Segmentectomy and sentinel lymph node biopsy is a golden standard for early breast cancer (clinical and radiological cT1-2, c N0). Recently, superparamagnetic iron oxide (SPIO) nanoparticle tracer has been introduced enabling intraoperative tracer injection. We prospectively recorded data on tumor histology, marked lymph nodes and their final histology and patient characteristics for early breast cancer patients who underwent breast conservative surgery. At 128 female breast cancer patients underwent sentinel lymph node biopsy by SentiMag. Three patients were excluded from further analysis
because the postoperative pathology report was ductal carcinoma in situ (DCIS). The identifi cation rate was 95.2 % (119 of 125). Of the 19.2 % (24 of 125) patients with lymph node involvement, 1.6% (2 of 125) had micrometastasis, and 1 % (1 of 125) had single tumor cells within the sentinel, l at least a micrometastasis. Of 30 positive lymph nodes removed, 24 (80 %) were true sentinel nodes. The average lymph node retrieval rate was 2.3 nodes per patient. SentiMag’s performance was comparable to published data and to standard sentinel with blue patent die and/or technetium. The benefi t of easier application and logistics is a great advantage.Segmentektomija i biopsija limfnog čvora stražara zlatni su standard za kirurško liječenje ranog raka dojke (klinički i radiološki cT1-2, c N0). Čestice superparamagnetskog željeznog oksida (superparamagnetic iron oxide, SPIO) od nedavno se
koriste kao unutaroperacijski nanočestični obilježivač. Prospektivno smo bilježili demografske podatke o bolesnicama, histologiju tumora te označenih i neoznačenih limfnih čvorova. U 128 bolesnica učinjena je biopsija limfnog čvora stražara u aksili detektorom SentiMag. Tri bolesnice su isključene, jer je konačan patohistoloski nalaz bio carcinoma ductale in situ (DCIS). Limfni čvor je pronađen u 119 od 125 bolesnica (95.2%), pozitivne limfne čvorove su imale 24 (19.2%), makrometasaze 2 (1.6%) i mikrometastaze 1 bolesnica (0.08%). Od 30 pozitivnih odstranjenih limfnih čvorava, obilježenih je bilo 24 (80
%). Prosječno su po bolesnici izvađena 2.3 limfna čvora. Mogućnost otkrivanja limfnog čvora stražara SentiMagom usporediva je s dosad objavljenim podatcima i zlatnim standardom. Prednosti metode su lakše rukovanje i minimalna logistika

