The Wuerzburg Hybridoma Library against the
Drosophila brain represents a
collection of around 200 monoclonal antibodies
that bind to specific structures in the
Drosophila brain. Here we
describe the immunohistochemical staining
patterns, the Western blot signals of one- and
two-dimensional electrophoretic separation, and
the mass spectrometric characterization of the
target protein candidates recognized by the
monoclonal antibodies aa2 and ab52 from the
library. Analysis of a mutant of a candidate gene
identified the Drosophila homolog
of the Epidermal growth factor receptor Pathway
Substrate clone 15 (Eps15) as the antigen for
these two antibodies