Comparison of extenders for liquid storage of Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa

Abstract

This study was designed to evaluate a range of avian semen extenders for liquid storage of Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa at 5 °C. Semen was collected from 8 mature trained cocks and processed in the Beltsville Poultry, Turkey, Lake, EK, Tselutin Poultry and Chicken semen extenders for storage at 5 °C. Semen quality parameters viz, motility (%), plasma membrane integrity (%), livability (%) and acrosomal integrity (%) were assessed at 0, 3, 6, 24 and 48 hours of storage. A time dependent decrease was observed in motility, plasma membrane and acrosomal integrity in all experimental extenders during the storage period. However, the Turkey semen extender was found significantly (P<0.05) superior for protecting all aforementioned semen quality parameters compared to the Beltsville Poultry, Lake, EK, Tselutin Poultry and Chicken semen extenders. It is concluded that the Turkey semen extender can be used efficiently for the liquid storage of Indian Red Jungle Fowl spermatozoa at 5 °C