A Dorr

A Parra

A Zwan van der

AK Vellimana

AM Sirevaag

AP Pathak

BH Han

BP Chugh

C Baltes

CL Lin

D Mayerich

DA Tata

DL Korol

DR Thal

E Milner

EZL Cummins

FH Linn

G Figueiredo

GA Porter

GY Gan

H Hashimoto

H Meng

H Sheng

HM Duvernoy

HR Weiss

J Cahill

J Hekmatpanah

JE Levasseur

JM Krum

JM Lawrence

K Maeda

M Brinkley

M Eriksdotter-Nilsson

M Sabri

M Shiba

MJ McGirt

MR Hasan

N Jahrling

NF Kassell

P Coyle

PS Tsai

R Mostany

RD Broadwell

RL Macdonald

RLM Zhang

RM Pluta

S Franciosi

S Ghanavati

S Heinzer

S Xue

SJ Schambach

SZ Carmeliet

T Minamikawa

Y Li

Y Miyoshi

Scientific Reports

English

AbstractVarious techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high viscosity, rapid solidification, and its impact on immunohistochemical analysis. To overcome these limitations, we developed a novel technique for assessing cerebral vasospasm using cerebrovascular perfusion with ROX, SE (5-Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye. We found that ROX SE perfusion achieves excellent delineation of the cerebral vasculature, was qualitatively and quantitatively superior to India ink-gelatin casting for the assessment of cerebral vasospasm, permits outstanding immunohistochemical examination of non-vasospasm components of secondary brain injury, and is a more efficient and cost-effective experimental technique. ROX SE perfusion is therefore a novel and highly useful technique for studying cerebrovascular pathology following experimental SAH.</jats:p

Diane J. Aum

Ananth K. Vellimana

Itender Singh

Eric Milner

James W. Nelson

Byung Hee Han

Gregory J. Zipfel

Crossref

A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage

Aum, Diane J

Vellimana, Ananth K

Singh, Itender

Milner, Eric

Nelson, James W

Han, Byung Hee

Zipfel, Gregory J

Digital Commons@Becker

https://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=7112&amp;context=open_access_pubs

A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage

Abstract

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Digital Commons@Becker