Izdebska-Straszak, Grażyna

Krakowczyk, Łukasz

Płachetka, Anna

Strzelczyk, Joanna Katarzyna

Wiczkowski, Andrzej

English

PubMed

Joanna Strzelczyk

Łukasz Krakowczyk

Anna Płachetka

Grażyna Izdebska-Straszak

Andrzej Wiczkowski

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MEETING ABSTRACT Open Access
DNA methylation in tumor-adjacent normal tissue
in the course of head and neck squamous cell
carcinoma as an epigenetic field for cancerization
Joanna Katarzyna Strzelczyk1*, Łukasz Krakowczyk2, Anna Płachetka1, Grażyna Izdebska-Straszak1,
Andrzej Wiczkowski1
From Annual Conference on Hereditary Cancers 2011
Szczecin, Poland. 17-18 November 2011
DNA methylation is an epigenetic alteration, involved in
human cancers. “Field cancerization”, also known as
“field defect” in cancerogenesis has been explained by
the presence of cells with genetic and epigenetic altera-
tions, which are predisposed to cancer development.
DNA methylation has been proposed as a candidate
mediator of this field cancerization.
The aim of the study was to assess the methylation
status of 6 genes promoter (MGMT, APC, TIMP3,
RAR, ECAD and p16) in the tumor and in the adjacent
normal tissue in patients suffering from head and neck
squamous cell carcinoma (HNSCC).
The samples of tumor and adjacent normal tissue were
obtained during surgery from 55 patients hospitalized at
Maria Skłodowska-Curie Cancer Center and Institute of
Oncology, Gliwice. After DNA isolation the methylation-
specific polymerase chain reaction (MSP) was used to
analyze the methylation status of the gene promoter
mentioned above. Conventional histopathological para-
meters were assessed to prove the presence of squamous
cell carcinoma while no neoplastic changes were detected
histopathologically in the adjacent normal tissue. In order
to establish the clinical relevance of methylation in
HNSCC, correlation between methylation frequency of
these genes promoter as well as the clinical stage of
tumor, the patient’s age and gender were also considered.
Ethics approval for this study was received from the
Maria Skłodowska-Curie Cancer Center and Institute of
Oncology Ethics Committee (No. KB/493-31/08).
Methylation of MGMT, APC, TIMP3, RAR, ECAD
and p16 were detected in 38%, 33%, 61%, 67%, 29%,
49% of tumors, respectively. Some of the patients who
delivered methylation–positive tumor samples, had also
methylation–positive adjacent normal tissues. The genes
promoter methylation were present in 20%, 31%, 39%,
49%, 11%, 42% of the tumor-adjacent normal tissues,
respectively.
To sum up, the epigenetic modifications represented
by DNA methylation in the adjacent normal tissue in
the course of HNSCC point to the usefulness of DNA
methylation as a marker of epigenetic cancerization field
and of the future risk of cancer development.
Author details
1Chair and Department of General Biology, Medical University of Silesia,
Zabrze, Poland. 2Clinic of Oncological and Reconstructive Surgery, The Maria
Sklodowska–Curie Memorial Cancer Center and Institute of Oncology,
Gliwice, Poland.
Published: 20 April 2012
doi:10.1186/1897-4287-10-S3-A20
Cite this article as: Strzelczyk et al.: DNA methylation in tumor-adjacent
normal tissue in the course of head and neck squamous cell carcinoma
as an epigenetic field for cancerization. Hereditary Cancer in Clinical
Practice 2012 10(Suppl 3):A20.
* Correspondence: Asia.Strzelczyk@gmail.com
1Chair and Department of General Biology, Medical University of Silesia,
Zabrze, Poland
Full list of author information is available at the end of the article
Strzelczyk et al. Hereditary Cancer in Clinical Practice 2012, 10(Suppl 3):A20
http://www.hccpjournal.com/content/10/S3/A20
© 2012 Strzelczyk et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.


DNA methylation in tumor-adjacent normal tissue in the course of head and neck squamous cell carcinoma as an epigenetic field for cancerization

DNA methylation in tumor-adjacent normal tissue in the course of head and neck squamous cell carcinoma as an epigenetic field for cancerization

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