Basset, P

Blanc, D

Monecke, S

English

PubMed

P Basset

S Monecke

D Blanc

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BMC Proceedings

DNA microarray analysis reveals variability of accessory genes among isolates recovered during an MRSA outbreak

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POSTER PRESENTATION Open Access
DNA microarray analysis reveals variability of
accessory genes among isolates recovered during
an MRSA outbreak
P Basset1*, S Monecke2,3, D Blanc1
From International Conference on Prevention & Infection Control (ICPIC 2011)
Geneva, Switzerland. 29 June – 2 July 2011
Introduction / objectives
Although MRSA outbreaks might have significant conse-
quences on patients and/or health care resources, few
data are available about the microevolution of a strain
during a single outbreak. In this study, our aim was to
characterize the variability of accessory genes among iso-
lates recovered during a local MRSA outbreak.
Methods
Ten MRSA isolates recovered during a large outbreak
occurring at the Lausanne University Hospital (Switzer-
land) were characterized using a DNA microarray (Sta-
phyType, Alere Technologies, Germany) that targets
approximately 180 genes including several resistance
and virulence genes. Each isolate belonged to the South
German clone (ST-228-SCCmec I) and had been recov-
ered between September 2008 and December 2009.
Results
As expected during the clonal dissemination of a strain,
the 10 isolates shared identical presence/absence for most
of the c.a. 180 genes tested. Nevertheless, variation was
observed for several resistance and virulence genes. These
included the beta lactamase operon genes (blaZ, blaI,
blaR), genes involved in the resistance to Trimethoprim
and Mupirocin (dfrA and mupR, respectively), a gene
encoding unspecific efflux pump conferring resistance to a
variety of antiseptic such as chlorhexidine (qacA), and
genes potentially involved in virulence (lukX, lukY, aur).
This variability affected at least 5 of the ten isolates.
Conclusion
Our results indicate the gain/loss of resistance and viru-
lence genes during a local outbreak, suggesting that the
biological characteristics of the strain might vary through
time.
Disclosure of interest
None declared.
Author details
1Hospital preventive medicine, Centre Hospitalier Universitaire Vaudois,
Lausanne, Switzerland. 2Institute for Medical Microbiology and Hygiene,
Dresden University of Technology, Dresden, Germany. 3Alere Technology,
Jena, Germany.
Published: 29 June 2011
doi:10.1186/1753-6561-5-S6-P178
Cite this article as: Basset et al.: DNA microarray analysis reveals
variability of accessory genes among isolates recovered during an
MRSA outbreak. BMC Proceedings 2011 5(Suppl 6):P178.
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1Hospital preventive medicine, Centre Hospitalier Universitaire Vaudois,
Lausanne, Switzerland
Full list of author information is available at the end of the article
Basset et al. BMC Proceedings 2011, 5(Suppl 6):P178
http://www.biomedcentral.com/1753-6561/5/S6/P178
© 2011 Basset et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


DNA microarray analysis reveals variability of accessory genes among isolates recovered during an MRSA outbreak

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