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Arie B. Brinkman

Cornelia G. Spruijt

Hendrik G. Stunnenberg

Michiel Vermeulen

Pascal W. T. C. Jansen

Pierre-Antoine Defossez

Stefanie J. J. Bartels

PLoS ONE

English

PubMed

Crossref

A SILAC-Based Screen for Methyl-CpG Binding Proteins Identifies RBP-J as a DNA Methylation and Sequence-Specific Binding Protein

Bartels, S.J.J.

Spruijt, C.G.

Brinkman, A.B.

Jansen, P.

Vermeulen, M.

Stunnenberg, H.G.

NARCIS 

DNA methylation is an epigenetic modification that plays a crucial role in a variety of biological processes. Methylated DNA is specifically bound by Methyl-CpG Binding Proteins (MBPs). Three different types of MBPs have been identified so far: the Methyl-CpG Binding Domain (MBD) family proteins, three BTB/POZ-Zn-finger proteins, and UHRF1. Most of the known MBPs have been identified via homology with the MBD and Zn-finger domains as present in MeCP2 and Kaiso, respectively. It is conceivable that other proteins are capable of recognizing methylated DNA.For the purpose of identifying novel ‘readers’ we set up a methyl-CpG pull-down assay combined with stable-isotope labeling by amino acids in cell culture (SILAC). In a methyl-CpG pull-down with U937 nuclear extracts, we recovered several known MBPs and almost all subunits of the MBD2/NuRD complex as methylation specific binders, providing proof-of-principle. Interestingly, RBP-J, the transcription factor downstream of Notch receptors, also bound the DNA in a methylation dependent manner. Follow-up pull-downs and electrophoretic mobility shift assays (EMSAs) showed that RBP-J binds methylated DNA in the context of a mutated RBP-J consensus motif.The here described SILAC/methyl-CpG pull-down constitutes a new approach to identify potential novel DNAme readers and will advance unraveling of the complete methyl-DNA interactome

Bartels Stefanie J. J.

Spruijt Cornelia G.

Brinkman Arie B.

Jansen Pascal W. T. C.

Vermeulen Michiel

Stunnenberg Hendrik G.

Public Library of Science (PLOS)

Radboud Repository

Abstract Background: DNA methylation is an epigenetic modification that plays a crucial role in a variety of biological processes. Methylated DNA is specifically bound by Methyl-CpG Binding Proteins (MBPs). Three different types of MBPs have been identified so far: the Methyl-CpG Binding Domain (MBD) family proteins, three BTB/POZ-Zn-finger proteins, and UHRF1. Most of the known MBPs have been identified via homology with the MBD and Zn-finger domains as present in MeCP2 and Kaiso, respectively. It is conceivable that other proteins are capable of recognizing methylated DNA

Stefanie J J Bartels

Cornelia G Spruijt

Arie B Brinkman

Pascal W T C Jansen

Hendrik G Stunnenberg

CiteSeerX

A SILAC-based screen for methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein

Bird A

Many paths to one goal? The proteins that recognize methylated DNA in eukaryotes.

Methyl-CpG binding proteins: specialized transcriptional repressors or structural components of chromatin?

Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells.

A SILAC-Based Screen for Methyl-CpG Binding Proteins Identifies RBP-J as a DNA Methylation and Sequence-Specific Binding Protein

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Public Library of Science (PLOS)

Radboud Repository

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