A Behrens

A Tomlinson

AH Rantamäki

BD Sullivan

Bowen Li

C Joffre

CH Song

D Harvey

G Shui

G Subbanagounder

Guanghou Shui

GW Ousler

IA Butovich

J Arciniega

J Chen

J Wang

JA Hamilton

JC Wojtowicz

JM Tiffany

JP McCulley

JT Barr

JT Saville

KB Green-Church

KK Nichols

L Tong

L Zhou

Louis Tong

M Dogru

M Uchino

MA Lemp

Markus R. Wenk

MR Wenk

MZ Ashraf

N Leitinger

N Nicolaides

Naj Sharif

OD Schein

RM Schiffman

S Landreville

S Liu

Shyam S. Chaurasia

Siew Sian Yong

Sin Man Lam

TH Wakamatsu

W Fei

WD Mathers

WE Shine

Y Jie

English

PubMed

Previous lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite the higher prevalence of dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to the alterations in lipid profiles in relation to DTS onset and progression is also lacking and warrants comprehensive experimental analysis. mild, moderate and severe) based on the ocular surface disease index (OSDI). A significantly lower level of TAG (p<0.05) was observed in patients under the moderate category compared to the mild category. Notably, a number of OAHFA species displayed consistently decreasing levels that correlate with increasing disease severity. An attempt was also made to investigate the changes in meibum lipid profiles of DTS patients compared to normal individuals classified based on OSDI score. Several unsaturated TAG and PC species were found at significantly higher levels (p<0.05) in patients than controls.The current study presents, for the first time, a comprehensive lipidome of meibum from individuals of an Asian ethnicity, which can potentially offer new insights into the higher prevalence of DTS observed amongst Asian populations. This study also represents an attempt towards identification of lipid species in meibum which could serve as marker for DTS

Lam Sin Man

Tong Louis

Yong Siew Sian

Li Bowen

Chaurasia Shyam S.

Shui Guanghou

Wenk Markus R.

Public Library of Science (PLOS)

Meibum Lipid Composition in Asians with Dry Eye Disease

Crossref

PLoS ONE

Previous lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite the higher prevalence of dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to the alterations in lipid profiles in relation to DTS onset and progression is also lacking and warrants comprehensive experimental analysis.We examined the meibum lipidome of 27 DTS patients and 10 control subjects for a total of 256 lipid species from 12 major lipid classes, including cholesteryl ester (CE), wax ester (WE), triacylglyceride (TAG), (O-acyl)-ω-hydroxy fatty acid (OAHFA), glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG) and sphingolipids (sphingomyelin, SM; ceramide, Cer; glucosylceramide, GluCer; dihexosylceramide, DihexCer). Neutral lipids were analysed using high-performance liquid-chromatography coupled with mass spectrometry (HPLC/MS) and tandem mass spectrometry (MS/MS) was used for the qualitative and quantitative analysis of polar lipid species. DTS patients were classified into three severity groups (i.e. mild, moderate and severe) based on the ocular surface disease index (OSDI). A significantly lower level of TAG (p<0.05) was observed in patients under the moderate category compared to the mild category. Notably, a number of OAHFA species displayed consistently decreasing levels that correlate with increasing disease severity. An attempt was also made to investigate the changes in meibum lipid profiles of DTS patients compared to normal individuals classified based on OSDI score. Several unsaturated TAG and PC species were found at significantly higher levels (p<0.05) in patients than controls.The current study presents, for the first time, a comprehensive lipidome of meibum from individuals of an Asian ethnicity, which can potentially offer new insights into the higher prevalence of DTS observed amongst Asian populations. This study also represents an attempt towards identification of lipid species in meibum which could serve as marker for DTS

Shyam S Chaurasia

Markus R Wenk

Directory of Open Access Journals

Meibum lipid composition in Asians with dry eye disease.

A compositional based model for the tear film lipid layer. Trans Am Ophthalmol Soc 95: 79–88; discussion 88.

Advances in understanding and managing dry eye disease.

An objective approach to dry eye disease severity.

