The authors present the research results of the influence of aqueous solution of calcium pantothenate, nicotinamide, cyanocobalamin, folacin and L-carnitine influence on the dynamics of morphological and biochemical blood indices of broiler-chickens in the condition of industrial technology of chicken raisin

Khmyrov, A. V.

Shaposhnikov, A. A.

Sidorenko, L. L.

Zakirova, L. R.

DSpace at Belgorod State University

51Сетевой научно-практический журналс е р и я  Медицина и фармацияТ Т А У Ч Н Ы ЙРЕЗУЛЬТАТUDC 636.5.085.16: 577.164: 591.436Shaposhnikov A A ., Khmyrov A.V., Zakirova L.R., Sidorenko L.L.BLOOD INDICES OF BROILER-CHICKENS AFTER SUPPLEMENTING THEIR DIET WITH SOLUTIONS CONTAINING B-GROUP AND L-CARNITINE VITAMINSS h a p o sh n ik o v  A n d r e y  A le x a n d ro v ic h , D octor o f  B io log ical Sciences, P rofessor  Head of Department of Biochemistry Belgorod State National Research University 85 Pobedy St., Belgorod, 308015, Russia; E-mail: shaposhnikov@bsu.edu.ru; phone:+7(4722)30-14-11 K h m y ro v  A le k s e y  V la d im ir o v ic h , PhD  in  B io log ical Sciences  Head of the Veterinary Department of Belgorod Region, the Chief State Veterinary Inspector of Belgorod region; 22A 1-Michurinsky Lane, Belgorod, 308014, Russia,E-m ail: veter@ belgorod.com ; p hone: + 7 (4722) 31-27-60  Z a k ir o v a  L y u d m ila  R o b e rto v n a , PhD  in  B io log ical Sciences, A ssocia te  P rofessor  Department of Biochemistry Belgorod State National Research University 85 Pobedy St., Belgorod, 308015, Russia; E-m ail: zakirova@ bsu.edu.ru; p hone: + 7(4722)30-14-11S id o re n k o  L y u b o v  L e o n id o v n a , P ostg radu ate Studen t  Belgorod State National Research University, 85 Pobedy St., Belgorod, 308015, Russia E -m ail: sidorenko_ l@ bsu.edu.ru; p hone:+ 7(4722)30-14-11i"YBSTRAKTThe authors present the research results of the influence of aqueous solution of calcium pantothenate, nicotinamide, cyanocobalamin, folacin and L-carnitine influence on the dynamics of morphological and biochemical blood indices of broiler-chickens in the condition of industrial technology of chicken raising. I V 'e y  w o rd s: broiler-chickens; diet, dietary supplement; erythrocytes and leucocytes; blood pigment; Kblood serum protein.А. А. Шапошников, А. В. Хмыров,Л.Р. Закирова,Л. Л. СидоренкоПОКАЗАТЕЛИ КРОВИ ЦЫПЛЯТ- БРОЙЛЕРОВ ПОД ДЕЙСТВИЕМ ВВЕДЕНИЯ В ИХ ДИЕТУ РАСТВОРА ВИТАМИНОВ ГРУППЫ В И  L-КАРНИТИНАШ а п о ш н и к о в  А н д р е й  А л е к са н д р о в и ч ,докт ор биологических наук, проф ессор, заведую щ ий каф едрой биохим ии  Белгородского государственного национального исследовательского университета,308015, г. Белгород, ул. Победы, 85, E-mail: shaposhnikov@bsu.edu.ru, тел.+7(4722)зо-14-11Х м ы р о в  А л е к с е й  В л а д и м и р о в и ч , кандидат  биологических наук, начальник управления вет еринарии Б елгородской  област и, главны й государст вен н ы й вет еринарны й инспект ор Б елгородской област и,308014, г. Белгород, 1-й Мичуринский пер., 22а, E-mail: veter@belgorod.com, тел. +7 (4722) 31-27-60З а к и р о в а  Л ю д м и л а  Р о б е р то в н а , кандидат  биологических наук, доцент  каф едры  биохим ии  Белгородского государственного национального исследовательского университета,308015, г. Белгород, ул. Победы, 85, E-mail: zakirova@bsu.edu.ru, тел. +7(4722)30-14-11 С и д о р е н к о  Л ю б о в ь  Л е о н и д о в н а , аспирант  Белгородского государственного национального исследовательского университета,308015, г. Белгород, ул. Победы, 85, E-mail: sidorenko_l@bsu.edu.ru, тел.+7(4722)з0-14-11А Н Н О Т А Ц И ЯПредставлены результаты исследования влияния водного раствора кальция пантотената, ни- котинамида, цианокобаламина, фолиевой кислоты и L-карнитина на динамику морфоло­гических и биохимических показателей крови цыплят-бройлеров в условиях промышленной технологии их выращивания.