Parathion content in individual tissues of acutely poisoned rats

Abstract

Određivan je paration u mozgu, jetri i mišićnom tkivu pacova nakon jednokratne intraperitonealne aplikacije otrova. Određivanje je vršeno metodom gasno-tečne hromatografije, hromatografom firme Varian, model 2100-20, opremljenim fosfornim detektorom. Kolona je punjena smešom dve stacionarne faze (silikonsko ulje DC-200 i QF-1) na Chromosorb W-HMDS, kao nosaču. U mozgu nisu nađene merljive količine parationa. Takođe, nije nađen paration u jetri i trbušnim mišićima u toku prvih 60 minuta trovanja. U dužim vremenskim intervalima nađene količine parationa iznosile su 0,125-7,158 µg/g tkiva.Parathion determinations were carried out in brain liver and muscle tissues of the rats poisoned by a single intraperitoneal injection, using gas-liquid chromatography. »Varian« chromatograph, model 2100-20, was used, equipped with a phosphorus detector. The column was packed with a mixture of two stationary phases (silicone oil DC-200 and OF-1) on Chromosorbe W-HMDS. No measurable quantities of parathion were found in the brain. During the first 60 minutes after the poison injection there was no parathion in the liver or abdominal muscles either. In longer time intervals, the detected quantities of parathion were in the range from 0.125 to 7.158 µg/g of the tissue

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