Fungal infection following placement of ventriculostomy or ventriculoperitoneal (VP) shunt is uncommon. We report the first case of Alternaria related central nervous system (CNS) shunt infection in a patient with CNS ependymoma manifesting as leptomeningitis and a spinal intradural mass. This case illustrates the diagnostic and management challenges

Chen, Mike Yue

Dadwal, Sanjeet S.

Jandial, Rahul

Tegtmeier, Bernard

Thompson, Russell

Case Reports in Infectious Diseases

English

PubMed

Sanjeet S. Dadwal

Russell Thompson

Rahul Jandial

Bernard Tegtmeier

Mike Yue Chen

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Chronic Leptomeningitis and Spinal Intradural Mass Secondary to Alternaria Infection in a Patient with Ventriculoperitoneal Shunt

Dadwal, Sanjeet S

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Washington University School of MedicineDigital Commons@BeckerOpen Access Publications2016Chronic leptomeningitis and spinal intradural masssecondary to alternaria infection in a patient withventriculoperitoneal shuntSanjeet S. DadwalCity of Hope National Medical CenterRussell ThompsonWashington University School of Medicine in St. LouisRahul JandialCity of Hope National Medical CenterBernard TegtmeierCity of Hope National Medical CenterMike Yue ChenCity of Hope National Medical CenterFollow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubsThis Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in OpenAccess Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact engeszer@wustl.edu.Recommended CitationDadwal, Sanjeet S.; Thompson, Russell; Jandial, Rahul; Tegtmeier, Bernard; and Chen, Mike Yue, ,"Chronic leptomeningitis and spinalintradural mass secondary to alternaria infection in a patient with ventriculoperitoneal shunt." Case Reports in InfectiousDiseases.2016,4693409. 1-4. (2016).https://digitalcommons.wustl.edu/open_access_pubs/6406Case ReportChronic Leptomeningitis and Spinal IntraduralMass Secondary to Alternaria Infection in a Patient withVentriculoperitoneal ShuntSanjeet S. Dadwal,1 Russell Thompson,2 Rahul Jandial,3Bernard Tegtmeier,1 andMike Yue Chen31Division of Infectious Disease, City of Hope National Medical Center, Duarte, CA, USA2Washington University School of Medicine, St. Louis, MO, USA3Division of Neurosurgery, City of Hope National Medical Center, Duarte, CA, USACorrespondence should be addressed to Sanjeet S. Dadwal; sdadwal@coh.orgReceived 12 July 2016; Accepted 3 October 2016Academic Editor: Larry M. BushCopyright © 2016 Sanjeet S. Dadwal et al. This is an open access article distributed under the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properlycited.Fungal infection following placement of ventriculostomy or ventriculoperitoneal (VP) shunt is uncommon. We report the firstcase of Alternaria related central nervous system (CNS) shunt infection in a patient with CNS ependymoma manifesting asleptomeningitis and a spinal intradural mass. This case illustrates the diagnostic and management challenges.1. IntroductionInfection is a major concern following placement of ven-triculostomy or ventriculoperitoneal (VP) shunt with ratesreported between 0 and 20%. The infection may result fromcontamination of the device during insertion or duringroutine care/manipulation and colonization at the insertionsite from skin flora [1]. Central nervous system (CNS) fungalinfections related to VP shunts are uncommon [2–4] andpose significant diagnostic and management challenges [1,4]. The CNS fungal infections may manifest acutely withdire consequences especially in those with altered immunefunction such as those in hematologic malignancies andtransplant recipients. However, in other patient populationsthe symptoms may be chronic and protean yet cause signifi-cant debilitation.2. Case ReportA48-year-oldmale presented in 9/2006 at an outside hospitalwith headaches. Magnetic resonance imaging (MRI) of thebrain revealed a posterior fossa mass. He had craniotomywith biopsy of the mass and histopathology was consistentwith grade II ependymoma. The postoperative course wascomplicated by obstructive hydrocephalus that required ven-triculostomy and one month later an occipital VP shuntwas placed. Cranial radiation therapy was administeredtotaling 54 Grays in 30 fractions to the field surroundingthe tumor bed in 10/2006. He did well for a year until hedeveloped worsening back pain and restaging MRI revealeddiffuse leptomeningeal involvement of the spine. He waspresumed to have drop metastasis and underwent wholespine radiation with a total dose of 41 Gray in 11/2007. Thebrain MRI over the following year revealed slow progressionwith extensive leptomeningeal enhancement involving theperimesencephalic cistern, bilateral foramina of Luschka,bilateral internal auditory canals, interpeduncular cistern,prepontine cisterns, quadrigeminal cistern, and right ten-torium. MRI of the spine demonstrated leptomeningealenhancement of the cervicothoracic and distal spinal cordwith a large lumbosacral intrathecal mass consistent withresidual ependymoma or postradiation scar. He was startedon temozolomide in 5/2009.Hindawi Publishing CorporationCase Reports in Infectious DiseasesVolume 2016, Article ID 4693409, 4 pageshttp://dx.doi.org/10.1155/2016/46934092 Case Reports in Infectious Diseases(a) (b)(c) (d)(e)Figure 1: ((a) & (b)) Pretreatment MRI images, (c) CSF cytology showing hyphae and inflammatory response, (d) discolored Ommayareservoir, and (e) posttreatment MRI.In June 2009, patient transferred his care to our insti-tution with the diagnosis of diffuse cranial and spinal lep-tomeningeal ependymoma with lumbosacral drop metasta-sis. He was symptomatic with numbness in his right legand back pain. He denied headaches, neck stiffness, visualchanges, fever, or chills. He was continued on temozolomide.A spinal tap in 9/2009 showed cerebral spinal fluid (CSF)pleocytosis (WBC 436, neutrophils 59%, lymphocytes 17%,monocytes 12%, and unclassified cells 12%), low glucose(39mg/dL), and high protein (636mg/dL). Cytology andcultures (bacterial, fungal, and acid fast bacilli) were negative.He completed 4 cycles of temozolomide in 12/2009 andunderwent radiation (17Gy) to the lumbosacral spine in1/2010. He was started on carboplatin and VP-16 in 1/2010 forprogression of leptomeningeal disease and completed 8 cyclesin 8/2010. AVP shunt revisionwas performed in 4/2010 alongwith multiple sessions of intracranial shunt programmingfor management of