Annie Marc

Caroline Ferrari

Cécile Nouvel

Emmanuel Guedon

Eric Olmos

Frédérique Balandras

Isabelle Chevalot

Jean-Luc Six

Jérôme Babin

Mukesh Gupta

Nguyen Tran

BMC Proceedings

PubMed

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Adhesion and colonization of mesenchymal stem cells on polylactide or PLCL fibers dedicated for tissue engineering

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POSTER PRESENTATION Open Access
Adhesion and colonization of mesenchymal stem
cells on polylactide or PLCL fibers dedicated for
tissue engineering
Frédérique Balandras1, Caroline Ferrari1, Eric Olmos1, Mukesh Gupta2, Cécile Nouvel2, Jérôme Babin2, Jean-Luc Six2,
Nguyen Tran3, Isabelle Chevalot1, Emmanuel Guedon1*, Annie Marc1
From 23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health
Lille, France. 23-26 June 2013
Background
Tissue engineering covers a broad range of applications
dedicated to the repair or the replacement of part or
whole tissue such as blood vessels, bones, cartilages, liga-
ments, etc [1]. Practically, a bio substitute, made with
cells cultivated on scaffold, is needed. Mesenchymal stem
cells (MSC) are generally the most suitable cells for such
application since they are self-renewable with a great
potential for differentiation and immuno suppression [2].
However, materials used for bio functional scaffold
synthesis have to meet several criteria, such as biocom-
patibility and biodegradability. Thus, the aim of the study
was to screen several biopolymers differing in their com-
position for their capability to promote adhesion and
growth of MSC.
Materials and methods
Porcine MSC were cultivated in a-MEM supplemented
with 10% serum and FGF2. For cell adhesion experi-
ments, 6(co)polymers (Table 1) were synthesized and
tested.
Fibres of polymers were electrospun on 4 cm2 cover
glasses. Briefly, the polymer solutions are introduced into
a syringe with various flow rates and an electrical field is
applied, resulting in the formation of a polymer jet on
cover glasses or on any surfaces. Then, cover glasses
were put onto 6 wells plate before to be seeded with
MSC. Then, cell adhesion and colonization of polymer
fibres were monitored by microscopy and counted using
Guava Viacount assay after trypsine treatment as already
described [3].
Results
With the aim of studying and identifying new materials
dedicated to scaffold manufacturing for tissue ingineer-
ing, MSC were cultivated on various (co) polymers.
These polymers, made with lactic acid (L and/or D) and/
or caprolactone (blue bars; MKG 58, 64, 70, 71 and 74)
in comparison with a commercial PLCL (red bars), were
electrospun on cover glasses in order to functionalize
them. Then MSC were cultivated on theses functiona-
lized cover glasses at two initial cell seeding (10 000 and
60 000 cells) during 200 hours (Figure 1).
Whatever the polymer used and the initial cell seed-
ing, cells were able to adhere and to colonize fibres. A
cell multiplication factor ranging from 6.5 to 22 was
measured after 200 hours of culture depending on the
polymer composition and the initial seeding. However,
compared to the commercial PLCL, the total cell num-
ber was strongly increased with MKG 71 (21 and 50%),
MKG 74 (34 and 34%) and MKG 58 (15 and 40%)
whereas a moderate growth was observed with MKG 64
(9 and 30%) at an initial seeding of 10 000 and 60 000
cells respectively. MKG 70 did not improve the cell
growth compared to the commercial polymer (> 5% for
both seeding).
Conclusion
In this study, porcine MSC were cultivated on various
(co)polymers made with lactic acid (L and/or D) and/or
caprolactone. Our results demonstrated that composition
of these (co)polymers strongly influences MSC growth
and colonization. Indeed, polymers such as MKG 58, 71
* Correspondence: emmanuel.guedon@univ-lorraine.fr
1CNRS, Laboratoire Réactions et Génie des Procédés, UMR 7274, Université
de Lorraine-ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518
Vandoeuvre-lès-Nancy Cedex, France
Full list of author information is available at the end of the article
Balandras et al. BMC Proceedings 2013, 7(Suppl 6):P53
http://www.biomedcentral.com/1753-6561/7/S6/P553
© 2013 Balandras et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
and 74 appeared to promote MSC growth contrary to
other polymers tested, i;e MKG 64 and MKG 70, com-
pared to the commercial one. Therefore, MKG 58, 71
and 74 could be favoured for further scaffold synthesis.
Authors’ details
1CNRS, Laboratoire Réactions et Génie des Procédés, UMR 7274, Université
de Lorraine-ENSAIA, 2 avenue de la forêt de Haye, TSA 40602, F-54518
Vandoeuvre-lès-Nancy Cedex, France. 2CNRS, Laboratoire de Chimie Physique
Macromoléculaire, FRE 3564, Université de Lorraine-ENSIC, 1 rue Grandville,
54000 Nancy Cedex, France. 3École de Chirurgie, Faculté de Médecine,
Université de Lorraine, F-54500 -Vandœuvre-lès-Nancy, France.
Published: 4 December 2013
References
1. Caplan AI: Adult mesenchymal stem cells for tissue engineering versus
regenerative medicine. J Cell Physio 2007, 213:341-347.
2. Chamberlain G, Fox J, Ashton B, Middleton J: Concise review:
Mesenchymal stem cells: Their phenotype, differentiation capacity,
immunological features, and potential for homing. Stem Cells 2007,
25:2739-2749.
3. Ferrari C, Balandras F, Guedon E, Olmos E, Chevalot I, Marc A: Limiting cell
aggregation during mesenchymal stem cell expansion on microcarriers.
Biotechnol Prog 2012, 28:780-787.
doi:10.1186/1753-6561-7-S6-P53
Cite this article as: Balandras et al.: Adhesion and colonization of
mesenchymal stem cells on polylactide or PLCL fibers dedicated for
tissue engineering. BMC Proceedings 2013 7(Suppl 6):P53.
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Table 1 Composition of co-polymers used in this study
Commercial PLCL 70% L-LA 30% CL
MKG58 70% D, L-LA 30% CL
MKG64 - 100% CL
MKG70 50%L-LA 50% CL
MKG71 100%D,L-LA -
MKG74 100%L-LA -
LA: lactic acid; CL: ε-caprolactone
Figure 1 Quantitative evaluation of MSC growth on poly lactide/caprolactone polymers. Two initial MSC seeding, 10 000 and 60 000 cells,
were carried out. The red stars indicate a significant increase in final cell number compared to the control (commercial PLCL).
Balandras et al. BMC Proceedings 2013, 7(Suppl 6):P53
http://www.biomedcentral.com/1753-6561/7/S6/P553
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Adhesion and colonization of mesenchymal stem cells on polylactide or PLCL fibers dedicated for tissue engineering

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