Guernsey, L

Han, D

Secor, E

Silbart, L

Szczepanek, S

Thrall, R

English

PubMed

E Secor

S Szczepanek

L Guernsey

L Silbart

R Thrall

D Han

Springer - Publisher Connector

POSTER PRESENTATION Open Access
P01.22. Identification of a peptide biomarker from
bromelain, an extract of ananas comosus merr,
using LC-SRM/MS
E Secor1*, S Szczepanek2, L Guernsey1, L Silbart3, R Thrall1, D Han4
From International Research Congress on Integrative Medicine and Health 2012
Portland, Oregon, USA. 15-18 May 2012
Purpose
Bromelain, a pineapple extract, is a complex mixture of
proteases and protease inhibitors. Although bromelain
(Br) has been used clinically for over 40 years, the active
constituents that mediate its anti-inflammatory activity
are not thoroughly characterized and no biomarker
exists to evaluate absorbed peptides and their potential
therapeutic responses.
Methods
Stem Br (Vital Nutrients lot # 2890; Middletown, CT),
was tested for authenticity and purity. Female C57BL/6J
mice, (Jackson Laboratory; Bar Harbor, ME) received
one bolus, i.p. injection of Br (12mg/kg) in 0.5ml of phy-
siological saline and plasma collected at baseline 3, 6, 12
and 24 hours. All procedures were approved by the Ani-
mal Care Committee at University of CT Health Center.
Post SDS-PAGE in gel digestion, samples (Br raw mate-
rial, spiked plasma and plasma from i.p. teated animals)
were analyzed via LC/MS/MS (Liquid Chromatography-
Mass Spectrometry-Mass) and LC-SRM/MS (Liquid
Chormatography-Selected Reaction Monitoring/Mass
Spectrometry). Data were searched using Mascot and
Scaffold algorithms.
Results
Within the Br raw material we identified 44 proteins
of which several were Br-specific including nine pro-
teases, one glycosidase and three protease inhibitors.
In Br spiked plasma, 7 Br-specific proteins (ananain,
Br inhibitor, comosain, cysteine proteinase precursor
ANll, FB1035 precursor, FBSB precursor and jacalin-
like lectin), similar to those found in the Br raw mate-
rial, were identified. Within these 7 proteins, 21 Br-
specific peptides were further identified and charac-
terized based on their ion spectrum and fragmenta-
tion patterns including the unique peptide,
DYGAVNEVK. Using SRM DYGAVNEVK was identi-
fied in plasma of Br-treated mice. The spectral count
of DYGAVNEVK peaked at 6 hours and was unde-
tectable by 24 hours.
Conclusion
This Br peptide could serve as a biomarker to standar-
dize the therapeutic dose or Br and maximize its clinical
utility.
Author details
1University of Connecticut Health Center, Farmington, USA. 2Neag Cancer
Center, UCHC, Farmington, USA. 3Department of Allied Health Sciences,
Storrs, USA. 4Center for Vascular Biology, UCHC, Farmington, USA.
Published: 12 June 2012
doi:10.1186/1472-6882-12-S1-P22
Cite this article as: Secor et al.: P01.22. Identification of a peptide
biomarker from bromelain, an extract of ananas comosus merr, using
LC-SRM/MS. BMC Complementary and Alternative Medicine 2012 12(Suppl
1):P22.
1University of Connecticut Health Center, Farmington, USA
Full list of author information is available at the end of the article
Secor et al. BMC Complementary and Alternative Medicine 2012, 12(Suppl 1):P22
http://www.biomedcentral.com/1472-6882/12/S1/P22
© 2012 Secor et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


P01.22. Identification of a peptide biomarker from bromelain, an extract of ananas comosus merr, using LC-SRM/MS

Crossref

BMC Complementary and Alternative Medicine

Secor E

Szczepanek S

Guernsey L

Silbart L

Thrall R

Han D

Directory of Open Access Journals

file:///data/core-remote/dit/data/Springer-OA/pdf/074/aHR0cDovL2xpbmsuc3ByaW5nZXIuY29tLzEwLjExODYvMTQ3Mi02ODgyLTEyLVMxLVAyMi5wZGY=.pdf

P01.22. Identification of a peptide biomarker from bromelain, an extract of ananas comosus merr, using LC-SRM/MS

Abstract

Similar works

Full text

Available Versions

Springer - Publisher Connector

Crossref

Directory of Open Access Journals