Friebe, Andreas

Groneberg, Dieter

Jäger, Ronald

BMC Pharmacology

English

PubMed

Ronald Jäger

Dieter Groneberg

Andreas Friebe

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Role of NO/cGMP signalling in VEGF-mediated
angiogenesis
Ronald Jäger*, Dieter Groneberg, Andreas Friebe
From 5th International Conference on cGMP: Generators, Effectors and Therapeutic Implications
Halle, Germany. 24-26 June 2011
Background
NO-sensitive guanylyl cyclase (NO-GC) is the most
important effector protein for nitric oxide (NO) and is
involved in various physiological processes. Binding of
NO stimulates NO-GC up to 200-fold and thereby
increases cGMP synthesis. NO is generated by different
NO synthases (NOS). In the vasculature, endothelial
NOS (eNOS) is involved in the regulation of blood pres-
sure by relaxation of vascular smooth muscle. eNOS is
known to be stimulated by shear stress. In addition, NO
synthesis is stimulated by vascular endothelial growth
factor (VEGF) which acts as one of the most potent sti-
mulators of angiogenesis.
Until now the exact signalling pathway of VEGF-
induced NO has not been elucidated. S-nitrosylation of
protein thiols as well as stimulation of NO-GC with
subsequent cGMP increases may underlie the angiogenic
effect of VEGF.
Method
In order to investigate the involvement of the NO/cGMP
cascade in VEGF-mediated angiogenesis we generated an
endothelial-specific NO-GC knock-out mouse (EC-
GCKO) using the Tie2-Cre line.
Results
Preliminary macroscopic analysis revealed that EC-
GCKO mice are indistinguishable from WT animals and
do not show any of the gross phenotypes of the total
GCKO mice. Several in vitro and in vivo techniques are
being used such as primary endothelial cell culture, aor-
tic ring assay and matrigel plug analysis.
Published: 1 August 2011
doi:10.1186/1471-2210-11-S1-P35
Cite this article as: Jäger et al.: Role of NO/cGMP signalling in VEGF-
mediated angiogenesis. BMC Pharmacology 2011 11(Suppl 1):P35.
Submit your next manuscript to BioMed Central
and take full advantage of: 
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
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* Correspondence: ronald.jaeger@uni-wuerzburg.de
Physiologisches Institut, Universität Würzburg
Jäger et al. BMC Pharmacology 2011, 11(Suppl 1):P35
http://www.biomedcentral.com/1471-2210/11/S1/P35
© 2011 Jäger et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


Role of NO/cGMP signalling in VEGF-mediated angiogenesis

Crossref

POSTER PRESENTATION Open AccessRole of NO/cGMP signalling in VEGF-mediatedangiogenesisRonald Jäger*, Dieter Groneberg, Andreas FriebeFrom 5th International Conference on cGMP: Generators, Effectors and Therapeutic ImplicationsHalle, Germany. 24-26 June 2011BackgroundNO-sensitive guanylyl cyclase (NO-GC) is the mostimportant effector protein for nitric oxide (NO) and isinvolved in various physiological processes. Binding ofNO stimulates NO-GC up to 200-fold and therebyincreases cGMP synthesis. NO is generated by differentNO synthases (NOS). In the vasculature, endothelialNOS (eNOS) is involved in the regulation of blood pres-sure by relaxation of vascular smooth muscle. eNOS isknown to be stimulated by shear stress. In addition, NOsynthesis is stimulated by vascular endothelial growthfactor (VEGF) which acts as one of the most potent sti-mulators of angiogenesis.Until now the exact signalling pathway of VEGF-induced NO has not been elucidated. S-nitrosylation ofprotein thiols as well as stimulation of NO-GC withsubsequent cGMP increases may underlie the angiogeniceffect of VEGF.MethodIn order to investigate the involvement of the NO/cGMPcascade in VEGF-mediated angiogenesis we generated anendothelial-specific NO-GC knock-out mouse (EC-GCKO) using the Tie2-Cre line.ResultsPreliminary macroscopic analysis revealed that EC-GCKO mice are indistinguishable from WT animals anddo not show any of the gross phenotypes of the totalGCKO mice. Several in vitro and in vivo techniques arebeing used such as primary endothelial cell culture, aor-tic ring assay and matrigel plug analysis.Published: 1 August 2011doi:10.1186/1471-2210-11-S1-P35Cite this article as: Jäger et al.: Role of NO/cGMP signalling in VEGF-mediated angiogenesis. BMC Pharmacology 2011 11(Suppl 1):P35.Submit your next manuscript to BioMed Centraland take full advantage of: • Convenient online submission• Thorough peer review• No space constraints or color figure charges• Immediate publication on acceptance• Inclusion in PubMed, CAS, Scopus and Google Scholar• Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submit* Correspondence: ronald.jaeger@uni-wuerzburg.dePhysiologisches Institut, Universität WürzburgJäger et al. BMC Pharmacology 2011, 11(Suppl 1):P35http://www.biomedcentral.com/1471-2210/11/S1/P35© 2011 Jäger et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.

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Role of NO/cGMP signalling in VEGF-mediated angiogenesis

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