Gordan Tometić

Ilija Guteša

Iva Kirac

Ivan Milas

Ivan Penavić

Ivana Pavičić

Ivana Potesak

Mladen Stanec

English

Hrčak - Portal of scientific journals of Croatia

15ARTICLE Lib Oncol. 2016;44(2–3):15–19SENTIMAG SENTINEL LYMPH NODE BIOPSY IN BREAST CONSERVATIVE SURGERY – PRELIMINARY RESULTSILIJA GUTEŠA, IVANA POTESAK, IVANA PAVIČIĆ, GORDAN TOMETIĆ, IVA KIRAC, IVAN PENAVIĆ, IVAN MILAS and MLADEN STANECDepartment of Surgical Oncology, University Hospital for Tumors, University Hospital Center Sestre milosrdnice, ZagrebSummary Segmentectomy and sentinel lymph node biopsy is a golden standard for early breast cancer (clinical and radiological cT1-2, c N0). Recently, superparamagnetic iron oxide (SPIO) nanoparticle tracer has been introduced enabling intraopera-tive tracer injection. We prospectively recorded data on tumor histology, marked lymph nodes and their fi nal histology and patient characteristics for early breast cancer patients who underwent breast conservative surgery. At 128 female breast cancer patients underwent sentinel lymph node biopsy by SentiMag. Three patients were excluded from further analysis because the postoperative pathology report was ductal carcinoma in situ (DCIS). The identifi cation rate was 95.2 % (119 of 125). Of the 19.2 % (24 of 125) patients with lymph node involvement, 1.6% (2 of 125) had micrometastasis, and 1 % (1 of 125) had single tumor cells within the sentinel, l at least a micrometastasis. Of 30 positive lymph nodes removed, 24 (80 %) were true sentinel nodes. The average lymph node retrieval rate was 2.3 nodes per patient. SentiMag’s performance was compa-rable to published data and to standard sentinel with blue patent die and/or technetium. The benefi t of easier application and logistics is a great advantage. KEY WORDS: SentiMag, Sienna+®, superparamagnetic iron oxide, early breast cancerSENTIMAG BIOPSIJA LIMFNOG ČVORA STRAŽARA U KONZERVATIVNOJ KIRURGIJI DOJKE – PRELIMINARNI REZULTATISažetak  Segmentektomija i biopsija limfnog čvora stražara zlatni su standard za kirurško liječenje ranog raka dojke (klinički i radiološki cT1-2,c N0). Čestice superparamagnetskog željeznog oksida (superparamagnetic iron oxide, SPIO) od nedavno se koriste kao unutaroperacijski nanočestični obilježivač. Prospektivno smo bilježili demografske podatke o bolesnicama, hi-stologiju tumora te označenih i neoznačenih limfnih čvorova. U 128 bolesnica učinjena je biopsija limfnog čvora stražara u aksili detektorom SentiMag. Tri bolesnice su isključene, jer je konačan patohistoloski nalaz bio carcinoma ductale in situ (DCIS). Limfni čvor je pronađen u 119 od 125 bolesnica (95.2%), pozitivne limfne čvorove su imale 24 (19.2%), makrometa-saze 2 (1.6%) i mikrometastaze 1 bolesnica (0.08%). Od 30 pozitivnih odstranjenih limfnih čvorava, obilježenih je bilo 24 (80 %). Prosječno su po bolesnici izvađena 2.3 limfna čvora. Mogućnost otkrivanja limfnog čvora stražara SentiMagom uspore-diva je s dosad objavljenim podatcima i zlatnim standardom. Prednosti metode su lakše rukovanje i minimalna logistika. KLJUČNE RIJEČI: SentiMag, Sienna+®, superparamagnetski željezni oksid, rani rak dojkeINTRODUCTIONSentinel node biopsy is the standard of care for staging of early breast cancer (1). Sentinel lymph node biopsy is accepted as the fi nal surgical proce-dure in the axilla when the sentinel node (SN) is proven negative and sometime even in the pres-ence of metastases (2). Moreover, neoadjuvant Lib Oncol. 2016;44(2–3):15–1916breast cancer treatment indications require more sentinel lymph node biopsies (3). At our Institu-tion we surgically treat over 800 newly diagnosed breast cancer patients annually. Due to well orga-nized One Day clinic a signifi cant number of pa-tients is diagnosed with early breast cancer (4). Nuclear medicine division is dislocated and this proved to complicate the standard isotope based sentinel lymph node biopsy procedure. Therefore, we welcomed the emergence of superparamag-netic iron oxyde (SPIO) nanoparticle tracer in clin-ical use backed with The SentiMag Multicentre Trial (5), The Nordic SentiMag Trial (6) and The Central European SentiMag study (7). In this pa-per, we analyzed our initial experience with the method.METHODSEarly breast cancer patients are defi ned as T1-2 axillary lymph node negative tumors on imag-ing (either ultrasound or magnetic resonance imag-ing), with primary tumor confi rmed by cytology or core biopsy. Exclusion criteria were prior breast surgery or irradiation of breast and/or axillary re-gion. Patients hypersensitive to iron products or dextrate were excluded as well as patients suﬀ ering of diseases with iron overload.  