Division of Biology, Faculty of Science, University of Zagreb
Abstract
A virus (SMV-J) in Chenopodium murale collected near Nova Gra- diska (Yugoslavia) was found to be an isolate of sowbane mosaic virus (SMV). The virus was transmitted to 8 species of Chenopodiaceae but it failed to infect 7 species of plants from other families. The virus was isolated and purified. Electron microscope analyses revealed isometric virus particles about 30 nm in size. SMV-J was transmitted readily through seed; 32% of the seeds of diseased plants produced infected seedlings.
The virus has a thermal inactivation point at about 93°C. It remained active in vitro more than 2 months. An immune serum against SMV-J was prepared. The serological tests performed by the agar gel double diffusion test and the intragel absorption test showed that SMV-J is indistinguishable from the American strain of SMV. In addition, SMV-J did not differ serologically from SMV isolate found in grapevine in YugoslaviaNa primjercima vrste Chenopodium murale koji su sabrani u okolici N. Gradiške nađen je virus (SMV-J), koji predstavlja izolat virusa mozaika lobode (sowbane mosaic virus, SMV). Virus je prenijet na osam vrsta iz porodice Chenopodiaceae, ali ga nismo uspjeli prenijeti na sedam vrsta koje pripadaju drugim biljnim porodicama. Virus je izoliran i purificiran prema metodi Steere (1959). Elektronskomikroskopskom analizom purificirane virusne suspenzije nađene su izometrične virusne čestice promjera oko 30 nm. SMV-J lako se prenosi sjemenom vrste C. murale. Iz 32°/o sjemenaka, sabranih u polju na inficiranoj biljci, nikle su zaražene biljke. Termalna točka inaktivacije istraživanog virusa je oko 93 °C, a može postojati in vitro više od 2 mjeseca. Krajnja točka razrjeđenja mu je oko 10-7.
Protiv SMV-J priređen je imuni serum. Serološki pokusi izvedeni metodom dvostruke difuzije u agarskom gelu te metodom unakrsnog zasićavanja u agaru pokazali su da se SMV-J ne razlikuje od američkog soja virusa mozaika lobode (SMV-A). On se od SMV-A ne razlikuje niti po elektroforetskoj pokretljivosti. Osim toga SMV-J se serološki ne razlikuje niti od izolata SMV koji je nađen u vinovoj lozi u Jugoslaviji
