AbdEl-Monem El-Sharkawy

AM El-Sharkawy

Gary Gerstenblith

Gurusher S Panjrath

Kerry Stewart

M Schär

Michael Schär

Paul A Bottomley

Robert G Weiss

Steven P Schulman

English

PubMed

Panjrath Gurusher S

Schär Michael

El-Sharkawy AbdEl-Monem

Schulman Steven P

Stewart Kerry

Gerstenblith Gary

Bottomley Paul A

Weiss Robert G

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Journal of Cardiovascular Magnetic Resonance

Skeletal muscle high energy phosphate metabolism in patients with obesity and impaired fasting blood glucose

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POSTER PRESENTATION Open AccessSkeletal muscle high energy phosphatemetabolism in patients with obesity and impairedfasting blood glucoseGurusher S Panjrath1*, Michael Schär2, AbdEl-Monem El-Sharkawy2, Steven P Schulman1, Kerry Stewart1,Gary Gerstenblith1, Paul A Bottomley2, Robert G Weiss1,2From 15th Annual SCMR Scientific SessionsOrlando, FL, USA. 2-5 February 2012SummaryTo determine whether SM CK energy metabolism isimpaired in obesity-associated IFG SM at rest andwhether there is any relationship between SM CKenergy metabolism and exercise capacity in thesepatients.BackgroundSkeletal muscle (SM) is the major site of glucose con-sumption and the creatine kinase (CK) reaction is theprimary energy reserve in SM, providing additional ATPduring ischemia and exercise. Although obesity-asso-ciated impaired fasting glucose (O+IFG) impairs SMglucose uptake and energy metabolism, it is not clearwhether CK energy metabolism is also altered in O+IFG.MethodsMethod: Ten obese participants with IFG (O+IFG),defined as BMI>30 and fasting glucose ≥100 mg/dl orHbA1c ≥ 5.7, twelve obese participants without IFG (O-IFG), and five non-obese subjects with normal fastingglucose underwent calf muscle 31P MRS with saturationtransfer measures of CK metabolism at rest on a Philips3T MRI/MRS system. The CK pseudo-first-order rateconstant, k (sec-1) was calculated using TRiST and rela-tive high-energy phosphate levels as previously described[1,2]. O-IFG and O+IFG subjects also underwent tread-mill exercise testing for determination of maximal exer-cise time and peak oxygen consumption. Groupcomparison and correlations within each group wereperformed using XLSTAT package.ResultsResults: Baseline demographics, anthropometric andmetabolic measures, and 31P MRS results are detailedin Table 1. The mean CK pseudo-first order rate con-stant, k and mean PCr/ATP ratios were similar amongall participant groups. There were no significant correla-tions between k and other exercise, metabolic or anthro-pometric parameters.ConclusionsWe believe this is the first report on CK kinetics in rest-ing SM in obese subjects with impaired fasting glucose.The findings indicate that intracellular ATP CK kineticsare preserved at rest in these subjects compared withobese subjects with normal glucose or healthy subjects.ATP kinetics via CK of resting SM do not appear to beassociated with indices of glycemic control or exercisecapacity. Thus, SM CK high-energy phosphate levelsand ATP kinetics appear to be normal at rest in obesesubjects with and without IFG. The response of CKmetabolism during exercise in obese subjects is yet tobe determined.FundingNIH HL61912 and Clarence Doodeman Endowment.Author details1Medicine/Cardiology, The Johns Hopkins University School of Medicine,Baltimore, MD, USA. 2Radiology, The Johns Hopkins University School ofMedicine, Baltimore, MD, USA.Published: 1 February 20121Medicine/Cardiology, The Johns Hopkins University School of Medicine,Baltimore, MD, USAFull list of author information is available at the end of the articlePanjrath et al. Journal of Cardiovascular Magnetic Resonance 2012, 14(Suppl 1):P74http://www.jcmr-online.com/content/14/S1/P74© 2012 Panjrath et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.References1. Schär M, El-Sharkawy AM, Weiss RG, Bottomley PA: Triple repetition timesaturation transfer (TRiST) 31P spectroscopy for measuring humancreatine kinase reaction kinetics. Magn Reson Med 2010, 63(6):1493-501.2. El-Sharkawy AM, Schär M, Ouwerkerk R, Weiss RG, Bottomley PA:Quantitative cardiac 31P spectroscopy at 3 Tesla using adiabatic pulses.Magn Reson Med 2009, 61(4):785-95.doi:10.1186/1532-429X-14-S1-P74Cite this article as: Panjrath et al.: Skeletal muscle high energyphosphate metabolism in patients with obesity and impaired fastingblood glucose. Journal of Cardiovascular Magnetic Resonance 2012 14(Suppl 1):P74.Submit your next manuscript to BioMed Centraland take full advantage of: • Convenient online submission• Thorough peer review• No space constraints or color figure charges• Immediate publication on acceptance• Inclusion in PubMed, CAS, Scopus and Google Scholar• Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submitTable 1Healthy (n=5) O-IFG (n=12) O+IFG (n=10)Age (yrs) 37.8 ± 8.7 52.4 ± 8.1* 52.8 ± 7.7*Male % 100 25 20BMI 24.0 ± 3.0 35.4 ± 5.1* 34.5 ± 4.4*Plasma glucose (mg/dl) - 90.8 ± 5.6 114.4 ±14.2¥HbA1c - 5.2 ± 0.3 5.6 ± 0.5§Metabolic syndrome % - 50 40VO2 ml/kg/min - 25.7 ± 4.4 24.2 ± 4.3ETT(min) 914.7 ± 288 809.6 ± 232PCr/ATP 6.27 ± 0.52 6.52 ± 0.59 6.85 ± 0.49k (s-1) 0.29 ± 0.03 0.28 ± 0.03 0.26 ± 0.04Mean±standard deviation. BMI- body mass index; HbA1c- Glycosylatedhemoglobin; VO2- peak oxygen consumption; ETT- Treadmill Exercise Time;PCr/ATP- phosphocreatine/adenosine triphosphate ratio; k- CK reactionpseudo-first order rate constant. * p< 0.01 compared to healthy; § p < 0.05and ¥ p<0.001 compared to O-IFG.Panjrath et al. Journal of Cardiovascular Magnetic Resonance 2012, 14(Suppl 1):P74http://www.jcmr-online.com/content/14/S1/P74Page 2 of 2

Cite this article as: Panjrath et al.: Skeletal muscle high energy phosphate metabolism in patients with obesity and impaired fasting blood glucose.

Triple repetition time saturation transfer (TRiST) 31P spectroscopy for measuring human creatine kinase reaction kinetics. Magn Reson Med

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Skeletal muscle high energy phosphate metabolism in patients with obesity and impaired fasting blood glucose

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