Sienna+® suspen-sion (2 ml) is applied subcutaneously under nipple areola complex followed by 5 minutes of massage to the injection area. We performed the entire se-quence when patient is already under anesthesia due to potential discomfort. There is a 20 minutes Figure 2. Detection of SIOS positive node with the probe (a) and verifi cation of the extracted node (b)Figure 1. Application of Sienna+® suspension (a) and 5 minutes massage of the area (b). 17Lib Oncol. 2016;44(2–3):15–19waiting time prior to breast lesion excision. Senti-Mag device is then applied transcutaneous to hint the sentinel node location. Axillary skin incision is then made and the lymph node is tracked by the probe signals. When the sentinel node is excised and confi rmed with the probe signal, the axilla is also checked for additional lymph nodes.The high-est displayed signal was recorded for each extract-ed and confi rmed sentinel node. The breast tumor tissue and the sentinel nodes were then analyzed on Pathology in standard fashion.RESULTSHundred and twenty eight female patients were treated with breast conservative treatment consisting of segmentectomy and SPIO tracer axil-lary sentinel lymph node biopsy. Three patients were excluded from the analysis as the fi nal pa-thology confi rmed ductal carcinoma in situ (DCIS) without invasive components.Total of 294 lymph nodes were harvested, out of which 30 were posi-tive and 24 were detected by SPIO tracer (19%). On average, 2.336 nodes were extirpated. The Sen-tiMag count was 2664 on average, ranging from 140-10000.Preoperative diagnosis was made by cytolo-gy in 68.0% (87 of 128), in 11.7% (15 out of 128) by core biopsy.We compared the lymph node positivity ac-cording to localization, but the number of tumors at each localization was not suﬃ  cient for the anal-ysis. Finally, 24 out of 24 identifi ed lymph nodes were positive, to 24 SentiMag detected additional 6 patients positive on pathology).DISCUSSIONCurrent standard for sentinel lymph node bi-opsy remains technetium 99m radioisotope and patent blue die tracers. Their application requires nuclear medicine department which makes this method unavailable to great number of surgical departments dealing with early breast cancer. The logistical simplifi cation with SPIO tracer and Sen-tiMag probe was the most important reason for adopting the technique at our institution. The Sen-tiMag Multicentre Trial (5), The Nordic SentiMag Trial (6) and The Central European SentiMag (7) study designed as non-inferiority study on 150 pa-tients showed a detection rate of 97,3% (146/150) for (99m)Tc and 98% (147/150) for Sienna+® (8). The French SentiMag feasibility trial confi rmed the non-inferiority on 108 patients. Sentinel lymph node (SLN) identifi cation rate was 98.1% (93.5-99.8) for both methods, 97.2% for Sienna+® and 95.4% for (99m) Tc and blue die. A mean of 2.1 sen-tinel lymph nodes per patient was removed (9). Italian trial confi rmed these fi ndings with detec-tion rates per patient for (99m)Tc was 99% and 97,9% for SentiMag based on 193 women analyzed in the study (10). Similar study was performed in Spain on 181 patients. Detection rates were 97.8% for SentiMag and 98.3% for (99m) Tc (concordance rate 99.4%). The study also compared ex-vivo de-tection rates (97.8%, SentiMag and 98.3% (99m)Tc); concordance rate 99.4%), transcutaneous and in-traoperative detection rates (95.5% vs 97.2%, and 97.2% vs 97.8% for SentiMag and (99m)Tc; concor-dance rates > 97%) and node level detection rates Figure 3. Localization of primary breast lesion. Overlapping  lesions denote either tumors between quadrants or multiple  tumors.Table 1.SENTINEL LYMPH NODE POSITIVITY IN CORRELATION WITH TUMOR SIZE N(%) Sentinel positiveN (%)Lymph nodes harvested N (%)pT1a 4 (3,2) 0 9 (3.08)pT1b 27 (21,6) 1 (3,7) 75 (25.68)pT1c 65 (52,0) 16 (24,6) 153 (52.4)pT2 29 (23,2) 7 (24,1) 55 (18.84)All stages 125 (100) 24 (19,2) 292 (100)Lib Oncol. 2016;44(2–3):15–1918intraoperatively and ex-vivo (92.5% vs 89.3% and 91.0% vs 86.3% for SentiMag and (99m)Tc) (11).In our preliminary fi ndings, the sensitivity was comparable to published data. Due to at least one additional lymph node harvested during the procedure we were able to detected additional 6 positive lymph nodes. Nevertheless, during the in-troduction of the method we observed inconsistent probe signals in elderly (patient born in 1929 had the minimal signal for a positive node 140), we had inconsistent data for patients with high BMI, diabe-tes, vascular diseases or smokers, therefore we could not reach a valid conclusion on these biases. The BMI issue was more carefully studied than the others, since the depth sensitivity of the probe is currently inferior to radioisotope based system (12). Experimental porcine model suggested 59 nm superparamagnetic iron oxide tracer as