hydrocephalus. Between May and July2010 he started to complain of lower extremity weakness,urinary incontinence, and developed foot drop. In 9/2010,MRI of the brain revealed stable leptomeningeal enhance-ment surrounding the pons, midbrain, and quadrigeminalcistern. MRI of the spine showed persistent enhancementof the cervical, thoracic, and lumbar spine and a 9 cm longenhancing soft tissue mass filling the thecal sac from L3 to S1(Figures 1(a) and 1(b)).In September 2010, he had spinal decompressive surgeryfor management of L3 to S1 intradural mass that was causingCase Reports in Infectious Diseases 3symptoms of right leg weakness, urinary incontinence, andback pain. Intraoperatively, a dense mass consisting of aninseparable tangle of nerve roots and scant fibrous tissue wasidentified. Surprisingly, histopathology displayed necrotictissue with severe acute and chronic inflammationwith singleseptate hyphae. Culture of the surgical tissue was not sent.The serum Aspergillus galactomannan antigen, 1,3-beta D-glucan, Coccidioides immitis serology, Histoplasma antigen,and Blastomyces serology were negative. CSF obtained in thefirst week of October 2010 was as follows: WBC 179, RBC 3,neutrophils 78%, lymphocytes 10%, monocytes 12%, glucose73mg/dL, and protein 125mg/dL. The CSF Aspergillus galac-tomannan antigen, cryptococcal antigen, and Coccidioidesimmitis serology were negative. The bacterial, AFB, and fun-gal cultures were negative. No occult site of fungal infectionwas identified on CT scan of sinus, chest, and abdomen.Voriconazole was initiated in 10/2010 at therapeutic dosesbased on body weight. MRI of the brain and spine wasrepeated a month later that depicted improvement in theleptomeningeal enhancement with the exception of the L3-S1intradural mass, which remained unchanged. By December2010, the CSF profile improved (WBC 15, neutrophils 3%,lymphocyte 77%, monocytes 6%, protein 98mg/dL, andglucose 46mg/dL). MRI of the brain in 2/2011 displayed asignificant decrease/resolution of abnormal leptomeningealenhancement. MRI of the spine also showed near resolutionof the cervicothoracic leptomeningeal enhancement, whereasthe intradural filling defect in LS spine was unchanged. Afollow-up spinal tap in February 2011 showed worseningpleocytosis (WBC 79, neutrophils 18%, lymphocytes 49%,monocytes 17%, and eosinophils 5%), increased protein(175mg/dL), and for the first time cytology showed septatefungal hyphae with morphology suggestive of Alternaria(Figure 1(c)). The CSF culture eventually grew Alternariaspp. He was hospitalized and treated with liposomal Ampho-tericin B (L-Amb) and Voriconazole pending antifungalsusceptibility testing at a reference lab.All CNS hardware (VP shunt andOmmaya reservoir) wasremoved and an external ventricular drain (EVD)was placed.The Ommaya reservoir was discolored and a moldy blacksubstance was seen on macroscopic exam (Figure 1(d)). L-Ambwas stopped due to worsening renal function. A newVPshunt was placed for chronic obstructive hydrocephalus afterrepeated CSF in 3/2011 showed a decline in WBC to 7 cellsandwas negative for fungal growth. Based on the informationfrom the antifungal susceptibility testing (Table 1), the patientwas maintained on intravenous Voriconazole for one yearfrom March 2011 with close monitoring of Voriconazoletrough levels (the trough levels on oral formulation weresubtherapeutic despite weight based dosing).Follow-up MRI indicated no residual brain or cervi-cothoracic spinal leptomeningeal enhancement (Figure 1(e)).The L3-S1 spine intradural soft tissue mass has remainedunchanged. CSF analysis from March 2012 showed 2 WBC,2 RBC, mildly elevated protein, and normal glucose. He wasnoted to have improvement in lower extremity weakness;however, the foot drop persisted. Most recent MRI of thebrain and spine shows no interval change.Table 1: Antifungal susceptibility.Antifungal agent MIC (mg/mL)Amphotericin B 0.54Itraconazole 0.5Posaconazole 0.25Voriconazole 1.003. DiscussionAlternaria spp. are dematiaceous hyphomycetes with world-wide distribution and are commonly found in soil, wood, anddecomposing plant debris [5]. They are also found on intacthuman skin and conjunctiva [6]. Alternaria is an emergingopportunistic pathogen. It has been implicated in aller-gic rhinosinusitis, asthma, onychomycosis, ocular infectionsespecially in the setting of contact lens use/ophthalmologicsurgery, cutaneous/subcutaneous infections, especially thesolid organ transplant recipients, and, lastly, invasive diseasein patients with hematologic malignancy [6–9]. Only onecase of fatal Alternaria primary brain abscess has beenreported in a child with chronic granulomatous disease [10].Central nervous system device related fungal infectionsare an uncommon occurrence [2, 3] with themost commonlyreported isolate being Candida spp. [3, 11]. These infectionscan be difficult to diagnose and often pose managementchallenges.The difficulty in diagnosismay result from the lowyield of culture, low index of suspicion for fungal infection,and the lack of typical symptoms such as fever, headaches,neck pain, or nuchal rigidity.In this report, we have described the clinical courseof an occult fungal leptomeningeal infection caused byAlternaria spp., in a patient with a CNS malignancy thatrequired multiple surgical interventions for the managementof obstructive hydrocephalus. This created the setting forCNS device related infection, as higher infection rates arereported with secondary shunt surgery [12]. In our patientit was very difficult to determine the exact time of onset ofinfection because typical symptoms or signs of meningitiswere lacking and the findings noted onMRI were consideredasmalignancy related. CSF pleocytosis, elevated protein level,and low glucose on initial CSF suggested an inflammatoryprocess; however, microbiologic work-up was negative whichsuggested possibility of partially treated malignancy.The diagnosis of CNS fungal infection can be particularlychallenging as demonstrated by this case.Thefirst sign of fun-gal infectionwas the histologic observation of solitary hyphaewith acute and chronic inflammation in the intradural mass.Ultimately, CSF from the Ommaya reservoir grew Alternariaspp. and concurrently had an elevated CSF eosinophil count.Although Alternaria has never been reported as the cause ofchronic leptomeningitis, other dematiaceous fungi such asScedosporium, Cladophialophora, andWangiella have causeddevastating CNS infections in