Bruce, Catherine R

da Silva Dantas, Alessandra

MacCallum, Donna M

Morgan, Brian A

Quinn, Janet

Rodgers, David

Smith, Deborah A

PLoS ONE

English

PubMed

Aberdeen University Research

A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans.

A fission yeast gene (prr1+) that encodes a response regulator implicated in oxidative stress response.

Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator.

Candida albicans response regulator gene SSK1 regulates a subset of genes whose functions are associated with cell wall biosynthesis and adaptation to oxidative stress.

CIp10, an efficient and convenient integrating vector for Candida albicans.

Cloning, analysis and one-step disruption of the ARG5,6 gene of Candida albicans.

Contribution of the glycolytic flux and hypoxia adaptation to efficient biofilm formation by Candida albicans.

COS1, a two-component histidine kinase that is involved in hyphal development in the opportunistic pathogen Candida albicans.

Defective hyphal development and avirulence caused by a deletion of the SSK1 response regulator gene in Candida albicans.

Ectopic expression of URA3 can influence the virulence phenotypes and proteome of Candida albicans but can be overcome by targeted reintegration of URA3 at the RPS10 locus.

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Functional studies of the Ssk1p response regulator protein of Candida albicans as determined by phenotypic analysis of receiver domain point mutants.

Fungal histidine kinases. Sci STKE

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Glutamate at the site of phosphorylation of nitrogen-regulatory protein NTRC mimics aspartyl-phosphate and activates the protein.

Identification of a putative histidine kinase two-component phosphorelay gene (CaHK1)i nCandida albicans.

Identification of a putative response regulator two-component phosphorelay gene (CaSSK1) from Candida albicans.

Identification of YPD1, a gene of Candida albicans which encodes a two-component phosphohistidine intermediate protein.

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Msb2 signaling mucin controls activation of Cek1 mitogen-activated protein kinase in Candida albicans.

Multiple pathways differentially regulate global oxidative stress responses in fission yeast.

Multistep phosphorelay proteins transmit oxidative stress signals to the fission yeast stress-activated protein kinase.

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Saccharomyces cerevisiae histidine phosphotransferase Ypd1p shuttles between the nucleus and cytoplasm for SLN1-dependent phosphorylation of

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Signaling through adenylyl cyclase is essential for hyphal growth and virulence in the pathogenic fungus Candida albicans.

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Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans.

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The eukaryotic twocomponent histidine kinase Sln1p regulates OCH1 via the transcription factor,

The Sho1 adaptor protein links oxidative stress to morphogenesis and cell wall biosynthesis in the fungal pathogen Candida albicans.

The Skn7 response regulator controls gene expression in the oxidative stress response of the budding yeast Saccharomyces cerevisiae.

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Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 ‘‘two-component’’ osmosensor.

Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure.

Yeast Skn7p functions in a eukaryotic two-component regulatory pathway.

Identification of a novel response regulator, Crr1, that is required for hydrogen peroxide resistance in Candida albicans

Candida albicans colonises numerous niches within humans and thus its success as a pathogen is dependent on its ability to adapt to diverse growth environments within the host. Two component signal transduction is a common mechanism by which bacteria respond to environmental stimuli and, although less common, two component-related pathways have also been characterised in fungi. Here we report the identification and characterisation of a novel two component response regulator protein in C. albicans which we have named CRR1 (Candida Response Regulator 1). Crr1 contains a receiver domain characteristic of response regulator proteins, including the conserved aspartate that receives phosphate from an upstream histidine kinase. Significantly, orthologues of CRR1 are present only in fungi belonging to the Candida CTG clade. Deletion of the C. albicans CRR1 gene, or mutation of the predicted phospho-aspartate, causes increased sensitivity of cells to the oxidising agent hydrogen peroxide. Crr1 is present in both the cytoplasm and nucleus, and this localisation is unaffected by oxidative stress or mutation of the predicted phospho-aspartate. Furthermore, unlike the Ssk1 response regulator, Crr1 is not required for the hydrogen peroxide-induced activation of the Hog1 stress-activated protein kinase pathway, or for the virulence of C. albicans in a mouse model of systemic disease. Taken together, our data suggest that Crr1, a novel response regulator restricted to the Candida CTG clade, regulates the response of C. albicans cells to hydrogen peroxide in a Hog1-independent manner that requires the function of the conserved phospho-aspartate

Catherine R Bruce

Deborah A Smith

David Rodgers

Alessandra da Silva Dantas

Donna M MacCallum

Brian A Morgan

Janet Quinn

Directory of Open Access Journals

Identification of a novel response regulator, Crr1, that is required for hydrogen peroxide resistance in Candida albicans.

Catherine R. Bruce

Deborah A. Smith

Donna M. MacCallum

Brian A. Morgan

Crossref

Identification of a Novel Response Regulator, Crr1, That Is Required for Hydrogen Peroxide Resistance in Candida albicans

https://figshare.com/articles/Identification_of_a_Novel_Response_Regulator_Crr1_That_Is_Required_for_Hydrogen_Peroxide_Resistance_in_Candida_albicans_/130853

Identification of a novel response regulator, Crr1, that is required for hydrogen peroxide resistance in Candida albicans

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