optimal due to speed and selectivity of node uptake (13). To further increase the sensitivity the same group achieved larger number of nodes tracked with in-creased volumes of tracers and longer waiting time to incision, up to 60 minutes (14).As for the cost eﬀ ectiveness, the standard method and SentiMag are comparable, but partial axillary lymph node dissection, spares the nerves and has the same rate of lymphedema as SLNB, harvests false negative SLNBs and is less time con-suming.Despite above mentioned shortcomings of the superparamagnetic tracer detection method, a meta-analysis by MD Anderson group showed once again a no inferiority of Sienna+® mapping agent to current standard on 1683 lymph nodes from 804 patients who participated in fi ve trials. Their conclusion will bring this method closer to becoming the standard (15). CONCLUSIONBased on these data we adopted the tech-nique and have reached similar conclusions to those already published. These are our prelimi-nary experiences and the more in depth analysis of BMI, age, localization and pathology fi ndings will be conducted in near future.REFERENCES 1. Mansel RE, Fallowfi eld L, Kissin M, Goyal A, New-combe RG,Dixon JM, et al. Randomized multicenter trial of sentinel node biopsy versus standard axillary treatment in operable breast cancer: the ALMANAC trial. J Natl Cancer Inst. 2006;98(9):599-609. 2. Lyman GH, Temin S, Edge SB, Newman LA, Turner RR, Weaver DL, et al. Sentinel lymph node biopsy for patients with early-stage breast cancer: American So-ciety of Clinical Oncology clinical practice guideline update. American Society of Clinical Oncology Clini-cal Practice. J Clin Oncol. 2014;32(13):1365-83. 3. Kaufmann M, von Minckwitz  G, Mamounas EP, Ca-meron D, Carey LA, Cristofanilli M, et al. Recommen-dations from an international consensus conference on the current status and future of neoadjuvant sys-temic therapy in primary breast cancer. Ann Surg On-col. 2012 May;19(5):1508-16. 4. Ramljak V, Vielh P. One day diagnosis. Libri Oncol. 2014;42(1-3):83-5. 5. Douek M, Klaase J, Monypenny I, Kothari A, Zeich-meister K, Brown D, et al. Sentinel node biopsy using a magnetic tracer versus standard technique: the Sen-tiMAG multicentre trial. Ann Surg Oncol. 2014;21(4):1237-45. 6. Karakatsanis A, Christiansen PM, Fischer L, Hedin C, Pistioli L, Sund M, et al. The Nordic SentiMag trial: a comparison of super paramagnetic iron oxide (SPIO) nanoparticles versus Tc(99) and patent blue in the de-tection of sentinel node (SN) in patients with breast cancer and a meta-analysis of earlier studies. Breast Cancer Res Treat. 2016;157(2):281-94. 7. Thill M, Kurylcio A, Welter R, van Haasteren V, Grosse B, Berclaz G, et al. The Central-European SentiMag study: sentinel lymph node biopsy with superpara-magnetic iron oxide (SPIO) vs. radioisotope. Breast. 2014;23(2):175-9. 8. Houpeau JL, Chauvet MP, Guillemin F, Bendavid-Athias C, Charitansky H, Kramar A, et al. Sentinel lymph node identifi cation using superparamagnetic iron oxide particles versus radioisotope: The french SentiMag feasibility trial. J. Surg. Oncology. 2016;113(5):501-7. 9. Ghilli M, Carrett a E, Di Filippo F, Batt aglia C, Fustaino L, Galanou I, et al. The superparamagnetic iron oxide tracer: a valid alternative in sentinel node biopsy for breast cancer treatment. Eur J Cancer Care (Engl). 2015 Sep 14. doi: 10.1111/ecc.12385.10. Pinero-Madrona A, Torro-Richart JA, de Leon-Carillo JM, de Castro-Parga G, Navarro-Cecilia J, Dominguez-Cunchillos F, et al.Superparamagnetic iron oxide as a tracer for sentinel node biopsy in breast cancer: A comparative non-inferiority study. Eur J Surg Oncol. 2015;41(8):991-7.11. PouW JJ, Bastiaan DM, Klaase JM, Ten Haken B. Phan-tom study quantifying the depth performance of a handheld magnetometer for sentinel lymph node bi-opsy. Phys Med. 2016;32(7):926-31.12. PouW JJ, Ahmed M, Anninga B, Schuurman K, Pinder SE, Van Hemerlrijck M,et al. Comparison of three 19Lib Oncol. 2016;44(2–3):15–19magnetic nanoparticle tracers for sentinel lymph node biopsy in an in vivo porcine model. Int J Nanomedi-cine. 2015;10:1235-43.13. Ahmed M, Anninga B, Pouw JJ, Vreemann S, Peek M, Van Hemerlijeck M, et al. Optimising magnetic senti-nel lymph node biopsy in an in vivo porcine model. Nanomedicine. 2015;11(4):993-1002.14. Teshome M, Wei C, Hunt KK, Thompson A, Rodri-guez K, Mitt endorf EA.Use of a magnetic tracer for sentinel symph sode setection in sarly-stage breast cancer Patients: A meta-analysis. Ann Surg Oncol. 2016 May;23(5):1508-14.Corresponding author: Ilija Guteša, Department of Surgi-cal Oncology, University Hospital for Tumors, Univer-sity Hospital Center Sestre milosrdnice, Ilica 197, 10000 Zagreb, Croatia. e-mail: ilija.gutesa@gmail.com