both immunocompetent andimmunocompromised hosts. Dimorphic fungi such asHisto-plasma and Coccidioides have been described as a cause ofchronic meningitis. Hagman et al. described case of CNSCoccidioides immitis infection manifesting as “hyphal form”4 Case Reports in Infectious Diseasesin the brain parenchyma and CSF, especially in the setting ofa VP shunt or Rickham reservoir [13].Themanagement of infectedCNS shunts is challenging. Itinvolves appropriate antimicrobial therapy (with reliable pen-etration into the CSF and brain tissue) targeting the identifiedpathogen, removal of the infected CNS shunt, and resectionof infected tissue, which is not always feasible. In our patient,the initial antifungal agent, Voriconazole, was chosen basedon the histological evidence of “septate hyphae” that sug-gested the possibility of Aspergillus, although non-Aspergilluslikemoldswere in the differential diagnosis. Voriconazole hasgood in vitro activity against Alternaria spp., and the isolatewas sensitive based on MIC breakpoint (Table 1).Long-term Voriconazole use can be complicated withproblems such as drug related hepatotoxicity and phototox-icity with risk for skin cancer. Our patient exhibited bothof these side effects with the exception of skin cancer. Wealso encountered challenge in maintaining adequate troughlevel with oral formulation that was overcome by intravenousdosing. In vitro, Amphotericin B and Posaconazole haveactivity against this pathogen and can be used as second-line agents. Amphotericin B lipid formulations can leadto nephrotoxicity, which our patient developed. There is apaucity of data and recommendations regarding the durationof antifungal therapy in such situations. We treated ourpatient for one year with intravenous Voriconazole at thera-peutic doses (weight based). Repeated CSF analyses andMRIof the brain/spine were done to measure treatment response.At the one year treatmentmark, CSF pleocytosis had resolvedand MRI indicated resolution of the brain/cervicothoracicleptomeningeal inflammation. However, the lumbar intradu-ral spine mass remained unchanged. Clinically, the patienthas regained functional capacity, although lower extremityneurological problems persist.4. ConclusionThis case description emphasizes the challenges faced inestablishing the diagnosis of a CNS device related fungalinfection and its management in the setting of a CNSmalignancy.Competing InterestsThe authors declare that there is no conflict of interestsregarding the publication of this paper.References[1] E. Stenehjem and W. S. Armstrong, “Central nervous systemdevice infections,” Infectious Disease Clinics of North America,vol. 26, no. 1, pp. 89–110, 2012.[2] J. Berenguer, J. Diaz-Mediavilla, D. Urra, and P. Mun˜oz, “Cen-tral nervous system infection caused by Pseudallescheria boydii:case report and review,” Reviews of Infectious Diseases, vol. 11,no. 6, pp. 890–896, 1989.[3] D. O’Brien, N. T. Stevens, C. H. Lim et al., “Candida infectionof the central nervous system following neurosurgery: a 12-yearreview,”ActaNeurochirurgica, vol. 153, no. 6, pp. 1347–1350, 2011.[4] B. Peters, T. Faggett, and C. C. Sampson, “Isolation ofMonospo-rium apiospermum from cerebrospinal fluid,” Journal of theNational Medical Association, vol. 68, no. 6, pp. 512–513, 1976.[5] M. E. Brandt and D.W.Warnock, “Epidemiology, clinical man-ifestations, and therapy of infections caused by dematiaceousfungi,” Journal of Chemotherapy, vol. 15, no. 2, pp. 36–47, 2003.[6] F. J. Pastor and J. Guarro, “Alternaria infections: laboratorydiagnosis and relevant clinical features,” Clinical Microbiologyand Infection, vol. 14, no. 8, pp. 734–746, 2008.[7] P. C. Iwen, M. E. Rupp, and S. H. Hinrichs, “Invasive moldsinusitis: 17 cases in immunocompromised patients and reviewof the literature,” Clinical Infectious Diseases, vol. 24, no. 6, pp.1178–1184, 1997.[8] V. A. Morrison and D. J. Weisdorf, “Alternaria: a sinonasalpathogen of immunocompromised hosts,” Clinical InfectiousDiseases, vol. 16, no. 2, pp. 265–270, 1993.[9] S. E. M. Vermeire, H. de Jonge, K. Lagrou, and D. R. J. Kuypers,“Cutaneous phaeohyphomycosis in renal allograft recipients:report of 2 cases and review of the literature,” DiagnosticMicrobiology and Infectious Disease, vol. 68, no. 2, pp. 177–180,2010.[10] E. Hipolito, E. Faria, A. F. Alves et al., “Alternaria infectoriabrain abscess in a child with chronic granulomatous disease,”European Journal of Clinical Microbiology and Infectious Dis-eases, vol. 28, no. 4, pp. 377–380, 2009.[11] A. Angel-Moreno, A. France´s, J. M. Granado, and J. L. Pe´rez-Arellano, “Ventriculoperitoneal shunt infection by Candidaglabrata in an adult,” Journal of Infection, vol. 41, no. 2, pp. 178–179, 2000.[12] A.-M. Korinek, L. Fulla-Oller, A.-L. Boch, J.-L. Golmard,B. Hadiji, and L. Puybasset, “Morbidity of ventricular cere-brospinal fluid shunt surgery in adults: an 8-year study,” Neu-rosurgery, vol. 68, no. 4, pp. 985–994, 2011.[13] H. M. Hagman, E. G. Madnick, A. N. D’Agostino et al.,“Hyphal forms in the central nervous system of patients withcoccidioidomycosis,” Clinical Infectious Diseases, vol. 30, no. 2,pp. 349–355, 2000.Submit your manuscripts athttp://www.hindawi.comStem CellsInternationalHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014MEDIATORSINFLAMMATIONofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Behavioural NeurologyEndocrinologyInternational Journal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Disease MarkersHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014BioMed Research InternationalOncologyJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Oxidative Medicine and Cellular LongevityHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014PPAR ResearchThe Scientific World JournalHindawi Publishing Corporation http://www.hindawi.com Volume 2014Immunology ResearchHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Journal ofObesityJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014 Computational and  Mathematical Methods in MedicineOphthalmologyJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Diabetes ResearchJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Research and TreatmentAIDSHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Gastroenterology Research and PracticeHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Parkinson’s DiseaseEvidence-Based Complementary and Alternative MedicineVolume 2014Hindawi Publishing Corporationhttp://www.hindawi.com