Кл ю ч е в ы е  сл ова: цыплята-бройлеры, диета, биологически активная добавка, эритроциты и лейкоциты, гемоглобин, белки сыворотки крови.№1 2015Shaposhnikov A.A., Khmyrov A.V., Zakirova L.R., Sidorenko L.L.BLOOD INDICES OFBROILER-CHICKENS AFTER SUPPLEMENTINGTHEIR DIET WITH SOLUTIONS CONTAINING B-GROUP AND L-CARNITINE VITAMINSА У Ч Н Ы ИРЕЗУЛЬТАТСетевой научно-практический журналR elevan ce. In the industrial poultry farming, which is connected to its isolation from the natu­ral sources of feeding, vitamins, especially the vi­tamins of B-group, play an important role [3]. An attempt to understand the influence of vitamin and L-carnitine combination on broiler-chickens growth and evolution, the morphological and bio­chemical blood indices [1, 2, 4, 5] and on the base of this an opportunity to give a characteristics of metabolic processes in the poultry organism were taken for the first time [6, 7].T h e  p u rp o s e  o f  th e  w o rk . To fix a morpho­logical and biochemical modifications of blood characteristics of intensively growing chickens using an aqueous solution of vitamins B-group and L-carnitine fluid (commercial preparation «Vitogon», produced by LLC «Belfarmacom», Russia).M a te ria ls  a n d  m e th o d s  o f  th e  stu d y.The test was conducted on the broiler-chickens of the cross “Hubbard” in the period from 1 to 41 days of age. The experimental setup, feeding and used doses of the drug “Vigoton” in the diets of broilers are presented in Table 1.The study of morphological and biochemical parameters of the birds’ liver was determinedHematological indices o f  broiler-chiby standard techniques, using the equipment accredited in the testing laboratory at Belgorod State Agricultural University named after V. Gorin.T h e  re s u lts  o f  re s e a rc h . It was revealed, that the concentration of erythrocytes and leu­cocytes, and hemoglobin contents of all groups of birds were within the physiological standard. On the 21st day of the experiment, under the in­fluence of the drink preparation «Vitogon», the concentration of blood pigment in the second and fourth groups of chicken-broilers has be­come actually less in comparison with the birds in the control group (Table №1). These modifi­cations from the biological point of view, are not significant, don’t create problems in provision of tissues and organs with oxygen, and in eliminat­ing СО2 and hydrogen proton.In the 2nd and 3d groups of chickens, the concentration of blood pigment was actually 4.5 and 5.8 % less accordingly, in comparison with the control group. Besides, these distinctions are not significant and might be explained by un­avoidable errors which occur in the receipt pro­cess of whole blood and realization of analytical procedures.Table 1ens on the 21 and 41 days o f  growth (n=5)Index Age, daysNumber of test group1 2 3 4Hemoglobin, g/l21 91.7±1.41 87.2±0.58* 89.0±1.13 86.4±0.56*41 96.7±2.21 106.2±8.53 93.5±3.32 102.6±4.13Erythrocyte,1012/l21 2.8±0.28 2.7±0.18 2.3±0.14 2.2±0.1041 2.9±0.24 2.8±0.29 2.6±0.31 2.8±0.41Leucocyte, 109/l21 39.6±2.91 41.2±3.09 39.6±4.11 42.1±5.1841 39.8±5.21 40.1±7.11 40 .1±4.99 41.4±3.80Here and further on: *р<0.05; **р<0.01; ***р<0.001On the 41 day, the level of the blood pigment of broiler-chickens in the 2nd and 4th groups was 9.8 and 6.1% higher in comparison with the control group accordingly. Despite the big difference, the true distinctions in this case were not detected.The modification of the concentration of eryth­rocytes and leucocytes, as on the 21st and 41 days, doesn’t significantly differ in the groups. The re­search results of some characteristics of the ami­no acid metabolism are listed in Table №2. The concentration of total protein of blood serum wasmostly on the same level in all broiler-chickens groups.We should note, that using the «Vitogon» preparation has slightly increased the concentra­tion of total protein and the