B Demesure

Helena Cvrcková

HM Heybroek

Jana Malá

Jaromir Mikulik

Karel Dolezal

Lucie Szucova

Lukas Spichal

M Strnad

Michal Karady

Miroslav Strnad

Ondrej Novák

Pavlína Máchová

SPO Werbrouck

BMC Proceedings

English

PubMed

Crossref

New cytokinin derivatives – their discovery, development and use for micropropagation of endangered tree species

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ORAL PRESENTATION Open Access
New cytokinin derivatives – their discovery,
development and use for micropropagation
of endangered tree species
Karel Dolezal1*, Jana Malá2, Pavlína Máchová2, Helena Cvrcková2, Michal Karady1, Ondrej Novák1, Lucie Szucova3,
Jaromir Mikulik1, Lukas Spichal3, Miroslav Strnad3
From IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery
Arraial d’Ajuda, Bahia, Brazil. 26 June - 2 July 2011
Elms (Ulmus sp.) are highly valuable trees for their great
strength, tightly twisted grain, and durability, as well as
for their cold and salt tolerance[1]. Wych elm (Ulmus
glabra, Huds.) is a native species in Europe. In the Czech
Republic, it is spread especially in mountain regions.
During the years 1970–80, the second epidemic of Dutch
elm disease destroyed most elm populations in Europe.
The elm trees, which have survived the disease, are cap-
able to maintain a high degree of resistance against the
repeated infection. Micropropagation represents promis-
ing alternative of their vegetative reproduction.
The wild service tree (Sorbus torminalis (L.) Crantz) is
a slow-growing tree reaching maximum height of 20–25
m at around 80–100 years. According to the latest data,
the species is also disappearing from forests of the
Czech Republic and it is considered to be endangered.
On the other hand, the wild service tree is rated as one
of the most valuable hardwoods [2] with a great poten-
tial for wider use in forestry and forest ecology, and also
for its importance in the timber industry.
The in vitro induction of organogenesis, rooting and
acclimatization of above mentioned elm species as well
as wild service tree were optimalized and standartized.
Widely used BAP is an important and affordable cytoki-
nin routinely utilized for its effective stimulatory proper-
ties in micropropagation practice. On the other hand,
BAP may negatively influence the growth, rooting and
acclimatization processes in some crops [3].It is known
that hydroxylated aromatic cytokinins (topolins) are
more resistant to cytokinin oxidase (CKX), more stable
and in some biological systems active at lower concetra-
tions than the isoprenoid CKs. In addition, meta - topo-
lin does not inhibit root formation which is a typical
inconvenient effect of high concentrations of BAP.
These properties might help to enhance the future pro-
ductivity of plant tissue culture industry crops [3,4]. The
development of other new CK derivatives exhibiting
high morphogenetic activity might consequently be of a
great practical importance in plant biotechnology.During
our recent search for naturally occurring aromatic cyto-
kinins (ARCKs) in plants, another new group of endo-
genous BAP derivatives –methoxytopolins – exhibiting
high biological, especially anti-senescence (and surpris-
ingly also anti-cancer) activity, was discovered. Based on
these results, we synthesized several groups of their syn-
thetic analogues that exhibited high activity in three dif-
ferent CK bioassays and showed the ability to activate
cytokinin receptors and/or to inhibit CKX. Best com-
pounds so far (6-(3-hydroxybenzylamino)purine (mT)
and the 6-(3-methoxybenzylamino)purine-9-ribofurano-
side (MeomTR)) were used for retardation of senescence
during multiplication stage of micropropagation of
above mentioned tree species as well as for rooting sup-
port. Subsequently, wide range of endogenous plant hor-
mones (isoprenoid cytokinins, IAA, ethylene) were
quantified (using UPLC-MS/MS and/or GC/FID, respec-
tively) and compared in relation to cytokinin exogen-
ously used.
The results about optimal endogenous plant hormone
concentrations and their dependence on different exo-
genous cytokinin used in the cultivation media, may
improve in-vitro micropropagation efficiency as well as
quality of ex vitro acclimatized plants of above mentioned
* Correspondence: karel.dolezal@upol.cz
1Laboratory of Growth Regulators, Palacký University and Institute of
Experimental Botany AS CR, 783 71 Olomouc-Holice, The Czech Republic
Full list of author information is available at the end of the article
Dolezal et al. BMC Proceedings 2011, 5(Suppl 7):O46
http://www.biomedcentral.com/1753-6561/5/S7/O46
© 2011 Dolezal et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
elm species, wild service tree and possibly also of other
tree species.
This work was supported by the Ministry of Agricul-
ture of the Czech Republic (Mze 0002070202, QI
92A247), Czech Ministry of Education (MSM
6198959216), ED0007/01/01 Centre of the Region Haná
for Biotechnological and Agricultural Research.
Author details
1Laboratory of Growth Regulators, Palacký University and Institute of
Experimental Botany AS CR, 783 71 Olomouc-Holice, The Czech Republic.
2Institute of Forest and Game Management, Strnady 136, 252 02 Jílovišt?,
The Czech Republic. 3Centre of the Region Haná for Biotechnological and
Agricultural Research, Faculty of Science, Palacký University, Šlechtitel? 11, CZ
783 71 Olomouc, Czech Republic.
Published: 13 September 2011
References
1. Heybroek HM, Elgersma DM, Scheffer RJ: Dutch elm diesease - an
ecological accident. Outlook Agric 1982, 11:1-9.
2. Demesure B, Leguerroué B, Lucchi G, Prat D, Petit RJ: Genetic variability of
a scattered temperate forest tree Sorbus torminalis L-(Crantz). Ann. For.
Sci 2000, 57:63-71.
3. Strnad M: The aromatic cytokinins. Physiol. Plant 1997, 101:674-688.
4. Werbrouck SPO, Strnad M, Van Onckelen HA, Debergh PC: Meta-topolin, an
alternative to benzyladenine in tissue culture? Physiol. Plant 1996,
98:291-298.
doi:10.1186/1753-6561-5-S7-O46
Cite this article as: Dolezal et al.: New cytokinin derivatives – their
discovery, development and use for micropropagation of endangered
tree species. BMC Proceedings 2011 5(Suppl 7):O46.
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Page 2 of 2


Debergh PC: Meta-topolin, an alternative to benzyladenine in tissue culture?

RJ: Dutch elm diesease - an ecological accident. Outlook Agric

RJ: Genetic variability of a scattered temperate forest tree Sorbus torminalis L-(Crantz).

Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color ﬁgure charges • Immediate publication on acceptance

The aromatic cytokinins.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3239918

New cytokinin derivatives – their discovery, development and use for micropropagation of endangered tree species

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