Chronic leptomeningitis and spinal intradural mass secondary to alternaria infection in a patient with ventriculoperitoneal shunt

Fungal infection following placement of ventriculostomy or ventriculoperitoneal (VP) shunt is uncommon. We report the first case of Alternaria related central nervous system (CNS) shunt infection in a patient with CNS ependymoma manifesting as leptomeningitis and a spinal intradural mass. This case illustrates the diagnostic and management challenges.</jats:p

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Case ReportChronic Leptomeningitis and Spinal IntraduralMass Secondary to Alternaria Infection in a Patient withVentriculoperitoneal ShuntSanjeet S. Dadwal,1 Russell Thompson,2 Rahul Jandial,3Bernard Tegtmeier,1 andMike Yue Chen31Division of Infectious Disease, City of Hope National Medical Center, Duarte, CA, USA2Washington University School of Medicine, St. Louis, MO, USA3Division of Neurosurgery, City of Hope National Medical Center, Duarte, CA, USACorrespondence should be addressed to Sanjeet S. Dadwal; sdadwal@coh.orgReceived 12 July 2016; Accepted 3 October 2016Academic Editor: Larry M. BushCopyright © 2016 Sanjeet S. Dadwal et al. This is an open access article distributed under the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properlycited.Fungal infection following placement of ventriculostomy or ventriculoperitoneal (VP) shunt is uncommon. We report the firstcase of Alternaria related central nervous system (CNS) shunt infection in a patient with CNS ependymoma manifesting asleptomeningitis and a spinal intradural mass. This case illustrates the diagnostic and management challenges.1. IntroductionInfection is a major concern following placement of ven-triculostomy or ventriculoperitoneal (VP) shunt with ratesreported between 0 and 20%. The infection may result fromcontamination of the device during insertion or duringroutine care/manipulation and colonization at the insertionsite from skin flora [1]. Central nervous system (CNS) fungalinfections related to VP shunts are uncommon [2–4] andpose significant diagnostic and management challenges [1,4]. The CNS fungal infections may manifest acutely withdire consequences especially in those with altered immunefunction such as those in hematologic malignancies andtransplant recipients. However, in other patient populationsthe symptoms may be chronic and protean yet cause signifi-cant debilitation.2. Case ReportA48-year-oldmale presented in 9/2006 at an outside hospitalwith headaches. Magnetic resonance imaging (MRI) of thebrain revealed a posterior fossa mass. He had craniotomywith biopsy of the mass and histopathology was consistentwith grade II ependymoma. The postoperative course wascomplicated by obstructive hydrocephalus that required ven-triculostomy and one month later an occipital VP shuntwas placed. Cranial radiation therapy was administeredtotaling 54 Grays in 30 fractions to the field surroundingthe tumor bed in 10/2006. He did well for a year until hedeveloped worsening back pain and restaging MRI revealeddiffuse leptomeningeal involvement of the spine. He waspresumed to have drop metastasis and underwent wholespine radiation with a total dose of 41 Gray in 11/2007. Thebrain MRI over the following year revealed slow progressionwith extensive leptomeningeal enhancement involving theperimesencephalic cistern, bilateral foramina of Luschka,bilateral internal auditory canals, interpeduncular cistern,prepontine cisterns, quadrigeminal cistern, and right ten-torium. MRI of the spine demonstrated leptomeningealenhancement of the cervicothoracic and distal spinal cordwith a large lumbosacral intrathecal mass consistent withresidual ependymoma or postradiation scar. He was startedon temozolomide in 5/2009.Hindawi Publishing CorporationCase Reports in Infectious DiseasesVolume 2016, Article ID 4693409, 4 pageshttp://dx.doi.org/10.1155/2016/46934092 Case Reports in Infectious Diseases(a) (b)(c) (d)(e)Figure 1: ((a) & (b)) Pretreatment MRI images, (c) CSF cytology showing hyphae and inflammatory response, (d) discolored Ommayareservoir, and (e) posttreatment MRI.In June 2009, patient transferred his care to our insti-tution with the diagnosis of diffuse cranial and spinal lep-tomeningeal ependymoma with lumbosacral drop metasta-sis. He was symptomatic with numbness in his right legand back pain. He denied headaches, neck stiffness, visualchanges, fever, or chills. He was continued on temozolomide.A spinal tap in 9/2009 showed cerebral spinal fluid (CSF)pleocytosis (WBC 436, neutrophils 59%, lymphocytes 17%,monocytes 12%, and unclassified cells 12%), low glucose(39mg/dL), and high protein (636mg/dL). Cytology andcultures (bacterial, fungal, and acid fast bacilli) were negative.He completed 4 cycles of temozolomide in 12/2009 andunderwent radiation (17Gy) to the lumbosacral spine in1/2010. He was started on carboplatin and VP-16 in 1/2010 forprogression of leptomeningeal disease and completed 8 cyclesin 8/2010. AVP shunt revisionwas performed in 4/2010 alongwith multiple sessions of intracranial shunt programmingfor management of hydrocephalus. Between May and July2010 he started to complain of lower extremity weakness,urinary incontinence, and developed foot drop. In 9/2010,MRI of the brain revealed stable leptomeningeal enhance-ment surrounding the pons, midbrain, and quadrigeminalcistern. MRI of the spine showed persistent enhancementof the cervical, thoracic, and lumbar spine and a 9 cm longenhancing soft tissue mass filling the thecal sac from L3 to S1(Figures 1(a) and 1(b)).In September 2010, he had spinal decompressive surgeryfor management of L3 to S1 intradural mass that was causingCase Reports in Infectious Diseases 3symptoms of right leg weakness, urinary incontinence, andback pain. Intraoperatively, a dense mass consisting of aninseparable tangle of nerve roots and scant fibrous tissue wasidentified. Surprisingly, histopathology displayed necrotictissue with severe acute and chronic inflammationwith singleseptate hyphae. Culture of the surgical tissue was not sent.The serum Aspergillus galactomannan antigen, 1,3-beta D-glucan, Coccidioides immitis serology, Histoplasma antigen,and Blastomyces serology were negative. CSF obtained in thefirst week of October 2010 was as follows: WBC 179, RBC 3,neutrophils 78%, lymphocytes 10%, monocytes 12%, glucose73mg/dL, and protein 125mg/dL. The CSF Aspergillus galac-tomannan antigen, cryptococcal antigen, and Coccidioidesimmitis serology were negative. The bacterial, AFB, and fun-gal cultures were negative. No occult site of fungal infectionwas identified on CT scan of sinus, chest, and abdomen.Voriconazole was initiated in 10/2010 at therapeutic dosesbased on body weight. MRI of the brain and spine wasrepeated a month later that depicted improvement in theleptomeningeal enhancement with the exception of the L3-S1intradural mass, which remained unchanged. By December2010, the CSF profile improved (WBC 15, neutrophils 3%,lymphocyte 77%, monocytes 6%, protein 98mg/dL, andglucose 46mg/dL). MRI of the brain in 2/2011 displayed asignificant decrease/resolution of abnormal leptomeningealenhancement. MRI of the spine also showed near resolutionof the cervicothoracic leptomeningeal enhancement, whereasthe intradural filling defect in LS spine was unchanged. Afollow-up spinal tap in February 2011 showed worseningpleocytosis (WBC 79, neutrophils 18%, lymphocytes 49%,monocytes 17%, and eosinophils 5%), increased protein(175mg/dL), and for the first time cytology showed septatefungal hyphae with morphology suggestive of Alternaria(Figure 1(c)). The CSF culture eventually grew Alternariaspp. He was hospitalized and treated with liposomal Ampho-tericin B (L-Amb) and Voriconazole pending antifungalsusceptibility testing at a reference lab.All CNS hardware (VP shunt andOmmaya reservoir) wasremoved and an external ventricular drain (EVD)was placed.The Ommaya reservoir was discolored and a moldy blacksubstance was seen on macroscopic exam (Figure 1(d)). L-Ambwas stopped due to worsening renal function. A newVPshunt was placed for chronic obstructive hydrocephalus afterrepeated CSF in 3/2011 showed a decline in WBC to 7 cellsandwas negative for fungal growth. Based on the informationfrom the antifungal susceptibility testing (Table 1), the patientwas maintained on intravenous Voriconazole for one yearfrom March 2011 with close monitoring of Voriconazoletrough levels (the trough levels on oral formulation weresubtherapeutic despite weight based dosing).Follow-up MRI indicated no residual brain or cervi-cothoracic spinal leptomeningeal enhancement (Figure 1(e)).The L3-S1 spine intradural soft tissue mass has remainedunchanged. CSF analysis from March 2012 showed 2 WBC,2 RBC, mildly elevated protein, and normal glucose. He wasnoted to have improvement in lower extremity weakness;however, the foot drop persisted. Most recent MRI of thebrain and spine shows no interval change.Table 1: Antifungal susceptibility.Antifungal agent MIC (mg/mL)Amphotericin B 