level of albumen. It’s well known, that serum albumin is an important source of amino acids, which is important for bio­synthesis of muscle proteins. Taking into account the experimentally proven increase of body weight gain in the 2nd and 4th groups of chickens, we may conclude that such modifications are a biochemicalСЕРИЯ Медицина и фармацияShaposhnikov A.A., Khmyrov A.V., Zakirova L.R., Sidorenko L.L.BLOOD INDICES OFBROILER-CHICKENS AFTER SUPPLEMENTINGTHEIR DIET WITH SOLUTIONS CONTAINING B-GROUP AND L-CARNITINE VITAMINS\А У Ч Н Ы ИРЕЗУЛЬТАТСетевой научно-практический журналbase of the growth and improvement of develop­ment.The discussion of obtained results regarding y-globulin is always sophisticated, as its increase may be estimated dually: as an immunity strength­ening or defense reaction on the action of different factors of the external environment (infections, in­flammatory processes etc.) Nevertheless, we rec­ognized the tendency of y-globulin decrease in the blood serum of chickens in test groups. We also in­dividually determined the concentration of Ig im­munoglobulins, which was truly lower in chickens receiving «Vitogon». Thus, we may conclude that the preparation used in the study has immune-pro­tective characteristics.Table 2The concentration o f  serum proteins in the blood o f  chickens (n=5)Index Age, daysNumber of test group1 2 3 4Total protein, g/l21 39.2±0.31 40 .8±0.49 41.1±0.31 42.0±0.7041 40.1±0.32 41.2±0.41 41.5±0.27 40 .9±0.57Fractions of total protein, %:albumen21 40.6±1.39 42.1±1.21 41.9±0.98 42.0±1.5641 41.8±1.22 41.5±1.43 42.1±1.65 42.2±1.27a-globulin21 21.1±0.84 20.4±0.29 21.9±0.34 22.6±0.6741 23.3±0.69 21.7±0.71 22.7±0.66 22.9±1.05P-globulin, %21 14.6±0.38 15.4±0.41 14.9±0.56 14.5±0.4941 14.8±0.69 17.2±0.51 15.3±0.98 13.6±0.70y-globulin21 23.7±0.99 22.1±1.03 21.3±0.67 20.9±0,7941 20.1±0.24 19.6±0.78 19.9±1.41 21.3±0.82The coefficient of albumen/globulin21 0.68 0.73 0.72 0.7241 0.72 0.71 0.73 0.73T h e c o n c lu sio n . The peroral use of calcium panteona, niacinamide, cyanocobalamin, folacin and L-carnitine («Vitogon» preparation) has apositive influence on the processes of hemato- poiesis and proteometabolism, which contribute to an intensive growth of broiler-chickens.R E FER E N CE S:1. Davis C.Y. Effect of all-trans Retinol and Reti- noic Acid Nutritive on the Immune System of Chicks / C. Y. Davis, I. L. Sell // 1. Nutr. 2003. Vol. 113. Pp.1914-1919.2. Khenning A. Mineral Substances, Vitamins, Biostimulants in Feeding of Agricultural Ani­mals. Moscow: Kolos, 2010. 558 p.3. Valdman A.R., Surai P.F., Ionov I.A., Sakhatsky N.I. Vitamins in Animal Feed (Metabolism and Requirement). -  Kharkov: publishing house: Original, 1993. 423 p.4. Vasilenko V.N., Makarova I.V. Hematologic Indices of Broiler-chickens after Using L-Car- nitin in their Diet. / V.N. Vasilenko, I.V. Ma­karova // Poultry and poultry products. 2009. №1. Pp. 45-46.5. Maslyuk, A.N. The Effect of Different Doses of Ascorbic and Nicotinic Acids on Morphofunc- tional Parameters of the Males-broilers: Ab­stract dis. .. candidate of biol. Sciences. Ekat­erinburg, 2007. 22 p.: ill.6. Bevzyuk V.N. Unconventional Feed and En­zyme Preparations in the Feed of Meat Poul­try: Abstract dis. ... Dr. agricultural Sciences. settl. Persianovsky, 2005. 47 p.7. Mordakin V.N. Economic and Biological Char­acteristics of Broiler-chickens of “Smena-4” Cross Fed on Ascorbic, Citric and Fumaric Ac­ids: Abstract dis. ... candidate of agricultural Sciences. Ryazan, 2006. 18 p.СЕРИЯ Медицина и фармация

Blood indices of broiler-chickens after supplementing their diet with solutions containing B-group and L-carnitine vitamins

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Blood indices of broiler-chickens after supplementing their diet with solutions containing B-group and L-carnitine vitamins

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