Analysis of the Peptidoglycan Hydrolase Complement of Lactobacillus casei and Characterization of the Major γ-D-Glutamyl-L-Lysyl-Endopeptidase

A Maze; A Seth; A Steen; Alain Guillot; B Corthesy; B Foligne; B Sperandio; C Bauerl; C Chaput; C Huard; E Bernard; E Macho-Fernandez; EM Bryan; F Yan; I Claes; Indranil Biswas; Ingmar J. J. Claes; J Asong; J Beganovic; J Humann; J Humann; J Rappsilber; J Sambrook; JC Whisstock; JM Lambert; Jos Vanderleyden; K Shida; Krzysztof Regulski; L Fontaine; M Meyrand; M Nagaoka; M-P Chapot-Chartier; Marie-Pierre Chapot-Chartier; Mickael Meyrand; O Emanuelsson; P Courtin; P Veiga; Pascal Courtin; Pascal Hols; PG de Ruyter; RD Finn; S Layec; S Lebeer; Sarah Lebeer; SE Girardin; T Rochat; TW Aukrust; W Vollmer; W Vollmer; WJ Dower; Y Ishihama

Analysis of the Peptidoglycan Hydrolase Complement of Lactobacillus casei and Characterization of the Major γ-D-Glutamyl-L-Lysyl-Endopeptidase

Abstract

Peptidoglycan (PG) is the major component of Gram positive bacteria cell wall and is essential for bacterial integrity and shape. Bacteria synthesize PG hydrolases (PGHs) which are able to cleave bonds in their own PG and play major roles in PG remodelling required for bacterial growth and division. Our aim was to identify the main PGHs in Lactobacillus casei BL23, a lactic acid bacterium with probiotic properties