor (AOT) is a rare odontogenic tumor which is often misdiagnosed
as odontogenic cyst. To acquire additional information about AOT, all reports regarding AOT and
cited in "pubmed" since 1990 onward were reviewed. AOT accounts for about 1% until 9% of all
odontogenic tumors. It is predominantly found in young and female patients, located more often in
the maxilla in most cases associated with an uneruppted permanent tooth. For radiological
diagnose the intraoral periapical radiograph seems to be more useful than panoramic. However,
AOT frequently resemble other odontogenic lesions such as dentigerous cysts or ameloblastoma.
Immunohistochemically AOT is characterized by positive reactions with certain cytokeratins.
Treatment is conservative and the prognosis is excellent. For illustration a rare case of an AOT in
the mandible is presented.
Adenomatoid odontogenic tumor (AOT) is a relatively
uncommon distinct odontogenic neoplasm that was first
described by Steensland in 1905 [1]. However, a variety of
terms have been used to describe this tumor. Unal et al [2]
produced a list containing all nomenclatures for AOT
reported in the literatures. Many different names like ade-
noameloblastoma, ameloblastic adenomatoid tumor,
adamantinoma, epithelioma adamantinum or teratoma-
tous odontoma have been used before to define the lesion
currently called AOT. In 1999 Philipsen and Reichart [3]
presented a review based on reports published until 1997
which showed some interesting aspects regarding epide-
miological figures of this tumor. Since then numerous
case reports of AOT have been published.
Epidemiology
From the early 1990s onwards 65 single cases of AOT
(excluding case series of more than 10 cases) have been
published. The mean age was 13.2 years (range 3 until 28
years) and the female:male ratio was 2.3 : 1. The AOT was
predominantly found in the upper jaw (maxilla:mandible
= 2.6 : 1). Regarding the various case series published in
the literature [e.g. [4-8]] and comparing these data with
the single case reports mentioned above, it has to be
Published: 24 August 2005
Head & Face Medicine 2005, 1:3 doi:10.1186/1746-160X-1-3
Received: 25 March 2005
Accepted: 24 August 2005
This article is available from: http://www.head-face-med.com/content/1/1/3
© 2005 Handschel et al; licensee BioMed Central Ltd. 
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), 
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Page 1 of 5
(page number not for citation purposes)
Head & Face Medicine 2005, 1:3 http://www.head-face-med.com/content/1/1/3reasoned that the AOT has a prevalence of odontogenic
tumors between 1.2% in caucasian [5] and 9% in black
african patients [4]. The tumor is most often diagnosed in
the second decade of life and women are about twice as
many affected than men. The AOT is over two times more
located in the maxilla than in the mandible and the ante-
rior jaw is much more affected than the posterior area.
According to Philipsen and Reichart [3] the AOT appears
in three clinico-topographic variants: follicular, extrafol-
licular and peripheral. The follicular and extrafollicular
variants are both intrabony and account for approxi-
mately 96% of all AOTs of which 71% are of follicular
type.
Clinical features
Clinical features generally focus on complaints regarding
a missing tooth. The lesion usually present as asympto-
matic swelling which is slowly growing and often associ-
ated with an unerupted tooth. However, the rare
peripheral variant occurs primarily in the gingival tissue of
tooth-bearing areas [9]. Unerupted permanent canine are
the theeth most often involved in AOTs.
Radiographic features
The radiographic findings of AOT frequently resemble
other odontogenic lesions such as dentigerous cysts, calci-
fying odontogenic cysts, calcifying odontogenic tumors,
globule-maxillary cysts, ameloblastomas, odontogenic
keratocysts and periapical disease [10]. Whereas the follic-
ular variant shows a well-circumscribed unilocular radi-
olucency associated with the crown and often part of the
root of an unerupted tooth, the radiolucency of the extra-
follicular type is located between, above or superimposed
upon the roots of erupted permanent teeth [3]. Displace-
ment of neighbouring teeth due to tumor expansion is
much more common than root resorptions. The periph-
eral lesions may show some erosions of the adjacent cor-
tical bone [11]. Comparing diagnostic arruracy between
intraoral periapical and panoramic radiographs Dare et al.
[12] found that intraoral periapical radiographs allow per-
ception of the radiopacities in AOT as discrete foci having
a flocculent pattern within radiolucency even with mini-
mal calcifies deposits while panoramic often do not.
Those calcified deposits are seen in approximately 78% of
AOT [13]. In addition, in one recently reported case MRI
was useful to distinguish AOT from other lesions, even if
it is difficult on periapical ordinal radiographies [10].
Pathohistological features
Remarkably, all variants of AOT show identical histology.
The histological typing of the WHO defined the AOT as a
tumor of odontogenic epithelium with duct-like struc-
tures and with varying degrees of inductive change in the
connective tissue. The tumor may be partly cystic, and in
Panoramic radiograph before therapyFigu e 1
Panoramic radiograph before therapy. Unicystic radiolucent lesion in the lawer right jaw with a comparatively clear demarca-
tion. The tooth 43 is located on the floor of this process. There are no resorption of the root apices.Page 2 of 5
(page number not for citation purposes)
Head & Face Medicine 2005, 1:3 http://www.head-face-med.com/content/1/1/3some cases the solid lesion may be present only as masses
