Barletta, Bianca

Barone, Andrea

Butteroni, Cinzia

Corinti, Silvia

Di Felice, Gabriella

Schiavi, Elisa

English

PubMed

Elisa Schiavi

Bianca Barletta

Andrea Barone

Cinzia Butteroni

Silvia Corinti

Gabriella Di Felice

Crossref

Clinical and Translational Allergy

Probiotic treatment induces intestinal regulatory dendritic and T cells, and counter-regulates Th2 responses and anaphylaxis in a mouse model of food allergy

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ORAL PRESENTATION Open Access
Probiotic treatment induces intestinal regulatory
dendritic and T cells, and counter-regulates Th2
responses and anaphylaxis in a mouse model of
food allergy
Elisa Schiavi*, Bianca Barletta, Andrea Barone, Cinzia Butteroni, Silvia Corinti, Gabriella Di Felice
From Food Allergy and Anaphylaxis Meeting 2011
Venice, Italy. 17-19 February 2011
The immunological mechanisms responsible for the
anti-allergic effects of probiotic bacteria are still poorly
defined. We tested the effect of a probiotic mixture
(VSL#3) in in vitro, ex vivo and in vivo mouse systems.
In vitro co-culture of naïve bone marrow(BM)-derived
DC (BM-DC) with VSL#3 induced the up-regulation of
maturation marker and IL-10 and IL-12 production. Ex
vivo analysis of mesenteric lymph node(MLN)-derived
DC (MLN-DC) from naïve mice receiving for three
weeks VSL#3 by oral administration, indicated a differ-
ent distribution and phenotype of DC within the MLN.
In particular, VSL#3 treatment increased the frequency
of plasmacytoid DC (pDC, B220+CD11clow), and upre-
gulated the expression of maturation markers on con-
ventional DC (cDC). Moreover, the frequency of IL-10-
expressing cDC was increased. This finding was paral-
leled by the increase of CD4+CD25+ T cells and CD4
+CD25- populations showing enhanced IL-10 produc-
tion. Altogether, these results suggest that VSL#3 treat-
ment can stimulate in the gut the activation of
tolerogenic DC. Finally, we obtained in vivo preliminary
data on therapeutic and preventive potential of VSL#3
in a mouse model of sensitization and anaphylaxis to
peanut. Mice were orally sensitized and challenged with
peanut extract to induce in vivo anaphylaxis. In the
therapeutic experimental setting, animals received a
three-weeks oral treatment with VSL#3 and were then
re-challenged. In the preventive setting, oral probiotic
treatment started one week before the beginning of the
immunization schedule and continued until the
challenge. In both approaches, VSL#3 was able to
reduce anaphylaxis symptoms and IL-13 release in the
jejunum of immunized mice upon post-treatment chal-
lenge. Furthermore, the therapeutic approach also
induced allergen-specific IgA in the gut and TGF-b
release. Then, the capacity of probiotics to induce pro-
tective immune responses linked to counter-regulation
of Th2 responses might become an effective strategy in
the treatment of type I allergy.
Published: 12 August 2011
doi:10.1186/2045-7022-1-S1-O30
Cite this article as: Schiavi et al.: Probiotic treatment induces intestinal
regulatory dendritic and T cells, and counter-regulates Th2 responses
and anaphylaxis in a mouse model of food allergy. Clinical and
Translational Allergy 2011 1(Suppl 1):O30.
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Diseases, Rome, Italy
Schiavi et al. Clinical and Translational Allergy 2011, 1(Suppl 1):O30
http://www.ctajournal.com/content/1/S1/O30
© 2011 Schiavi et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


Probiotic treatment induces intestinal regulatory dendritic and T cells, and counter-regulates Th2 responses and anaphylaxis in a mouse model of food allergy

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