Detailed gel images of the differential spots identified by mass spectrometry and database search. Magnification of the 2D gel-areas corresponding to the 19 differentially expressed spots identified by mass spectrometry and database search. Detailed images for each of the 6 replicates analyzed (3 corresponding to the ESPs of E. caproni adults obtained from rats, and 3 from those isolated from mice) are shown. Spot numbers refer to gel image in Fig. 2. (PPTX 4834 kb

Alba Cortés

Carla Muñoz-Antolí

J. Guillermo Esteban

Javier Sotillo

María Trelis

Rafael Toledo

Parasites & Vectors

PubMed

Springer - Publisher Connector

Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

Background: Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments.



Methods: To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed.



Results: A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host.



Conclusions: The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival

Cortés, Alba

Sotillo-Gallego, Javier

Muñoz-Antolí, Carla

Trelis, Maria

Esteban, J. Guillermo

Toledo, Rafael

ResearchOnline@JCU

Crossref

Identification details of differentially expressed proteins. Description of data: Details of the identification of differentially expressed proteins using X!Tandem and MS-GF+ search engines on the Echinostoma caproni genome and transcriptome databases and BLASTp analysis against NCBInr protein database. Spot numbers refer to gel image in Fig. 2. (DOCX 19 kb

Alba Cortés (134065)

Javier Sotillo (134069)

Carla Muñoz-Antolí (3536486)

María Trelis (134059)

J. Esteban (3536489)

Rafael Toledo (134093)

The Francis Crick Institute

Additional file 3: of Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

Biological process-based classification of proteins overexpressed in mice. Significantly overexpressed proteins in the excretory/secretory products of Echinostoma caproni adults obtained from mice, classified according to their Gene Ontology (GO)-predicted biological process. Pie chart represents the number of proteins assigned to each GO category (biological process, level 4). Proteins included in each category are listed below the graph. (PPTX 118 kb

Additional file 4: of Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

Molecular function-based classification of proteins overexpressed in mice. Significantly overexpressed proteins in the excretory/secretory products of Echinostoma caproni adults obtained from mice, classified according to their Gene Ontology (GO)-predicted molecular function. Pie chart represents the number of proteins assigned to each GO category (molecular function, level 3). Proteins included in each category are listed below the graph. (PPTX 77 kb

Additional file 5: of Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

Additional file 1: of Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

ResearchOnline at James Cook University

English

https://researchonline.jcu.edu.au/43698/1/43698%20Cortes%20et%20al%202016.pdf

Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

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Springer - Publisher Connector

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The Francis Crick Institute

The Francis Crick Institute

Springer - Publisher Connector

The Francis Crick Institute

The Francis Crick Institute

ResearchOnline at James Cook University