Catellani, Silvia

Durando, Paolo

Fenoglio, Daniela

Ferrera, Alessandra

Gasperini, Roberto

Parodi, Alessia

Poggi, Alessandro

Zocchi, Maria Raffaella

English

PubMed

Maria Raffaella Zocchi

Daniela Fenoglio

Alessia Parodi

Alessandra Ferrera

Paolo Durando

Roberto Gasperini

Silvia Catellani

Alessandro Poggi

Crossref

POSTER PRESENTATION Open Access
Expansion of vdelta1 T lymphocytes reactive to
c. albicans IN HIV-1 infected patients: effect of
influenza virus vaccine
Maria Raffaella Zocchi1*, Daniela Fenoglio2, Alessia Parodi2, Alessandra Ferrera2, Paolo Durando3,
Roberto Gasperini3, Silvia Catellani4, Alessandro Poggi5
From 16th International Symposium on HIV and Emerging Infectious Diseases
Marseille, France. 24-26 March 2010
Background
It is known that the circulating Vdelta2 T cell subset
respond to mycobacteria and certain viruses, while the
Vdelta1 subset is resident in the mucosal-asssociated
lymphoid tissue and participate in the immunity against
intracellular microrganisms. We reported that in HIV-1
infected patients circulating Vdelta1 T lymphocytes are
increased; in vitro, these cells can proliferate in response
to Candida albicans. We analysed the effects of influenza
virus vaccination on the function of this T cell subset in
HIV-1 infected patients and healthy donors.
Methods
We analysed the effects of influenza virus vaccination on
the function of Vdelta1 and Vdelta2 T cell subsets in
HIV-1 infected patients and healthy donors. Cells were
isolated from blood samples obtained before and after
30 or 90 days after vaccination. Proliferation to C.albi-
cans and to hemoagglutimnin (HA) was assessed by thy-
midine uptake after 7 days of stimulation.
Results
First, we confirmed that the Vdelta1 T cell subset is
expanded in HIV-1 infected patients (absolute number
of cells/microliter range 28-30 in HIV-1 patients vs. 8-
12 in healthy donors). On day 90 after vaccination the
number of Vdelta1 T cells significantly increased in
HIV-1 patients (59 in the group A, 48 in the group B).
Interestingly, upon influenza vaccination an increase in
proliferation of Vdelta1 T cells to C. albicans was
observed in HIV-1 patients, at variance with healthy
donors, on day 30 and day 90. A specific cellular
response to HA was detectable in HIV-1 patients only
on day 90 post-vaccination without MF59 adjuvant, but
it was observed on day 30, as in seronegative subjects,
when MF59-vaccine was used.
Discussion
We suggest that in HIV-1 infected patients, a population
of Vdelta1 T lymphocytes reactive to C. albicans is pre-
sent in vivo; upon challenge with influenza virus vaccine
this population receives an activation signal possibly
mediated by cytokines triggered by the HA antigen
itself.
Author details
1Scientific Institute San Raffaele, Department of Immunology, Milan, Italy.
2CEBR, University of Genoa, Genoa, Italy. 3Department of Health Science, San
Martino Hospital, Genoa, Italy. 4Department of Oncohematology, Universiti of
Genoa, Genoa, Italy. 5National Institute for Cancer Research, Unit of
Molecular Oncology and Angiogenesis, Genoa, Italy.
Published: 11 May 2010
doi:10.1186/1742-4690-7-S1-P25
Cite this article as: Zocchi et al.: Expansion of vdelta1 T lymphocytes
reactive to c. albicans IN HIV-1 infected patients: effect of influenza
virus vaccine. Retrovirology 2010 7(Suppl 1):P25.
* Correspondence: zocchi.maria@hsr.it
1Scientific Institute San Raffaele, Department of Immunology, Milan, Italy
Zocchi et al. Retrovirology 2010, 7(Suppl 1):P25
http://www.retrovirology.com/content/7/S1/P25
© 2010 Zocchi et al; licensee BioMed Central Ltd.


Expansion of vdelta1 T lymphocytes reactive to c. albicans IN HIV-1 infected patients: effect of influenza virus vaccine

Expansion of vdelta1 T lymphocytes reactive to c. albicans IN HIV-1 infected patients: effect of influenza virus vaccine

Abstract

Similar works

Full text

Available Versions

Crossref

Springer - Publisher Connector

Directory of Open Access Journals

Springer - Publisher Connector