0.54Itraconazole 0.5Posaconazole 0.25Voriconazole 1.003. DiscussionAlternaria spp. are dematiaceous hyphomycetes with world-wide distribution and are commonly found in soil, wood, anddecomposing plant debris [5]. They are also found on intacthuman skin and conjunctiva [6]. Alternaria is an emergingopportunistic pathogen. It has been implicated in aller-gic rhinosinusitis, asthma, onychomycosis, ocular infectionsespecially in the setting of contact lens use/ophthalmologicsurgery, cutaneous/subcutaneous infections, especially thesolid organ transplant recipients, and, lastly, invasive diseasein patients with hematologic malignancy [6–9]. Only onecase of fatal Alternaria primary brain abscess has beenreported in a child with chronic granulomatous disease [10].Central nervous system device related fungal infectionsare an uncommon occurrence [2, 3] with themost commonlyreported isolate being Candida spp. [3, 11]. These infectionscan be difficult to diagnose and often pose managementchallenges.The difficulty in diagnosismay result from the lowyield of culture, low index of suspicion for fungal infection,and the lack of typical symptoms such as fever, headaches,neck pain, or nuchal rigidity.In this report, we have described the clinical courseof an occult fungal leptomeningeal infection caused byAlternaria spp., in a patient with a CNS malignancy thatrequired multiple surgical interventions for the managementof obstructive hydrocephalus. This created the setting forCNS device related infection, as higher infection rates arereported with secondary shunt surgery [12]. In our patientit was very difficult to determine the exact time of onset ofinfection because typical symptoms or signs of meningitiswere lacking and the findings noted onMRI were consideredasmalignancy related. CSF pleocytosis, elevated protein level,and low glucose on initial CSF suggested an inflammatoryprocess; however, microbiologic work-up was negative whichsuggested possibility of partially treated malignancy.The diagnosis of CNS fungal infection can be particularlychallenging as demonstrated by this case.Thefirst sign of fun-gal infectionwas the histologic observation of solitary hyphaewith acute and chronic inflammation in the intradural mass.Ultimately, CSF from the Ommaya reservoir grew Alternariaspp. and concurrently had an elevated CSF eosinophil count.Although Alternaria has never been reported as the cause ofchronic leptomeningitis, other dematiaceous fungi such asScedosporium, Cladophialophora, andWangiella have causeddevastating CNS infections in both immunocompetent andimmunocompromised hosts. Dimorphic fungi such asHisto-plasma and Coccidioides have been described as a cause ofchronic meningitis. Hagman et al. described case of CNSCoccidioides immitis infection manifesting as “hyphal form”4 Case Reports in Infectious Diseasesin the brain parenchyma and CSF, especially in the setting ofa VP shunt or Rickham reservoir [13].Themanagement of infectedCNS shunts is challenging. Itinvolves appropriate antimicrobial therapy (with reliable pen-etration into the CSF and brain tissue) targeting the identifiedpathogen, removal of the infected CNS shunt, and resectionof infected tissue, which is not always feasible. In our patient,the initial antifungal agent, Voriconazole, was chosen basedon the histological evidence of “septate hyphae” that sug-gested the possibility of Aspergillus, although non-Aspergilluslikemoldswere in the differential diagnosis. Voriconazole hasgood in vitro activity against Alternaria spp., and the isolatewas sensitive based on MIC breakpoint (Table 1).Long-term Voriconazole use can be complicated withproblems such as drug related hepatotoxicity and phototox-icity with risk for skin cancer. Our patient exhibited bothof these side effects with the exception of skin cancer. Wealso encountered challenge in maintaining adequate troughlevel with oral formulation that was overcome by intravenousdosing. In vitro, Amphotericin B and Posaconazole haveactivity against this pathogen and can be used as second-line agents. Amphotericin B lipid formulations can leadto nephrotoxicity, which our patient developed. There is apaucity of data and recommendations regarding the durationof antifungal therapy in such situations. We treated ourpatient for one year with intravenous Voriconazole at thera-peutic doses (weight based). Repeated CSF analyses andMRIof the brain/spine were done to measure treatment response.At the one year treatmentmark, CSF pleocytosis had resolvedand MRI indicated resolution of the brain/cervicothoracicleptomeningeal inflammation. However, the lumbar intradu-ral spine mass remained unchanged. Clinically, the patienthas regained functional capacity, although lower extremityneurological problems persist.4. ConclusionThis case description emphasizes the challenges faced inestablishing the diagnosis of a CNS device related fungalinfection and its management in the setting of a CNSmalignancy.Competing InterestsThe authors declare that there is no conflict of interestsregarding the publication of this paper.References[1] E. Stenehjem and W. S. Armstrong, “Central nervous systemdevice infections,” Infectious Disease Clinics of North America,vol. 26, no. 1, pp. 89–110, 2012.[2] J. Berenguer, J. Diaz-Mediavilla, D. Urra, and P. Mun˜oz, “Cen-tral nervous system infection caused by Pseudallescheria boydii:case report and review,” Reviews of Infectious Diseases, vol. 11,no. 6, pp. 890–896, 1989.[3] D. O’Brien, N. T. Stevens, C. H. Lim et al., “Candida infectionof the central nervous system following neurosurgery: a 12-yearreview,”ActaNeurochirurgica, vol. 153, no. 6, pp. 1347–1350, 2011.[4] B. Peters, T. Faggett, and C. C. Sampson, “Isolation ofMonospo-rium apiospermum from cerebrospinal fluid,” Journal of theNational Medical Association, vol. 68, no. 6, pp. 512–513, 1976.[5] M. E. Brandt and D.W.Warnock, “Epidemiology, clinical man-ifestations, and therapy of infections caused by dematiaceousfungi,” Journal of Chemotherapy, vol. 15, no. 2, pp. 36–47, 2003.[6] F. J. Pastor and J. Guarro, “Alternaria infections: laboratorydiagnosis and relevant clinical features,” Clinical Microbiologyand Infection, vol. 14, no. 8, pp. 734–746, 2008.[7] P. C. Iwen, M. E. Rupp, and S. H. Hinrichs, “Invasive moldsinusitis: 17 cases in immunocompromised patients and reviewof the literature,” Clinical Infectious Diseases, vol. 24, no. 6, pp.1178–1184, 1997.[8] V. A. Morrison and D. J. Weisdorf, “Alternaria: a sinonasalpathogen of immunocompromised hosts,” Clinical InfectiousDiseases, vol. 16, no. 2, pp. 265–270, 1993.[9] S. E. M. Vermeire, H. de Jonge, K. Lagrou, and D. R. J. Kuypers,“Cutaneous phaeohyphomycosis in renal allograft recipients:report of 2 cases and review of the literature,” DiagnosticMicrobiology and Infectious Disease, vol. 68, no. 2, pp. 177–180,2010.[10] E. Hipolito, E. Faria, A. F. Alves et al., “Alternaria infectoriabrain abscess in a child with chronic granulomatous disease,”European Journal of Clinical Microbiology and Infectious Dis-eases, vol. 28, no. 4, pp. 377–380, 2009.[11] A. Angel-Moreno, A. France´s, J. M. Granado, and J. L. Pe´rez-Arellano, “Ventriculoperitoneal shunt infection by Candidaglabrata in an adult,” Journal of Infection, vol. 41, no. 2, pp. 178–179, 2000.[12] A.-M. Korinek, L. Fulla-Oller, A.-L. Boch, J.-L. Golmard,B. Hadiji, and L. Puybasset, “Morbidity of ventricular cere-brospinal fluid shunt surgery in adults: an 8-year study,” Neu-rosurgery, vol. 68, no. 4, pp. 985–994, 2011.[13] H. M. Hagman, E. G. Madnick, A. N. D’Agostino et al.,“Hyphal forms in the central nervous system of patients withcoccidioidomycosis,” Clinical Infectious Diseases, vol. 30, no. 2,pp. 349–355, 2000.Submit your manuscripts athttp://www.hindawi.comStem CellsInternationalHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014MEDIATORSINFLAMMATIONofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Behavioural NeurologyEndocrinologyInternational Journal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Disease MarkersHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014BioMed Research InternationalOncologyJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Oxidative Medicine and Cellular LongevityHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014PPAR ResearchThe Scientific World JournalHindawi Publishing Corporation http://www.hindawi.com Volume 2014Immunology ResearchHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Journal ofObesityJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014 Computational and  Mathematical Methods in MedicineOphthalmologyJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Diabetes ResearchJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Research and TreatmentAIDSHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Gastroenterology Research and PracticeHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Parkinson’s DiseaseEvidence-Based Complementary and Alternative MedicineVolume 2014Hindawi Publishing Corporationhttp://www.hindawi.com

