Balser, Faculty Advisor, Elizabeth J.

Butler, Kimberlee M.

Whitehill, Elizabeth A. G.

English

Digital Commons @ Illinois Wesleyan University

Illinois Wesleyan UniversityDigital Commons @ IWUJohn Wesley Powell Student ResearchConference 2004, 15th Annual JWP ConferenceApr 17th, 9:00 AM - 10:00 AMDetermining the Best Protocol for Raising Larvaeof the Sea Urchin Eucidaris TribuloidesElizabeth A. G. WhitehillIllinois Wesleyan UniversityKimberlee M. ButlerIllinois Wesleyan UniversityElizabeth J. Balser, Faculty AdvisorIllinois Wesleyan UniversityFollow this and additional works at: http://digitalcommons.iwu.edu/jwprcThis Event is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and FacultyDevelopment, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU bythe faculty at Illinois Wesleyan University. For more information, please contact digitalcommons@iwu.edu.©Copyright is owned by the author of this document.Whitehill, Elizabeth A. G.; Butler, Kimberlee M.; and Balser, Faculty Advisor, Elizabeth J., "Determining the Best Protocolfor Raising Larvae of the Sea Urchin Eucidaris Tribuloides" (2004). John Wesley Powell Student Research Conference. 10.http://digitalcommons.iwu.edu/jwprc/2004/posters/10THE JOHN WESLEY POWELL STUDENT RESEARCH CONFERENCE - APRIL 2004 Poster Presentation P53 DETERMINING THE BEST PROTOCOL FOR RAISING LARVAE OF THE SEA URCHIN EVe/DARIS TRIBULO/DES Elizabeth A. G. Whitehill, Kimberlee M. Butler and Elizabeth J. Balser* Department of Biology, Illinois Wesleyan University Previous studies (Balser, 1 998, 2003 ; Eaves and Palmer, 2003) have shown that developmental stages (larvae) of sea stars and other echinoderms are capable of producing new larvae by cloning. One hypothesis emerging from the collected works on larval cloning is that this phenomenon is a pleisomorphic character for the Echinodermata. The pencil urchin, Eucidaris tribuloides, embodies several characters, such as the arrangement of skeletal plates, the number of tube feet per plate, and the morphology of the feeding teeth, that are considered primitive for the Echinoidea (the class to which sea urchins belong). Because E. tribuloides may represent an ancient lineage within the echinoids, we predict that the feeding larva of this species is capable of asexual reproduction during the larval phase. In further pursuit of this hypothesis, we attempted to determine the best protocol for culturing larvae of this species in the laboratory. Adult specimens of E. tribuloides were spawned, and fertilized eggs were raised either in stirred or unstirred filtered seawater. All larvae were exposed to a photoperiod of 12L: 1 2D .  Some animals kept in unstirred water were raised at room temperature (23-24°C), while others were placed in an incubator at 26°C. While all animals were fed the alga Rhodomonas lens ( 1 07 cells per ml), some received a supplemental diet of EZ Larva™ (approximately 1 05 particles per ml). We found that the larvae grew best at room temperature in moving water when they were fed only R. lens. 

Determining the Best Protocol for Raising Larvae of the Sea Urchin \u3cem\u3eEucidaris Tribuloides\u3c/em\u3e

https://digitalcommons.iwu.edu/cgi/viewcontent.cgi?article=1889&amp;context=jwprc

Determining the Best Protocol for Raising Larvae of the Sea Urchin \u3cem\u3eEucidaris Tribuloides\u3c/em\u3e

Abstract

Similar works

Full text

Available Versions

Digital Commons @ Illinois Wesleyan University