Alcala, Marissa

Alvey, Faculty Advisor, Richard

Beagles, Alecia

Bollivar, Faculty Advisor, David

Marchi, Caroline

Ziomek, Samantha

English

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Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 2017, 28th Annual JWP ConferenceApr 8th, 9:00 AM - 10:00 AMThe Isolation, Purification, and Characterization ofFourteen Mycobacterium SmegmatisBacteriophagesMarissa AlcalaIllinois Wesleyan UniversityAlecia BeaglesIllinois Wesleyan UniversityCaroline MarchiIllinois Wesleyan UniversitySamantha ZiomekIllinois Wesleyan UniversityRichard Alvey, Faculty AdvisorIllinois Wesleyan UniversitySee next page for additional authorsFollow this and additional works at: http://digitalcommons.iwu.edu/jwprcPart of the Biology CommonsThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at The Ames Library at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Alcala, Marissa; Beagles, Alecia; Marchi, Caroline; Ziomek, Samantha; Alvey, Faculty Advisor, Richard; and Bollivar,Faculty Advisor, David, "The Isolation, Purification, and Characterization of Fourteen Mycobacterium SmegmatisBacteriophages" (2017). John Wesley Powell Student Research Conference. 9.http://digitalcommons.iwu.edu/jwprc/2017/posters/9Presenter InformationMarissa Alcala; Alecia Beagles; Caroline Marchi; Samantha Ziomek; Richard Alvey, Faculty Advisor; andDavid Bollivar, Faculty AdvisorThis event is available at Digital Commons @ IWU: http://digitalcommons.iwu.edu/jwprc/2017/posters/9THE JOHN WESLEY POWELL STUDENT RESEARCH CONFERENCE – APRIL 2017      Poster Presentation  P1   THE ISOLATION, PURIFICATION, AND CHARACTERIZATION OF FOURTEEN MYCOBACTERIUM SMEGMATIS BACTERIOPHAGES   Marissa Alcala, Alecia Beagles, Caroline Marchi, Samantha Ziomek  and Richard Alvey* and David Bollivar* Biology Department, Illinois Wesleyan University    The overall goal of the SEA-PHAGES program is to utilize microbiology and bioinformatic techniques to study the characteristics of discovered bacteriophages. The collected data will contribute to the growing database of phage genomic information. Since bacteriophages are estimated to be the most numerous biological entities on earth, understanding the diversity of these entities is of significant biological interest. Bacteriophages from gathered soil samples were isolated utilizing the host Mycobacterium smegmatis, mc2155, through direct and enrichment procedures. During the Fall of 2016, fourteen M. smegmatis bacteriophages were isolated and streaked for purification. The fourteen phages were initially characterized by their plaque size and clarity, and their ability to form a lysogen. True lysogens were formed from nine of the fourteen isolated bacteriophages. Further analysis through electron microscopy lead to the determination of tail length and capsid size. Tail lengths ranged from 73 nm to 344 nm, and capsid diameters ranged from 60 nm to 95 nm. Electron microscopic analysis also allowed for the categorization of the fourteen bacteriophages into three classes: siphoviridae, myoviridae, and podoviridae. Twelve of the fourteen phages were classified as siphoviridae and two were classified as myoviridae. Specific phages, Squint, isolated by Alecia Beagles, and Tesla, isolated by Colin Page, were further analyzed because of their unique qualities highlighted during the isolation and characterization processes. The discovery of these fourteen novel bacteriophages is a step towards a greater understanding of phage diversity.     

The Isolation, Purification, and Characterization of Fourteen Mycobacterium Smegmatis Bacteriophages

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The Isolation, Purification, and Characterization of Fourteen Mycobacterium Smegmatis Bacteriophages

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