Sentimag biopsija limfnog čvora stražara u konzervativnoj kirurgiji dojke – preliminarni rezultati

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15ARTICLE Lib Oncol. 2016;44(2–3):15–19SENTIMAG SENTINEL LYMPH NODE BIOPSY IN BREAST CONSERVATIVE SURGERY – PRELIMINARY RESULTSILIJA GUTEŠA, IVANA POTESAK, IVANA PAVIČIĆ, GORDAN TOMETIĆ, IVA KIRAC, IVAN PENAVIĆ, IVAN MILAS and MLADEN STANECDepartment of Surgical Oncology, University Hospital for Tumors, University Hospital Center Sestre milosrdnice, ZagrebSummary Segmentectomy and sentinel lymph node biopsy is a golden standard for early breast cancer (clinical and radiological cT1-2, c N0). Recently, superparamagnetic iron oxide (SPIO) nanoparticle tracer has been introduced enabling intraopera-tive tracer injection. We prospectively recorded data on tumor histology, marked lymph nodes and their fi nal histology and patient characteristics for early breast cancer patients who underwent breast conservative surgery. At 128 female breast cancer patients underwent sentinel lymph node biopsy by SentiMag. Three patients were excluded from further analysis because the postoperative pathology report was ductal carcinoma in situ (DCIS). The identifi cation rate was 95.2 % (119 of 125). Of the 19.2 % (24 of 125) patients with lymph node involvement, 1.6% (2 of 125) had micrometastasis, and 1 % (1 of 125) had single tumor cells within the sentinel, l at least a micrometastasis. Of 30 positive lymph nodes removed, 24 (80 %) were true sentinel nodes. The average lymph node retrieval rate was 2.3 nodes per patient. SentiMag’s performance was compa-rable to published data and to standard sentinel with blue patent die and/or technetium. The benefi t of easier application and logistics is a great advantage. KEY WORDS: SentiMag, Sienna+®, superparamagnetic iron oxide, early breast cancerSENTIMAG BIOPSIJA LIMFNOG ČVORA STRAŽARA U KONZERVATIVNOJ KIRURGIJI DOJKE – PRELIMINARNI REZULTATISažetak  Segmentektomija i biopsija limfnog čvora stražara zlatni su standard za kirurško liječenje ranog raka dojke (klinički i radiološki cT1-2,c N0). Čestice superparamagnetskog željeznog oksida (superparamagnetic iron oxide, SPIO) od nedavno se koriste kao unutaroperacijski nanočestični obilježivač. Prospektivno smo bilježili demografske podatke o bolesnicama, hi-stologiju tumora te označenih i neoznačenih limfnih čvorova. U 128 bolesnica učinjena je biopsija limfnog čvora stražara u aksili detektorom SentiMag. Tri bolesnice su isključene, jer je konačan patohistoloski nalaz bio carcinoma ductale in situ (DCIS). Limfni čvor je pronađen u 119 od 125 bolesnica (95.2%), pozitivne limfne čvorove su imale 24 (19.2%), makrometa-saze 2 (1.6%) i mikrometastaze 1 bolesnica (0.08%). Od 30 pozitivnih odstranjenih limfnih čvorava, obilježenih je bilo 24 (80 %). Prosječno su po bolesnici izvađena 2.3 limfna čvora. Mogućnost otkrivanja limfnog čvora stražara SentiMagom uspore-diva je s dosad objavljenim podatcima i zlatnim standardom. Prednosti metode su lakše rukovanje i minimalna logistika. KLJUČNE RIJEČI: SentiMag, Sienna+®, superparamagnetski željezni oksid, rani rak dojkeINTRODUCTIONSentinel node biopsy is the standard of care for staging of early breast cancer (1). Sentinel lymph node biopsy is accepted as the fi nal surgical proce-dure in the axilla when the sentinel node (SN) is proven negative and sometime even in the pres-ence of metastases (2). Moreover, neoadjuvant Lib Oncol. 