AMORESANO, ANGELA

CHIAIESE, Pasquale

FILIPPONE, EDGARDO

Archivio della ricerca - Università degli studi di Napoli Federico II

Proceedings of the Joint Congress SIBV-SIGAMilano, Italy – 8/11 September, 2015ISBN 978-88-904570-5-0Poster Communication Abstract – B.34A COMPARATIVE IONOMIC ANALYSIS OF SOLANUM MELONGENAPLANTS TREATED WITH ARSENIC OR CHROMIUMRUIU F.*, DI SALVATORE M.*, ROMANO G.*, SORRENTINO G.*, AMORESANO A.**,SALLUZZO A.***, FILIPPONE E.*, CHIAIESE P.**) Department of Agricultural Sciences, University of Naples “Federico II”, Via Università 100,80055 Portici (Italy)**) Department of Chemistry, University of Naples “Federico II”***) ENEA, UTTP-CHIA, Portici Research Centerionomic, eggplant, arsenic, chromium, qPCRThe cell plants possess a wide array of ions transporter proteins allowing to regulate theuptake, the distribution and the accumulation of mineral in the plants. However, these processeslack of high selectivity and therefore plants can also absorb non-essential elements such as heavymetals. The accumulation of some elements such as As, Cd, Cr and Pb in edible part of crops ishazardous for human health. Among these elements we have focused our attention on arsenic (As)and chromium (Cr). The content of these two elements in agriculture soils may be due toanthropogenic activities or the paedogenesis of agricultural soil such as them from volcanic area.Arsenic enters, mainly, in the plant by different families of transporters as: nodulin 26-like intrinsicprotein (NIP), High-affinity phosphate (PHT), ATP-binding cassette (ABC). On the other hands,plants do not possess specific mechanisms for the absorption of chromium but it has been reportedthat it uses the “ionic mimicry”. Among the ions transporter gene family, the SULTR proteins arethe main candidates to recognise Cr. In addition, the Metal Tolerance Protein (MTP) and themetallothioneins (MT), are proteins that are regulate the content of several elements. Eggplant is aplant widely cultivated in many parts of the world and in Italy the largest production in the southernareas. In this study, we report the change in ionomic and expression of some ion transporters inroots stem, and leaf of the following cultivars: Bellezza Nera (BN); Purple Queen (PQ); Cima Viola(CV); Violetta di Firenze (VF); Violetta Lunga 2 (VL); Rotonda Bianca Sfumata di Rosa (RB). Theeffects of As and Cr in form of sulphate was assayed in hydroponic culture at the concentration of5, µM. The treatment was performed on 30 days-old plants. The analysis obtained by ICP-MSshowed that eggplants roots are the main organs that accumulates As and Cr. A different index ofbioaccumulation was determined in the root of the 6 cultivars assayed. Based on such index wehave determined the minus and plus cultivars for each elements assayed. In addition, thetranslocation factors shown that As and Cr were not easily translocated to the aboveground organs.The ionome of roots, stems and leaves of eggplant varied in presence of the two elements addedinto the hydroponic solution. By a bioinformatic approach it was possible to identify eight genesrelated to As and Cr uptake transport and accumulation. Those genes modified their expressionaccording to treatment and /or genotype in an organ specific manner.

A COMPARATIVE IONOMIC ANALYSIS OF SOLANUM MELONGENA PLANTS TREATED WITH ARSENIC OR CHROMIUM

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Archivio della ricerca - Università degli studi di Napoli Federico II