Faculty of Food Technology and Biotechnology, University of Zagreb
Abstract
In order to develop an efficient an system for waste water pretreatment, the isolation of indigenous population of microorganisms from pharmaceutical waste water was done. We obtained pure cultures of 16 yeast isolates that differed slightly in colony morphology. Ten out of 16 isolates efficiently reduced COD in pharmaceutical waste water. Initial physiological characterization failed to match the 10 yeast isolates to either Pichia anomala or Pichia ciferrii. Restriction analysis of rDNA (rDNA-RFLP) using three different restriction enzymes: HaeIII, MspI and CfoI, showed identical patterns of the isolates and Pichia anomala type strain. Separation of chromosomal DNAs of yeast isolates by the pulsed field gel electrophoresis revealed that the 10 isolates could be grouped into 6 karyotypes. Growth characteristics of the 6 isolates with distinct karyotypes were then studied in batch cultivation in pharmaceutical waste water for 80 hours.Da bi se unaprijedio djelotvoran sustav za prethodnu obradbu otpadnih voda, izolirana je nativna populacija mikroorganizama iz otpadne vode farmaceutske industrije . Dobivene su čiste kulture 16 izolata kvasca koje su se neznatno razlikovale po morfologiji kolonija. Deset od 16 izolata učinkovito je smanjilo KPK-vrijednost u otpadnoj vodi. Početnom fiziološkom karakterizacijom nije se uspjelo utvrditi jesu li 10 izolata kvasca Pichia anomala ili Pichia ciferrii. Restrikcijska analiza rDNA (rDNA-RFLP), koristeći tri različita restrikcijska enzima HaeIII, MspI i CfoI, pokazala je identičnu strukturu izolata i soja tipa P. anomala. Separacija kromosomskih DNA u izolatima kvasca gel-elektroforezom u pulsirajućem polju otkrila je da se 10 izolata može svrstati u 6 kariotipova. Proučavane su značajke rasta 6 izolata određenoga kariotipa tijekom 80 sati šaržnog uzgoja u otpadnim vodama farmaceutske industrije