Chronic Leptomeningitis and Spinal Intradural Mass Secondary to<i> Alternaria</i> Infection in a Patient with Ventriculoperitoneal Shunt

Washington University School of MedicineDigital Commons@BeckerOpen Access Publications2016Chronic leptomeningitis and spinal intradural masssecondary to Alternaria infection in a patient withventriculoperitoneal shuntSanjeet S. DadwalRussell ThompsonRahul JandialBernard TegtmeierMike Yue ChenFollow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubsCase ReportChronic Leptomeningitis and Spinal IntraduralMass Secondary to Alternaria Infection in a Patient withVentriculoperitoneal ShuntSanjeet S. Dadwal,1 Russell Thompson,2 Rahul Jandial,3Bernard Tegtmeier,1 andMike Yue Chen31Division of Infectious Disease, City of Hope National Medical Center, Duarte, CA, USA2Washington University School of Medicine, St. Louis, MO, USA3Division of Neurosurgery, City of Hope National Medical Center, Duarte, CA, USACorrespondence should be addressed to Sanjeet S. Dadwal; sdadwal@coh.orgReceived 12 July 2016; Accepted 3 October 2016Academic Editor: Larry M. BushCopyright © 2016 Sanjeet S. Dadwal et al. This is an open access article distributed under the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properlycited.Fungal infection following placement of ventriculostomy or ventriculoperitoneal (VP) shunt is uncommon. We report the firstcase of Alternaria related central nervous system (CNS) shunt infection in a patient with CNS ependymoma manifesting asleptomeningitis and a spinal intradural mass. This case illustrates the diagnostic and management challenges.1. IntroductionInfection is a major concern following placement of ven-triculostomy or ventriculoperitoneal (VP) shunt with ratesreported between 0 and 20%. The infection may result fromcontamination of the device during insertion or duringroutine care/manipulation and colonization at the insertionsite from skin flora [1]. Central nervous system (CNS) fungalinfections related to VP shunts are uncommon [2–4] andpose significant diagnostic and management challenges [1,4]. The CNS fungal infections may manifest acutely withdire consequences especially in those with altered immunefunction such as those in hematologic malignancies andtransplant recipients. However, in other patient populationsthe symptoms may be chronic and protean yet cause signifi-cant debilitation.2. Case ReportA48-year-oldmale presented in 9/2006 at an outside hospitalwith headaches. Magnetic resonance imaging (MRI) of thebrain revealed a posterior fossa mass. He had craniotomywith biopsy of the mass and histopathology was consistentwith grade II ependymoma. The postoperative course wascomplicated by obstructive hydrocephalus that required ven-triculostomy and one month later an occipital VP shuntwas placed. Cranial radiation therapy was administeredtotaling 54 Grays in 30 fractions to the field surroundingthe tumor bed in 10/2006. He did well for a year until hedeveloped worsening back pain and restaging MRI revealeddiffuse leptomeningeal involvement of the spine. He waspresumed to have drop metastasis and underwent wholespine radiation with a total dose of 41 Gray in 11/2007. Thebrain MRI over the following year revealed slow progressionwith extensive leptomeningeal enhancement involving theperimesencephalic cistern, bilateral foramina of Luschka,bilateral internal auditory canals, interpeduncular cistern,prepontine cisterns, quadrigeminal cistern, and right ten-torium. MRI of the spine demonstrated leptomeningealenhancement of the cervicothoracic and distal spinal cordwith a large lumbosacral intrathecal mass consistent withresidual ependymoma or postradiation scar. He was startedon temozolomide in 5/2009.Hindawi Publishing CorporationCase Reports in Infectious DiseasesVolume 2016, Article ID 4693409, 4 pageshttp://dx.doi.org/10.1155/2016/46934092 Case Reports in Infectious Diseases(a) (b)(c) (d)(e)Figure 1: ((a) & (b)) Pretreatment MRI images, (c) CSF cytology showing hyphae and inflammatory response, (d) discolored Ommayareservoir, and (e) posttreatment MRI.In June 2009, patient transferred his care to our insti-tution with the diagnosis of diffuse cranial and spinal lep-tomeningeal ependymoma with lumbosacral drop metasta-sis. He was symptomatic with numbness in his right legand back pain. He denied headaches, neck stiffness, visualchanges, fever, or chills. He was continued on temozolomide.A spinal tap in 9/2009 showed cerebral spinal fluid (CSF)pleocytosis (WBC 436, neutrophils 59%, lymphocytes 17%,monocytes 12%, and unclassified cells 12%), low glucose(39mg/dL), and high protein (636mg/dL). Cytology andcultures (bacterial, fungal, and acid fast bacilli) were negative.He completed 4 cycles of temozolomide in 12/2009 andunderwent radiation (17Gy) to the lumbosacral spine in1/2010. He was started on carboplatin and VP-16 in 1/2010 forprogression of leptomeningeal disease and completed 8 cyclesin 8/2010. AVP shunt revisionwas performed in 4/2010 alongwith multiple sessions of intracranial shunt programmingfor management of hydrocephalus. Between May and July2010 he started to complain of lower extremity weakness,urinary incontinence, and developed foot drop. In 9/2010,MRI of the brain revealed stable leptomeningeal enhance-ment surrounding the pons, midbrain, and quadrigeminalcistern. MRI of the spine showed persistent enhancementof the cervical, thoracic, and lumbar spine and a 9 cm longenhancing soft tissue mass filling the thecal sac from L3 to S1(Figures 1(a) and 1(b)).In September 2010, he had spinal decompressive surgeryfor management of L3 to S1 intradural mass that was causingCase Reports in Infectious Diseases 3symptoms of right leg weakness, urinary incontinence, andback pain. Intraoperatively, a dense mass consisting of aninseparable tangle of nerve roots and scant fibrous tissue wasidentified. Surprisingly, histopathology displayed necrotictissue with severe acute and chronic inflammationwith singleseptate hyphae. Culture of the surgical tissue was not sent.The serum Aspergillus galactomannan antigen, 1,3-beta D-glucan, Coccidioides immitis serology, Histoplasma antigen,and Blastomyces