Boerrigter, Guido

Burnett, John C

Cataliotti, Alessandro

Costello-Boerrigter, Lisa

Heublein, Denise

Lahr, Brian

Macheret, Fima

McKie, Paul

Rodeheffer, Richard

Sandberg, Sharon

BMC Pharmacology

English

PubMed

Fima Macheret

Denise Heublein

Brian Lahr

Lisa Costello-Boerrigter

Guido Boerrigter

Paul McKie

Sharon Sandberg

Richard Rodeheffer

Alessandro Cataliotti

John C Burnett

Springer - Publisher Connector

Pro-B-Type natriuretic peptide: a novel, specific biomarker for detection of left ventricular dysfunction in the general community

BioMed Central
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BMC Pharmacology
Open AccessPoster presentation
Pro-B-Type natriuretic peptide: a novel, specific biomarker for 
detection of left ventricular dysfunction in the general community
Fima Macheret*, Denise Heublein, Brian Lahr, Lisa Costello-Boerrigter, 
Guido Boerrigter, Paul McKie, Sharon Sandberg, Richard Rodeheffer, 
Alessandro Cataliotti and John C Burnett Jr
Address: Cardiorenal Research Laboratory, Division of Cardiovascular Diseases, Mayo Clinic, Rochester, MN USA
Email: Fima Macheret* - macheret.fima@mayo.edu
* Corresponding author    
Background
Pro-B-Type Natriuretic Peptide (ProBNP), from which
biologically active BNP and inactive amino-terminal BNP
(NT-proBNP) are processed, has recently been reported to
be elevated in patients with heart failure (HF). It remains
to be shown in a large cohort study whether ProBNP cir-
culates in normals, what factors influence ProBNP, and
whether or not it is a sensitive biomarker for left ventricu-
lar (LV) dysfunction.
We test whether a novel assay (BioRad) for ProBNP will be
specific for ProBNP and not cross react with other forms
of BNP. Furthermore, we hypothesize that ProBNP levels
will be most determined by age and gender and that
ProBNP will be a sensitive and specific biomarker for LV
dysfunction in the general community.
Methods
We used a large community cohort (n = 2009; age => 45)
from Olmsted County, MN. All subjects had detailed
echocardiography, clinical phenotyping, and assessment
of comorbidities. ProBNP was measured using BioRad
assay. Univariable and multivariable analysis were used to
determine which clinical factors best determine ProBNP
levels in all subjects. ROC curves were generated by com-
paring ProBNP to 2-D Doppler Echocardiography in
detecting LV dysfunction. Assay specificity was assessed by
adding 8 different forms of BNP to normal human
plasma.
Results
ProBNP was present in normals (median male = 10 pg/
ml, female = 20; p < .001) and elevated in those with
EF<40% (median male = 195, female = 188; n = 36). Gen-
der and age (Spearman > .3, p < .01) were strong predic-
tors of Pro-BNP. ProBNP was a sensitive and specific
marker for LV dysfunction based on ROC curves
(AUC=.86). Finally, the BioRad assay did not cross react
with any BNP forms that we added to normal human
plasma.
Conclusion
Using a novel ProBNP assay, we are the first to establish
normal ProBNP levels in a large general adult population,
suggesting that the normal human heart secretes ProBNP.
We conclude that age and gender should be considered in
interpretation of ProBNP values, similar to BNP and NT-
proBNP. Importantly, in the entire population of ran-
domly selected adults in the general community, ProBNP
was a sensitive and specific biomarker for the detection of
LV dysfunction. Thus, these studies establish the utility of
ProBNP as a biomarker for HF, and we suggest further
studies to better establish the biologic and pathophysio-
logic significance of ProBNP in humans.
from 4th International Conference of cGMP Generators, Effectors and Therapeutic Implications
Regensburg, Germany. 19–21 June 2009
Published: 11 August 2009
BMC Pharmacology 2009, 9(Suppl 1):P44 doi:10.1186/1471-2210-9-S1-P44
<supplement> <title> <p>4th International Conference of cGMP Generators, Effectors and Therapeutic Implications</p> </title> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1471-2210-9-S1-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2210-9-S1-info.pdf</url> </supplement>
This abstract is available from: http://www.biomedcentral.com/1471-2210/9/S1/P44
© 2009 Macheret et al; licensee BioMed Central Ltd. 


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Pro-B-Type natriuretic peptide: a novel, specific biomarker for detection of left ventricular dysfunction in the general community

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