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Individual Actin Filaments in a Microfluidic Flow Reveal the Mechanism of ATP Hydrolysis and Give Insight Into the Properties of Profilin
Authors
Antoine Jégou
B Bugyi
+43 more
C Combeau
C Le Clainche
C Valentin-Ranc
D Pantaloni
D Vavylonis
Dominique Didry
E. B Stukalin
E. D Korn
E. G Yarmola
E. G Yarmola
Guillaume Romet-Lemonne
H Isambert
H. J Kinosian
H. J Kinosian
H. Y Kueh
I Fujiwara
I Fujiwara
I Gutsche-Perelroizen
J. A Spudich
J. R Kuhn
József Orbán
K Murakami
L Blanchoin
L Blanchoin
L. R Brewer
M Bindschadler
M. B Smith
M. F Carlier
M. F Carlier
M. F Carlier
M. R Bubb
Marie-France Carlier
P Ranjith
P Tabeling
R Gieselmann
R Melki
Reinhard Lipowsky
S Romero
T Ohm
T. D Pollard
Thomas Niedermayer
Thomas Pollard
X Li
Publication date
1 January 2011
Publisher
Public Library of Science
Doi
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on
PubMed
Abstract
A novel microfluidic approach allows the analysis of the dynamics of individual actin filaments, revealing both their local ADP/ADP-Pi-actin composition and that Pi release is a random mechanism
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