serology were negative. CSF obtained in thefirst week of October 2010 was as follows: WBC 179, RBC 3,neutrophils 78%, lymphocytes 10%, monocytes 12%, glucose73mg/dL, and protein 125mg/dL. The CSF Aspergillus galac-tomannan antigen, cryptococcal antigen, and Coccidioidesimmitis serology were negative. The bacterial, AFB, and fun-gal cultures were negative. No occult site of fungal infectionwas identified on CT scan of sinus, chest, and abdomen.Voriconazole was initiated in 10/2010 at therapeutic dosesbased on body weight. MRI of the brain and spine wasrepeated a month later that depicted improvement in theleptomeningeal enhancement with the exception of the L3-S1intradural mass, which remained unchanged. By December2010, the CSF profile improved (WBC 15, neutrophils 3%,lymphocyte 77%, monocytes 6%, protein 98mg/dL, andglucose 46mg/dL). MRI of the brain in 2/2011 displayed asignificant decrease/resolution of abnormal leptomeningealenhancement. MRI of the spine also showed near resolutionof the cervicothoracic leptomeningeal enhancement, whereasthe intradural filling defect in LS spine was unchanged. Afollow-up spinal tap in February 2011 showed worseningpleocytosis (WBC 79, neutrophils 18%, lymphocytes 49%,monocytes 17%, and eosinophils 5%), increased protein(175mg/dL), and for the first time cytology showed septatefungal hyphae with morphology suggestive of Alternaria(Figure 1(c)). The CSF culture eventually grew Alternariaspp. He was hospitalized and treated with liposomal Ampho-tericin B (L-Amb) and Voriconazole pending antifungalsusceptibility testing at a reference lab.All CNS hardware (VP shunt andOmmaya reservoir) wasremoved and an external ventricular drain (EVD)was placed.The Ommaya reservoir was discolored and a moldy blacksubstance was seen on macroscopic exam (Figure 1(d)). L-Ambwas stopped due to worsening renal function. A newVPshunt was placed for chronic obstructive hydrocephalus afterrepeated CSF in 3/2011 showed a decline in WBC to 7 cellsandwas negative for fungal growth. Based on the informationfrom the antifungal susceptibility testing (Table 1), the patientwas maintained on intravenous Voriconazole for one yearfrom March 2011 with close monitoring of Voriconazoletrough levels (the trough levels on oral formulation weresubtherapeutic despite weight based dosing).Follow-up MRI indicated no residual brain or cervi-cothoracic spinal leptomeningeal enhancement (Figure 1(e)).The L3-S1 spine intradural soft tissue mass has remainedunchanged. CSF analysis from March 2012 showed 2 WBC,2 RBC, mildly elevated protein, and normal glucose. He wasnoted to have improvement in lower extremity weakness;however, the foot drop persisted. Most recent MRI of thebrain and spine shows no interval change.Table 1: Antifungal susceptibility.Antifungal agent MIC (mg/mL)Amphotericin B 0.54Itraconazole 0.5Posaconazole 0.25Voriconazole 1.003. DiscussionAlternaria spp. are dematiaceous hyphomycetes with world-wide distribution and are commonly found in soil, wood, anddecomposing plant debris [5]. They are also found on intacthuman skin and conjunctiva [6]. Alternaria is an emergingopportunistic pathogen. It has been implicated in aller-gic rhinosinusitis, asthma, onychomycosis, ocular infectionsespecially in the setting of contact lens use/ophthalmologicsurgery, cutaneous/subcutaneous infections, especially thesolid organ transplant recipients, and, lastly, invasive diseasein patients with hematologic malignancy [6–9]. Only onecase of fatal Alternaria primary brain abscess has beenreported in a child with chronic granulomatous disease [10].Central nervous system device related fungal infectionsare an uncommon occurrence [2, 3] with themost commonlyreported isolate being Candida spp. [3, 11]. These infectionscan be difficult to diagnose and often pose managementchallenges.The difficulty in diagnosismay result from the lowyield of culture, low index of suspicion for fungal infection,and the lack of typical symptoms such as fever, headaches,neck pain, or nuchal rigidity.In this report, we have described the clinical courseof an occult fungal leptomeningeal infection caused byAlternaria spp., in a patient with a CNS malignancy thatrequired multiple surgical interventions for the managementof obstructive hydrocephalus. This created the setting forCNS device related infection, as higher infection rates arereported with secondary shunt surgery [12]. In our patientit was very difficult to determine the exact time of onset ofinfection because typical symptoms or signs of meningitiswere lacking and the findings noted onMRI were consideredasmalignancy related. CSF pleocytosis, elevated protein level,and low glucose on initial CSF suggested an inflammatoryprocess; however, microbiologic work-up was negative whichsuggested possibility of partially treated malignancy.The diagnosis of CNS fungal infection can be particularlychallenging as demonstrated by this case.Thefirst sign of fun-gal infectionwas the histologic observation of solitary hyphaewith acute and chronic inflammation in the intradural mass.Ultimately, CSF from the Ommaya reservoir grew Alternariaspp. and concurrently had an elevated CSF eosinophil count.Although Alternaria has never been reported as the cause ofchronic leptomeningitis, other dematiaceous fungi such asScedosporium, Cladophialophora, andWangiella have causeddevastating CNS infections in both immunocompetent andimmunocompromised hosts. Dimorphic fungi such asHisto-plasma and Coccidioides have been described as a cause ofchronic meningitis. Hagman et al. described case of CNSCoccidioides immitis infection manifesting as “hyphal form”4 Case Reports in Infectious Diseasesin the brain parenchyma and CSF, especially in the setting ofa VP shunt or Rickham reservoir [13].Themanagement of infectedCNS shunts is challenging. Itinvolves appropriate antimicrobial therapy (with reliable pen-etration into the CSF and brain tissue) targeting the identifiedpathogen, removal of the infected CNS shunt, and resectionof infected tissue, which is not always feasible. In our patient,the initial antifungal agent, Voriconazole, was chosen basedon the histological