in the wall of a large cyst [14]. Moreover, eosinophilic,
uncalcified, amorphous material can be found and is
called "tumor droplets". Some tumor droplets show a
homogenous matrix whereas most tumor droplets reveal
electron-dense plaques [15]. Interestingly, there are a few
reports about pigmented cells in AOT. However, all of
these reported lesions did not show macroscopically visi-
ble pigmentation. Racial pigmentation probably plays an
important role in such cases [16,17].
Immunhistological features
During the last few years several studies have been pub-
lished dealing with the immunhistological properties of
AOT. Immunohistochemically, the classical AOT pheno-
type is characterized by a cytokeratin (CK) profile similar
to follicular cyst and/or oral or gingival epithelium based
on positive staining with CK5, CK17 and CK19 [18]. On
the other hand the classical AOT is negative for CK4, 10,
13 and 18. Recently, Crivelini et al. [19] detected the
expression of cytokeratin 14 in AOT and concluded that
this probably indicate its origin in the reduced dental epi-
thelium which is also positive for staining with cytokera-
tin 14 antibodies. Positive reactions for amelogenin in
limited areas in AOT are also reported as well as in amel-
oblasts and in the immature enamel matrix [20].
Interestingly, Takahashi et al. [21] observed a positive
staining for iron-binding proteins (transferring, ferritin)
and proteinase inhibitor (alpha-one-antitrypsin) in vari-
ous cells of AOT indicating their role to the pathogenesis
of AOT. Finally, Gao et al. [22] studied the expression of
bone morphogenic protein (BMP). Whereas cementifying
fibromas, dentinomas and compound odontomas dem-
onstrated a positive reaction, all AOT as well as amelob-
lastomas and calcifying epithelial odontogenic tumors
were negative.
Treatment and prognosis
Conservative surgical enucleation is the treatment modal-
ity of choice. For periodontal intrabony defects caused by
AOT guided tissue regeneration with membrane
technique is suggested after complete removal of the
tumor [23]. Recurrence of AOT is exceptionally rare. Only
three cases in Japanese patients are reported in which the
recurrence of this tumor occurred [24]. Therefore, the
prognosis is excellent.
Tumor with fibrous connective tissue capsule (*)Figure 2
Tumor with fibrous connective tissue capsule (*). Nodular 
aggregates of cells (#). Duct-like structures (→). (HE × 50)
Gland-like spaces are surrounded by cuboidal to columnar cells (→)Figure 3
Gland-like spaces are surrounded by cuboidal to columnar 
cells (→). (HE × 160)
Tumor with calcified areas (→)Figure 4
Tumor with calcified areas (→). (HE × 200)Page 3 of 5
(page number not for citation purposes)
Head & Face Medicine 2005, 1:3 http://www.head-face-med.com/content/1/1/3Case report
A 23-year-old man was referred by his general dental prac-
titioner. One year ago the dentist diagnosed a cyst with a
ectopic lower right canine tooth by an x-ray. Beside an
uneventful medical history the patient presented no con-
spicuous intraoral clinical findings except the absence of
the tooth 43. Radiologically, he showed a 3 cm unicystic
radiolucent image with a comparatively clear
demarcation. The tooth 43 was located on the floor of this
process. No resorption of the root apices was observed
(Fig. 1).
Under general anesthesia the lesion was enucleated and
afterwards filled with pelvic spongiosa. Separating the
lesion from mandibular bone caused no problems. The
postoperative course was uneventful.
After the operation, the specimen was fixed in 4 per cent
formal saline and prepared for histological examination.
Some sections were stained with haematoxylin-eosin.
Histologically, the tumor is solid and there is a cyst forma-
tion (Fig. 2). The epithelium is in the form of whorled
masses of spindle cells as well as sheets and plexiform
strands. Rings of columnar cells give rise to duct-like
appearance (Fig. 3). Calcification is sometimes seen and
may be extensive (Fig. 4).
Half a year after surgery a clinical and radiographic follow-
up examination was performed. There was no evidence of
recurrence and no apical resorption of the adjacent teeth
could be observed (Fig. 5).
With respect to the age of the patient and the localization
of the AOT in the lower jaw, the reported case is a rare
example of this tumor entity. Beyond it our case supports
the above mentioned general description of AOTs.
Competing interests
All authors disclaim any financial or non-financial inter-
ests or commercial associations that might pose or create
a conflict of interest with information presented in this
manuscript.
Authors' contributions
JH, RD and NK made substantial contribution to the con-
ception and design of the manuscript. SB and AZ carried
out the pathohistological investigations and participated
in creating this part of the manuscript.
All authors were involved in revising the manuscript criti-
cally and have given final approval of the version to be
published.
Panoramic radiograph six months after therapyFigu e 5
Panoramic radiograph six months after therapy. No root resorption could be observed.Page 4 of 5
(page number not for citation purposes)
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Adenomatoid odontogenic tumor of the mandible: review of the literature and report of a rare case

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Epithelioma adamantinum.

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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1266042

Adenomatoid odontogenic tumor of the mandible: review of the literature and report of a rare case

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