Division of Biology, Faculty of Science, University of Zagreb
Abstract
In eight embryogenie tissue lines of omorika spruce the roles of carbohydrates and abscisic acid on embryo maturation were examined. Sucrose, glucose and maltose (3% and 6%) with abscisic acid (20, 40, 60 μM) were screened for optimal combination with respect to embryo maturation. The B lines, which expressed a brown tissue phenotype, achieved superior embryo maturation on 3% glucose and 40 μM abscisic acid. The VV lines with a white and translucent tissue phenotype showed the best maturation capacity on 3% sucrose or 3% maltose with 20 or 40 μM ABA. Sixty μM ABA inhibited embryo maturation. Media containing 6 % of either sucrose, glucose and maltose produced fewer mature embryos compared to media with 3% of the same sugars. The VV lines had almost three times lower maturation capacity in relation to the B lines