evidence of “septate hyphae” that sug-gested the possibility of Aspergillus, although non-Aspergilluslikemoldswere in the differential diagnosis. Voriconazole hasgood in vitro activity against Alternaria spp., and the isolatewas sensitive based on MIC breakpoint (Table 1).Long-term Voriconazole use can be complicated withproblems such as drug related hepatotoxicity and phototox-icity with risk for skin cancer. Our patient exhibited bothof these side effects with the exception of skin cancer. Wealso encountered challenge in maintaining adequate troughlevel with oral formulation that was overcome by intravenousdosing. In vitro, Amphotericin B and Posaconazole haveactivity against this pathogen and can be used as second-line agents. Amphotericin B lipid formulations can leadto nephrotoxicity, which our patient developed. There is apaucity of data and recommendations regarding the durationof antifungal therapy in such situations. We treated ourpatient for one year with intravenous Voriconazole at thera-peutic doses (weight based). Repeated CSF analyses andMRIof the brain/spine were done to measure treatment response.At the one year treatmentmark, CSF pleocytosis had resolvedand MRI indicated resolution of the brain/cervicothoracicleptomeningeal inflammation. However, the lumbar intradu-ral spine mass remained unchanged. Clinically, the patienthas regained functional capacity, although lower extremityneurological problems persist.4. ConclusionThis case description emphasizes the challenges faced inestablishing the diagnosis of a CNS device related fungalinfection and its management in the setting of a CNSmalignancy.Competing InterestsThe authors declare that there is no conflict of interestsregarding the publication of this paper.References[1] E. Stenehjem and W. S. Armstrong, “Central nervous systemdevice infections,” Infectious Disease Clinics of North America,vol. 26, no. 1, pp. 89–110, 2012.[2] J. Berenguer, J. Diaz-Mediavilla, D. Urra, and P. Mun˜oz, “Cen-tral nervous system infection caused by Pseudallescheria boydii:case report and review,” Reviews of Infectious Diseases, vol. 11,no. 6, pp. 890–896, 1989.[3] D. O’Brien, N. T. Stevens, C. H. Lim et al., “Candida infectionof the central nervous system following neurosurgery: a 12-yearreview,”ActaNeurochirurgica, vol. 153, no. 6, pp. 1347–1350, 2011.[4] B. Peters, T. Faggett, and C. C. Sampson, “Isolation ofMonospo-rium apiospermum from cerebrospinal fluid,” Journal of theNational Medical Association, vol. 68, no. 6, pp. 512–513, 1976.[5] M. E. Brandt and D.W.Warnock, “Epidemiology, clinical man-ifestations, and therapy of infections caused by dematiaceousfungi,” Journal of Chemotherapy, vol. 15, no. 2, pp. 36–47, 2003.[6] F. J. Pastor and J. Guarro, “Alternaria infections: laboratorydiagnosis and relevant clinical features,” Clinical Microbiologyand Infection, vol. 14, no. 8, pp. 734–746, 2008.[7] P. C. Iwen, M. E. Rupp, and S. H. Hinrichs, “Invasive moldsinusitis: 17 cases in immunocompromised patients and reviewof the literature,” Clinical Infectious Diseases, vol. 24, no. 6, pp.1178–1184, 1997.[8] V. A. Morrison and D. J. Weisdorf, “Alternaria: a sinonasalpathogen of immunocompromised hosts,” Clinical InfectiousDiseases, vol. 16, no. 2, pp. 265–270, 1993.[9] S. E. M. Vermeire, H. de Jonge, K. Lagrou, and D. R. J. Kuypers,“Cutaneous phaeohyphomycosis in renal allograft recipients:report of 2 cases and review of the literature,” DiagnosticMicrobiology and Infectious Disease, vol. 68, no. 2, pp. 177–180,2010.[10] E. Hipolito, E. Faria, A. F. Alves et al., “Alternaria infectoriabrain abscess in a child with chronic granulomatous disease,”European Journal of Clinical Microbiology and Infectious Dis-eases, vol. 28, no. 4, pp. 377–380, 2009.[11] A. Angel-Moreno, A. France´s, J. M. Granado, and J. L. Pe´rez-Arellano, “Ventriculoperitoneal shunt infection by Candidaglabrata in an adult,” Journal of Infection, vol. 41, no. 2, pp. 178–179, 2000.[12] A.-M. Korinek, L. Fulla-Oller, A.-L. Boch, J.-L. Golmard,B. Hadiji, and L. Puybasset, “Morbidity of ventricular cere-brospinal fluid shunt surgery in adults: an 8-year study,” Neu-rosurgery, vol. 68, no. 4, pp. 985–994, 2011.[13] H. M. Hagman, E. G. Madnick, A. N. D’Agostino et al.,“Hyphal forms in the central nervous system of patients withcoccidioidomycosis,” Clinical Infectious Diseases, vol. 30, no. 2,pp. 349–355, 2000.Submit your manuscripts athttp://www.hindawi.comStem CellsInternationalHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014MEDIATORSINFLAMMATIONofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Behavioural NeurologyEndocrinologyInternational Journal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Disease MarkersHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014BioMed Research InternationalOncologyJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Oxidative Medicine and Cellular LongevityHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014PPAR ResearchThe Scientific World JournalHindawi Publishing Corporation http://www.hindawi.com Volume 2014Immunology ResearchHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Journal ofObesityJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014 Computational and  Mathematical Methods in MedicineOphthalmologyJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Diabetes ResearchJournal ofHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Hindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Research and TreatmentAIDSHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Gastroenterology Research and PracticeHindawi Publishing Corporationhttp://www.hindawi.com Volume 2014Parkinson’s DiseaseEvidence-Based Complementary and Alternative MedicineVolume 2014Hindawi Publishing Corporationhttp://www.hindawi.com

Chronic leptomeningitis and spinal intradural mass secondary to Alternaria infection in a patient with ventriculoperitoneal shunt

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Chronic leptomeningitis and spinal intradural mass secondary to Alternaria infection in a patient with ventriculoperitoneal shunt

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