2016;44(2–3):15–1916breast cancer treatment indications require more sentinel lymph node biopsies (3). At our Institu-tion we surgically treat over 800 newly diagnosed breast cancer patients annually. Due to well orga-nized One Day clinic a signifi cant number of pa-tients is diagnosed with early breast cancer (4). Nuclear medicine division is dislocated and this proved to complicate the standard isotope based sentinel lymph node biopsy procedure. Therefore, we welcomed the emergence of superparamag-netic iron oxyde (SPIO) nanoparticle tracer in clin-ical use backed with The SentiMag Multicentre Trial (5), The Nordic SentiMag Trial (6) and The Central European SentiMag study (7). In this pa-per, we analyzed our initial experience with the method.METHODSEarly breast cancer patients are defi ned as T1-2 axillary lymph node negative tumors on imag-ing (either ultrasound or magnetic resonance imag-ing), with primary tumor confi rmed by cytology or core biopsy. Exclusion criteria were prior breast surgery or irradiation of breast and/or axillary re-gion. Patients hypersensitive to iron products or dextrate were excluded as well as patients suff ering of diseases with iron overload.  Sienna+® suspen-sion (2 ml) is applied subcutaneously under nipple areola complex followed by 5 minutes of massage to the injection area. We performed the entire se-quence when patient is already under anesthesia due to potential discomfort. There is a 20 minutes Figure 2. Detection of SIOS positive node with the probe (a) and verifi cation of the extracted node (b)Figure 1. Application of Sienna+® suspension (a) and 5 minutes massage of the area (b). 17Lib Oncol. 2016;44(2–3):15–19waiting time prior to breast lesion excision. Senti-Mag device is then applied transcutaneous to hint the sentinel node location. Axillary skin incision is then made and the lymph node is tracked by the probe signals. When the sentinel node is excised and confi rmed with the probe signal, the axilla is also checked for additional lymph nodes.The high-est displayed signal was recorded for each extract-ed and confi rmed sentinel node. The breast tumor tissue and the sentinel nodes were then analyzed on Pathology in standard fashion.RESULTSHundred and twenty eight female patients were treated with breast conservative treatment consisting of segmentectomy and SPIO tracer axil-lary sentinel lymph node biopsy. Three patients were excluded from the analysis as the fi nal pa-thology confi rmed ductal carcinoma in situ (DCIS) without invasive components.Total of 294 lymph nodes were harvested, out of which 30 were posi-tive and 24 were detected by SPIO tracer (19%). On average, 2.336 nodes were extirpated. The Sen-tiMag count was 2664 on average, ranging from 140-10000.Preoperative diagnosis was made by cytolo-gy in 68.0% (87 of 128), in 11.7% (15 out of 128) by core biopsy.We compared the lymph node positivity ac-cording to localization, but the number of tumors at each localization was not suffi  cient for the anal-ysis. Finally, 24 out of 24 identifi ed lymph nodes were positive, to 24 SentiMag detected additional 6 patients positive on pathology).DISCUSSIONCurrent standard for sentinel lymph node bi-opsy remains technetium 99m radioisotope and patent blue die tracers. Their application requires nuclear medicine department which makes this method unavailable to great number of surgical departments dealing with early breast cancer. The logistical simplifi cation with SPIO tracer and Sen-tiMag probe was the most important reason for adopting the technique at our institution. The Sen-tiMag Multicentre Trial (5), The Nordic SentiMag Trial (6) and The Central European SentiMag (7) study designed as non-inferiority study on 150 pa-tients showed a detection rate of 97,3% (146/150) for (99m)Tc and 98% (147/150) for Sienna+® (8). The French SentiMag feasibility trial confi rmed the non-inferiority on 108 patients. Sentinel lymph node (SLN) identifi cation rate was 98.1% (93.5-99.8) for both methods, 97.2% for Sienna+® and 95.4% for (99m) Tc and blue die. A mean of 2.1 sen-tinel lymph nodes per patient was removed (9). Italian trial confi rmed these fi ndings with detec-tion rates per patient for (99m)Tc was 99% and 97,9% for SentiMag based on 193 women analyzed in the study (10). Similar study