Faculty of Veterinary Medicine University of Zagreb
Abstract
The present study describes the occurrence of dermatophytes and their antifungal susceptibility in healthy rabbits kept on a farm or in a household as companion animals. Out of 614 animals examined, 146 samples (23.78%) were positive for dermatophytes by direct microscopic examination, and the dermatophytes were isolated from 112 samples (112/146, 76.71%). The four predominant species of dermatophytes isolated from healthy rabbits were Trichophyton mentagrophytes (37.5%), Microsporum gypseum (25%), Microsporum nanum (21.43%) and Microsporum canis (16.07%). The kits younger than 6 months of age showed a relatively higher occurrence rate, and male animals showed significantly higher occurrence than the females (P<0.05). Occurrence of dermatophytes in the studied rabbits was higher in summer and autumn than in spring and winter (P<0.05). Itraconazole (0.03-0.5 μg/mL), ketoconazole (0.06-0.5 μg/mL), miconazole at 0.03-0.5 μg/mL and 0.03-.25 μg/mL concentrations showed the lowest MIC values against T. mentagrophytes, M. canis, M. nanum and M. gypseum, respectively. However, fluconazole and griseofulvin showed the highest MIC value against the isolates, indicating antifungal resistance.U radu je opisan nalaz dermatofita i njihova osjetljivost na protugljivične lijekove u zdravih kunića držanih na farmi ili u domaćinstvu kao kućni ljubimci. Od 614 pretraženih životinja na dermatofite je bilo pozitivno 146 uzoraka (23,78%) izravnom mikroskopskom pretragom, a dermatofiti su bili izdvojeni iz 112 uzoraka (112/146, 76,71%). Dominantne vrste dermatofita izdvojene iz zdravih kunića bile su Trichophyton mentagrophytes (37,5%), Microsporum gypseum (25%), Microsporum nanum (21,43%) i Microsporum canis (16,07%). Stopa pojavnosti bila je relativno viša u mladunaca do 6 mjeseci, a u mužjaka je bila značajno viša nego u ženki (P<0,05). Dermatofiti su češće bili izdvojeni ljeti i u jesen nego u proljeće i zimi (P<0,05). Najmanja minimalna inhibicijska koncentracija (MIK) itrakonazola od 0,03-0,5 μg/mL dokazana je za T. mentagrophytes, ketokonazola od 0,06-0,5 μg/mL za M. canis, mikonazola od 0,03-0,5 μg/mL za M. nanum i od 0,03-0,25 μg/mL za M. gypseum. Flukonazol i grizeofulvin pokazivali su najvišu vrijednost MIK što govori o otpornosti izolata na te lijekove
