Babu, S Subash

Hanna, Luke Elizabeth

Jawahar, MS

Kumar, N Pavan

Nutman, Thomas B

Rekha, VV Banu

Sridhar, R

BMC Infectious Diseases

English

PubMed

N Kumar

Luke Hanna

MS Jawahar

VV Rekha

R Sridhar

Thomas B Nutman

S Babu

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Enhanced frequency of neutrophils and
inflammatory monocytes and diminished
numbers of T and B cells in active pulmonary
tuberculosis
N Pavan Kumar1,2*, Luke Elizabeth Hanna2, MS Jawahar2, VV Banu Rekha2, R Sridhar4, Thomas B Nutman3,
S Subash Babu1
From First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)
Chennai, India. 20-22 January 2012
Background
Mycobacterium tuberculosis (M.tb) infects nearly 2 bil-
lion people worldwide. Effective immunity against M.tb
infection requires co-ordinated responses from both
innate and adaptive arms of immunity. To elucidate the
immune responses important both in control of infec-
tion and in extra-pulmonary dissemination, we exam-
ined frequency and/or absolute numbers of T, B and
NK cells, dendritic cells and other leucocyte populations
in active tuberculosis patients.
Methods
The frequency as well as absolute numbers of T cells
(CD3+, CD4+, CD8+ T cells), B cells and NK cells as
well as the frequency of innate immune cells (neutro-
phils and monocytes), dendritic cell subsets (pDC &
mDC ), T cell subsets (naïve, central and effector mem-
ory and regulatory T cells) was examined by flow cyto-
metry in AFB smear positive pulmonary TB (Sm+)
(n=30) and AFB smear negative pulmonary TB (Sm-)
(n=24) and compared with extra- pulmonary TB (EP)
(n=38).
Results
Among the innate immune subsets, we observed signifi-
cantly higher frequency of neutrophils and inflammatory
monocytes in Sm+ pulmonary TB group when com-
pared with Sm- pulmonary and EP TB group. On the
other hand, the absolute numbers of CD3+ T cells, CD4
+ T cells, CD8+ T cells and B cells were significantly
lower in Sm+ when compared with Sm- and EP TB
group.
Conclusion
Pulmonary TB is characterized by enhanced frequencies
of neutrophils and inflammatory monocytes and dimin-
ished absolute counts of T and B cells, suggesting a cru-
cial role for these cell populations in protection against
TB disease development as well as extra-pulmonary
dissemination.
Author details
1National Institutes of Health – International Center for Excellence in
Research, Chennai, India. 2National Institute for Research in Tuberculosis,
Chennai, India. 3Laboratory of Parasitic Diseases, National Institutes of Allergy
and Infectious Diseases, National Institutes of Health, Bethesda, Maryland,
USA. 4Government Stanley Medical College and Hospital, Chennai, India.
Published: 4 May 2012
doi:10.1186/1471-2334-12-S1-P23
Cite this article as: Kumar et al.: Enhanced frequency of neutrophils and
inflammatory monocytes and diminished numbers of T and B cells in
active pulmonary tuberculosis. BMC Infectious Diseases 2012 12(Suppl 1):
P23.
* Correspondence: pavanmedtech@gmail.com
1National Institutes of Health – International Center for Excellence in
Research, Chennai, India
Full list of author information is available at the end of the article
Kumar et al. BMC Infectious Diseases 2012, 12(Suppl 1):P23
http://www.biomedcentral.com/1471-2334/12/S1/P23
© 2012 Kumar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


Enhanced frequency of neutrophils and inflammatory monocytes and diminished numbers of T and B cells in active pulmonary tuberculosis

N Pavan Kumar

Luke Elizabeth Hanna

VV Banu Rekha

S Subash Babu

Crossref

POSTER PRESENTATION Open AccessEnhanced frequency of neutrophils andinflammatory monocytes and diminishednumbers of T and B cells in active pulmonarytuberculosisN Pavan Kumar1,2*, Luke Elizabeth Hanna2, MS Jawahar2, VV Banu Rekha2, R Sridhar4, Thomas B Nutman3,S Subash Babu1From First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)Chennai, India. 20-22 January 2012BackgroundMycobacterium tuberculosis (M.tb) infects nearly 2 bil-lion people worldwide. Effective immunity against M.tbinfection requires co-ordinated responses from bothinnate and adaptive arms of immunity. To elucidate theimmune responses important both in control of infec-tion and in extra-pulmonary dissemination, we exam-ined frequency and/or absolute numbers of T, B andNK cells, dendritic cells and other leucocyte populationsin active tuberculosis patients.MethodsThe frequency as well as absolute numbers of T cells(CD3+, CD4+, CD8+ T cells), B cells and NK cells aswell as the frequency of innate immune cells (neutro-phils and monocytes), dendritic cell subsets (pDC &mDC ), T cell subsets (naïve, central and effector mem-ory and regulatory T cells) was examined by flow cyto-metry in AFB smear positive pulmonary TB (Sm+)(n=30) and AFB smear negative pulmonary TB (Sm-)(n=24) and compared with extra- pulmonary TB (EP)(n=38).ResultsAmong the innate immune subsets, we observed signifi-cantly higher frequency of neutrophils and inflammatorymonocytes in Sm+ pulmonary TB group when com-pared with Sm- pulmonary and EP TB group. On theother hand, the absolute numbers of CD3+ T cells, CD4+ T cells, CD8+ T cells and B cells were significantlylower in Sm+ when compared with Sm- and EP TBgroup.ConclusionPulmonary TB is characterized by enhanced frequenciesof neutrophils and inflammatory monocytes and dimin-ished absolute counts of T and B cells, suggesting a cru-cial role for these cell populations in protection againstTB disease development as well as extra-pulmonarydissemination.Author details1National Institutes of Health – International Center for Excellence inResearch, Chennai, India. 2National Institute for Research in Tuberculosis,Chennai, India. 3Laboratory of Parasitic Diseases, National Institutes of Allergyand Infectious Diseases, National Institutes of Health, Bethesda, Maryland,USA. 4Government Stanley Medical College and Hospital, Chennai, India.Published: 4 May 2012doi:10.1186/1471-2334-12-S1-P23Cite this article as: Kumar et al.: Enhanced frequency of neutrophils andinflammatory monocytes and diminished numbers of T and B cells inactive pulmonary tuberculosis. BMC Infectious Diseases 2012 12(Suppl 1):P23.* Correspondence: pavanmedtech@gmail.com1National Institutes of Health – International Center for Excellence inResearch, Chennai, IndiaFull list of author information is available at the end of the articleKumar et al. BMC Infectious Diseases 2012, 12(Suppl 1):P23http://www.biomedcentral.com/1471-2334/12/S1/P23© 2012 Kumar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.

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Enhanced frequency of neutrophils and inflammatory monocytes and diminished numbers of T and B cells in active pulmonary tuberculosis

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