was performed in Spain on 181 patients. Detection rates were 97.8% for SentiMag and 98.3% for (99m) Tc (concordance rate 99.4%). The study also compared ex-vivo de-tection rates (97.8%, SentiMag and 98.3% (99m)Tc); concordance rate 99.4%), transcutaneous and in-traoperative detection rates (95.5% vs 97.2%, and 97.2% vs 97.8% for SentiMag and (99m)Tc; concor-dance rates > 97%) and node level detection rates Figure 3. Localization of primary breast lesion. Overlapping  lesions denote either tumors between quadrants or multiple  tumors.Table 1.SENTINEL LYMPH NODE POSITIVITY IN CORRELATION WITH TUMOR SIZE N(%) Sentinel positiveN (%)Lymph nodes harvested N (%)pT1a 4 (3,2) 0 9 (3.08)pT1b 27 (21,6) 1 (3,7) 75 (25.68)pT1c 65 (52,0) 16 (24,6) 153 (52.4)pT2 29 (23,2) 7 (24,1) 55 (18.84)All stages 125 (100) 24 (19,2) 292 (100)Lib Oncol. 2016;44(2–3):15–1918intraoperatively and ex-vivo (92.5% vs 89.3% and 91.0% vs 86.3% for SentiMag and (99m)Tc) (11).In our preliminary fi ndings, the sensitivity was comparable to published data. Due to at least one additional lymph node harvested during the procedure we were able to detected additional 6 positive lymph nodes. Nevertheless, during the in-troduction of the method we observed inconsistent probe signals in elderly (patient born in 1929 had the minimal signal for a positive node 140), we had inconsistent data for patients with high BMI, diabe-tes, vascular diseases or smokers, therefore we could not reach a valid conclusion on these biases. The BMI issue was more carefully studied than the others, since the depth sensitivity of the probe is currently inferior to radioisotope based system (12). Experimental porcine model suggested 59 nm superparamagnetic iron oxide tracer as optimal due to speed and selectivity of node uptake (13). To further increase the sensitivity the same group achieved larger number of nodes tracked with in-creased volumes of tracers and longer waiting time to incision, up to 60 minutes (14).As for the cost eff ectiveness, the standard method and SentiMag are comparable, but partial axillary lymph node dissection, spares the nerves and has the same rate of lymphedema as SLNB, harvests false negative SLNBs and is less time con-suming.Despite above mentioned shortcomings of the superparamagnetic tracer detection method, a meta-analysis by MD Anderson group showed once again a no inferiority of Sienna+® mapping agent to current standard on 1683 lymph nodes from 804 patients who participated in fi ve trials. Their conclusion will bring this method closer to becoming the standard (15). CONCLUSIONBased on these data we adopted the tech-nique and have reached similar conclusions to those already published. These are our prelimi-nary experiences and the more in depth analysis of BMI, age, localization and pathology fi ndings will be conducted in near future.REFERENCES 1. Mansel RE, Fallowfi eld L, Kissin M, Goyal A, New-combe RG,Dixon JM, et al. Randomized multicenter trial of sentinel node biopsy versus standard axillary treatment in operable breast cancer: the ALMANAC trial. J Natl Cancer Inst. 2006;98(9):599-609. 2. Lyman GH, Temin S, Edge SB, Newman LA, Turner RR, Weaver DL, et al. Sentinel lymph node biopsy for patients with early-stage breast cancer: American So-ciety of Clinical Oncology clinical practice guideline update. American Society of Clinical Oncology Clini-cal Practice. J Clin Oncol. 2014;32(13):1365-83. 3. Kaufmann M, von Minckwitz  G, Mamounas EP, Ca-meron D, Carey LA, Cristofanilli M, et al. Recommen-dations from an international consensus conference on the current status and future of neoadjuvant sys-temic therapy in primary breast cancer. 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Sentimag biopsija limfnog čvora stražara u konzervativnoj kirurgiji dojke – preliminarni rezultati

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