Association of tear proteins with Meibomian gland disease and dry eye symptoms.

Bioactive products of phospholipid oxidation: isolation, identification, measurement and activities.

Changes in gene expression in human meibomian gland dysfunction.

Changing trends in the definition and diagnosis of dry eyes.

Cholesteryl esters as a depot for very long chain fatty acids in human meibum.

Corneal scarring in the Collaborative Longitudinal Evaluation of Keratoconus (CLEK) Study: baseline prevalence and repeatability of detection.

Differences in meibomian fatty acid composition in patients with meibomian gland dysfunction and aqueous-deficient dry eye.

Dysfunctional tear syndrome: a Delphi approach to treatment recommendations.

Effects of ceramides on meibomian lipid layers in vitro.

Enhanced secretory group II PLA2 activity in the tears of chronic blepharitis patients.

Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast.

Historical brief on composition of human meibum lipids.

Human tear film and meibum. Very long chain wax esters and (O-acyl)-omega-hydroxy fatty acids of meibum.

Human tear fluid lipidome: from composition to function.

Identification by combined gas chromatography-mass spectrometry of constituent long-chain fatty acids and alcohols from the meibomian glands of the rat and a comparison with human meibomian lipids.

Identification of fatty acids and fatty acid amides in human meibomian gland secretions.

Identification of phospholipids in human meibum by nano-electrospray ionisation tandem mass spectrometry.

Identification of tear fluid biomarkers in dry eye syndrome using iTRAQ quantitative proteomics.

Individual variations in human meibomian lipid composition.

Influence of aging on the polar and neutral lipid profiles in human meibomian gland secretions.

Interactions of triglycerides with phospholipids: incorporation into the bilayer structure and formation of emulsions.

Lipidomics of human Meibomian gland secretions: Chemistry, biophysics, and physiological role of Meibomian lipids.

Lipids of human meibum: massspectrometric analysis and structural elucidation.

Liquid chromatography-mass spectrometric analysis of lipids present in human meibomian gland secretions.

Meibomian gland lipids, evaporation, and tear film stability.

Meibomian gland studies: comparison of steer and human lipids.

Methodologies for the study of ocular surface disease.

On the lipid composition of human meibum and tears: comparative analysis of nonpolar lipids.

Oxidized phospholipids as modulators of inflammation in atherosclerosis.

Oxidized phospholipids: biomarker for cardiovascular diseases.

Phospholipases A2 in ocular homeostasis and diseases.

Polar lipids in human meibomian gland secretions.

Prevalence of dry eye among the elderly.

Reliability and validity of the Ocular Surface Disease Index.

Shotgun lipidomic analysis of human meibomian gland secretions with electrospray ionization tandem mass spectrometry.

Tearful relations: oxidative stress, inflammation and eye diseases.

The definition and classification of dry eye disease: report of the Definition and

The emerging field of lipidomics.

The epidemiology of dry eye disease: report of the Epidemiology Subcommittee of the International Dry Eye WorkShop

The features of dry eye disease in a Japanese elderly population.

The international workshop on meibomian gland dysfunction: report of the diagnosis subcommittee.

The international workshop on meibomian gland dysfunction: report of the subcommittee on tear film lipids and lipid-protein interactions in health and disease.

The lipid layer of tears: dependent on meibomian gland function.

The Meibomian puzzle: combining pieces together.

Toward one step analysis of cellular lipidomes using liquid chromatography coupled with mass spectrometry: application to Saccharomyces cerevisiae and Schizosaccharomyces pombe lipidomics.

file:///data/remote/core/dit/data/public_library_of_science/pdf/db4/ZnRwOi8vZnRwLm5jYmkubmxtLm5paC5nb3YvcHViL3BtYy9vYV9wYWNrYWdlLzJmL2U1L1BNQzMxOTcxOTYudGFyLmd6.pdf

Meibum Lipid Composition in Asians with Dry Eye Disease

Abstract

Similar works

Full text

Available Versions

Public Library of Science (PLOS)

Crossref

Directory of Open Access Journals

Public Library of Science (PLOS)