Transgenic Drosophila are used to identify the functions of a small set of secretory cells that are typically associated with the sperm-storage organs of female insects

A Bergmann

A Kapelnikov

A Prokupek

A Thomas

A. C Spradling

A. D Stewart

A. H Brand

A. K Allen

A. M Collins

A. M Prokupek

B Baer

B. A Hassan

C Gillott

D. G Gilbert

D. M Neubaum

D. W Rogers

E Mancini

E. J Villavaso

E. S Kelleher

F Catteruccia

F. W Avila

G Dietzl

G. M Rubin

J Peng

K Iida

K. W Holzworth

L. A McGraw

L. K Sirot

M Filosi

M. C Bloch Qazi

M. F Wolfner

M. K Lawniczak

M. K Manier

M. K. N Lawniczak

M. N Arbeitman

Mariana Federica Wolfner

Mark L. Siegal

N Yapici

N. M Fernandez

P. D Mack

P. M Salvaterra

R. C Anderson

S Barolo

S Pitnick

Sandra L. Schnakenberg

T Chapman

T. A Markow

T. M Panhuis

W. J Swanson

W. R Rice

Wilfredo R. Matias

Y Heifetz

Y. S Lee

PLoS Biology

English

PubMed

Male Drosophila flies secrete seminal-fluid proteins that mediate proper sperm storage and fertilization, and that induce changes in female behavior. Females also produce reproductive-tract secretions, yet their contributions to postmating physiology are poorly understood. Large secretory cells line the female's spermathecae, a pair of sperm-storage organs. We identified the regulatory regions controlling transcription of two genes exclusively expressed in these spermathecal secretory cells (SSC): Spermathecal endopeptidase 1 (Send1), which is expressed in both unmated and mated females, and Spermathecal endopeptidase 2 (Send2), which is induced by mating. We used these regulatory sequences to perform precise genetic ablations of the SSC at distinct time points relative to mating. We show that the SSC are required for recruiting sperm to the spermathecae, but not for retaining sperm there. The SSC also act at a distance in the reproductive tract, in that their ablation: (1) reduces sperm motility in the female's other sperm-storage organ, the seminal receptacle; and (2) causes ovoviviparity--the retention and internal development of fertilized eggs. These results establish the reproductive functions of the SSC, shed light on the evolution of live birth, and open new avenues for studying and manipulating female fertility in insects

Sandra L Schnakenberg

Wilfredo R Matias

Mark L Siegal

Directory of Open Access Journals

Sperm-storage defects and live birth in Drosophila females lacking spermathecal secretory cells.

Crossref

Sperm-Storage Defects and Live Birth in Drosophila Females Lacking Spermathecal Secretory Cells

Schnakenberg Sandra L.

Matias Wilfredo R.

Siegal Mark L.

Public Library of Science (PLOS)

 Females Lacking Spermathecal Secretory Cells

A genome-wide analysis of courting and mating responses in Drosophila melanogaster females.

A genomewide transgenic RNAi library for conditional gene inactivation in Drosophila.

A genomic analysis of Drosophila somatic sexual differentiation and its regulation.

A receptor that mediates the post-mating switch in Drosophila reproductive behaviour.

A study of the factors affecting fertility of lozenge females of Drosophila melanogaster.

An assessment of sperm survival in Drosophila melanogaster.

An evolutionary expressed sequence tag analysis of Drosophila spermatheca genes.

Aquadro CF

atonal regulates neurite arborization but does not act as a proneural gene in the Drosophila brain.

Battle and ballet: molecular interactions between the sexes in Drosophila.

Beyond the mouse model: using Drosophila as a model for sperm interaction with the female reproductive tract.

Control of oocyte production, development, and release in Drosophila melanogaster.

Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products.

Diversity-enhancing selection acts on a female reproductive protease family in four subspecies of Drosophila mojavensis.

Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP.

Duplication, selection and gene conversion in a Drosophila mojavensis female reproductive protein family.

Egg size, embryonic development time and ovoviviparity in Drosophila species.

Ejaculate esterase 6 and initial sperm use by female Drosophila melanogaster.

Evolution of multiple kinds of female sperm-storage organs in Drosophila.

Evolutionary conflicts of interest between males and females.

Fine structure of spermatheca of Drosophila melanogaster Meig.

Gene duplication and adaptive evolution of digestive proteases in Drosophila arizonae female reproductive tracts.

Gene expression during the life cycle of Drosophila melanogaster.

Genes regulated by mating, sperm, or seminal proteins in mated female Drosophila melanogaster.

Genetic transformation of Drosophila with transposable element vectors.

Glucose dehydrogenase is required for normal sperm storage and utilization in female Drosophila melanogaster.

Gradual release of sperm bound sex-peptide controls female postmating behavior in Drosophila.

Insect seminal fluid proteins: identification and function.

Insights into female sperm storage from the spermathecal fluid proteome of the honeybee Apis mellifera.

Making a better RNAi vector for Drosophila: use of intron spacers.

Male accessory gland secretions: modulators of female reproductive physiology and behavior.

Male accessory gland substances modify the host-seeking behavior of gravid Aedes aegypti mosquitoes.

Mating induces an immune response and developmental switch in the Drosophila oviduct.

Mating-responsive genes in reproductive tissues of female Drosophila melanogaster.

Molecular and cellular components of the mating machinery in Anopheles gambiae females.

Molecular evolution and population genetic analysis of candidate female reproductive genes in Drosophila.

Molecular evolution of a gene cluster of serine proteases expressed in the Anopheles gambiae female reproductive tract.

Molecular population genetics of femaleexpressed mating-induced serine proteases in Drosophila melanogaster.

Nervous control of egg progression into the common oviduct and genital chamber of the stick-insect Carausius morosus.

New Drosophila transgenic reporters: insulated P-element vectors expressing fast-maturing RFP.

Proteomic analyses of male contributions to honey bee sperm storage and mating.

Regulation of cell number by MAPK-dependent control of apoptosis: a mechanism for trophic survival signaling.

Reproductive biology of invertebrates.

Resolving mechanisms of competitive fertilization success in Drosophila melanogaster.

Sex peptide is required for the efficient release of stored sperm in mated Drosophila females.

Sexually antagonistic male adaptation triggered by experimental arrest of female evolution.

Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila.

The ro ˆle of the spermathecal gland of the boll weevil, Anthonomus grandis.

The seminal fluid proteome of the honeybee Apis mellifera.

The Sf1-related nuclear hormone receptor Hr39 regulates Drosophila female reproductive tract development and function.

Tissue remodeling: a mating-induced differentiation program for the Drosophila oviduct.

Transcriptional profiling of the sperm storage organs of Drosophila melanogaster.

Transfer and fate of seminal fluid molecules in the beetle, Diaprepes abbreviatus: implications for the reproductive biology of a pest species.

Transgenic technologies to induce sterility.

Transposition of cloned P elements into Drosophila germ line chromosomes.

Wise, winsome, or weird? Mechanisms of sperm storage in female animals.

http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.288.5617

Sperm-Storage Defects and Live Birth in Drosophila Females Lacking Spermathecal Secretory Cells

Abstract

Similar works

Full text

Available Versions

Directory of Open Access Journals

Crossref

Public Library of Science (PLOS)