Chandra, Ramesh

Kaur, Navrinder

Parkash, Braham

Ranjan, Atul

Singh, Nirpendra

Sur, Souvik

Tandon, Vibha

English

PubMed

Souvik Sur

Nirpendra Singh

Navrinder Kaur

Atul Ranjan

Braham Parkash

Ramesh Chandra

Vibha Tandon

Springer - Publisher Connector

Identification of SFPQ as novel interacting partner of HIV-1 Integrase and its functional characterization

Sur Souvik

Singh Nirpendra

Kaur Navrinder

Ranjan Atul

Parkash Braham

Chandra Ramesh

Tandon Vibha

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BMC Infectious Diseases

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POSTER PRESENTATION Open Access
Identification of SFPQ as novel interacting
partner of HIV-1 Integrase and its functional
characterization
Souvik Sur1, Nirpendra Singh2, Navrinder Kaur2, Atul Ranjan1, Braham Parkash2, Ramesh Chandra2, Vibha Tandon1*
From First International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2012)
Chennai, India. 20-22 January 2012
Background
HIV-1 requires the support of its appropriate host for the
survival and propagation like any other parasite. These
host cell factors have been reported to be involved in dif-
ferent stages of viral life cycle. The nuclear import and
infection maintenance of HIV-1 Integrase (IN) in human
cells is dependent upon the integration efficiency of the
proviral DNA and stability of viral RNA. In our study we
identified a new host cell interacting factor for HIV-1 IN,
SFPQ-a RNA splicing protein, by cross-linking, pull
down and mass spectrometry.
Methods
This study involved gene cloning, protein expression,
purification, cell culture, transfection and selection of
stable cell lines for the HIV-1 IN GFP fusion protein
and in-vitro IN activity assays study.
Results
We identified a new host cell interacting factor for HIV-
1 IN-SFPQ, an RNA splicing protein that interacts with
HIV-1, shows 5 fold binding (Kd 0.05 µM) and modu-
lates its disintegration activity.
Conclusions
This study presents SFPQ as a novel cellular factor
which has functional interaction with HIV-1 IN. SFPQ,
revealed that this protein is not only recruited to the
sites where viral genome integration takes place but also
increases the disintegration activity and 3’ end proces-
sing activities of HIV-1 IN.
Author details
1Department of Chemistry, University of Delhi, India. 2Dr. B. R. Ambedkar
Center for Biomedical Research, University of Delhi, India.
Published: 4 May 2012
doi:10.1186/1471-2334-12-S1-P80
Cite this article as: Sur et al.: Identification of SFPQ as novel interacting
partner of HIV-1 Integrase and its functional characterization. BMC
Infectious Diseases 2012 12(Suppl 1):P80.
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* Correspondence: vibhadelhi@hotmail.com
1Department of Chemistry, University of Delhi, India
Full list of author information is available at the end of the article
Sur et al. BMC Infectious Diseases 2012, 12(Suppl 1):P80
http://www.biomedcentral.com/1471-2334/12/S1/P80
© 2012 Sur et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


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Identification of SFPQ as novel interacting partner of HIV-1 Integrase and its functional characterization

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