open8noopenDotto, G.; Pasotto, D.; Poser, H.; Menandro, M.L.; Berlanda, M.; Falomo, M.E.; Mondin, A.; Martini, M.Dotto, Giorgia; Pasotto, Daniela; Poser, Helen; Menandro, MARIA LUISA; Berlanda, Michele; Falomo, MARIA ELENA; Mondin, Alessandra; Martini, Marc

Berlanda, Michele

Dotto, Giorgia

Falomo, Maria Elena

Martini, Marco

Menandro, Maria Luisa

Mondin, Alessandra

Pasotto, Daniela

Poser, Helen

International Journal of Infectious Diseases

English

Padua Research Archive

Evaluation of pet animals involved in assisted interventions (AAI) as potential carriers of bacteria resistant to antimicrobials: Preliminary data

DOTTO, GIORGIA

PASOTTO, DANIELA

POSER, HELEN

MENANDRO, MARIA LUISA

BERLANDA, MICHELE

FALOMO, MARIA ELENA

MONDIN, ALESSANDRA

MARTINI, MARCO

Archivio istituzionale della ricerca - Università di Padova

76 Abstracts / International Journal of Infectious Diseases 53S (2016) 4–163Results: From 2000-2015 a total of 57 cases of tularemia werereported in Armenia. Of these, 84.2% were male and 78.9% werefrom rural areas. Distribution of age groups were: ≤18–24.6%,19-30–54.4%, 31-44–14.01%, ≥45–7.0%. The most common symp-toms include: fatigue (n=52, 91%), fever (n=45, 78.9%), pharyngitis(n=40, 70.2%), headache (n=27, 47.4%), hepatomegaly (n=5, 8.8%),and splenomegaly (n=2, 3.5%). The average hospitalization staywas 35.7 days. The most frequent clinical forms were bubonic(n=28, 49.1%), oropharyngeal (n=21, 36.9%) and oculoglandular(n=8, 14%). In all patients enlargement of the lymph nodes wasreported, most frequently the cervical ones (n=52, 91.2%). Ultra-sound imaging showed hepatosplenomegaly in three cases (5.2%).Antibiotics were given prior to admission for 26 (45.6%) patientsand during hospitalization for 54 (94.8%). Twenty-three (40.4%)received monotherapy while 31 (54.4%) received combined ther-apy. Diagnosis was based on agglutination reaction in most cases(n=50, 87.7%). For seven patients, diagnosis was made based ononly clinical-epidemiological history without laboratory conﬁrma-tion (n=7, 12.3%). Surgical treatment was used for 8.8% patients.Presence of rodents at household was mentioned by 14 (24.6%),while four (7%) noted a vector bite and three (5.3%) had contactwith rodents. Over half (56.2%) had centralized water.Conclusion: Most of the patients were male and from ruralareas. People from 19-30 years of age were more likely to contracttularemia. Approximatelyhalf of thepatients had thebubonic form,mainly cervical. Other potential risk factors that might explain along hospitalization (e.g. suppressed immune system, treatmentfailure etc.), should be a topic for future investigations.http://dx.doi.org/10.1016/j.ijid.2016.11.19119.141Evaluation of pet animals involved in assistedinterventions (AAI) as potential carriers ofbacteria resistant to antimicrobials:Preliminary dataG. Dotto ∗, D. Pasotto, H. Poser, M.L. Menandro, M.Berlanda, M.E. Falomo, A. Mondin, M. MartiniUniversity of Padua, Department of AnimalMedicine, Production and Health, Legnaro - Agripolis(PD)/ITPurpose: The attention paid to pets involved in Animal-AssistedInterventions (AAI) for their therapeutic beneﬁts to humans isincreasing worldwide. Their close contact with vulnerable cate-gories prompts the consideration of the transmission of zoonoticpathogens between animals and patients. This study is aimed toverify the health conditions of pets used for AAI and to evalu-ate the potential spread of pathogens to humans, especially thosecausing severe hospital and community acquired-infections (HAI)such as extended-spectrum cephalosporin and carbapenem resis-tant Enterobacteriaceae, other Gram-negative multidrug-resistantbacteria (such as Pseudomonas aeruginosa, Acinetobacter bau-mannii, erythromycin and ciproﬂoxacin resistant Campylobacterspp., e.g.), methicillin-resistant Staphylococcus spp., vancomycin-resistant Enterococcus spp. and toxigenic Clostridium difﬁcile.Methods & Materials: Clinical examination of 65 animals (42dogs, 22 donkeys and 1 horse, owned by 19 different thera-pists/handlers) involved in AAI was assessed. Faecal, perineal andoral/nasal sampleswere processed following classicalmicrobiolog-ical procedures. The phenotypic antimicrobial-resistance proﬁleswere determined by selective/chromogenic media supplementedwith speciﬁc drugs or by the disk diffusion method according tothe CLSI guidelines.Results: The 65 animals tested were all in good health andnone carried multidrug-resistant Acinetobacter baumannii, Pseu-domonas aeruginosa and Klebsiella spp. nor Salmonella spp. Of the42 dog sampled, 3 (7%) were positive to thermophilic Campy-lobacter spp., 1 (2%) to extended-spectrum cephalosporin-resistantE. coli, 6 (14%) to vancomycin-resistant Enterococcus spp., 3 (7%)to methicillin-resistant Staphylococcus spp., 1 (2%) to multidrug-resistant Stenotrophomonas maltophilia and 1 (2%) to C. difﬁcile,respectively. Lastly, 1 (5%) donkey was positive to methicillin-resistant Staphylococcus spp..Conclusion: These preliminary data highlight the good healthconditions of the animals tested. Only few pets carried poten-tially zoonotic antimicrobial-resistant human pathogens. Thesedata conﬁrm that HAI are mainly associated with the spreadof pathogens through person-to-person transmission and medi-cal devices contaminations. However, our ﬁndings suggest thatthe presence of potential pathogens with resistant traits is notirrelevant and should be evaluated especially in animals in close-contact with immunocompromised/diseased persons. The lack ofguidelines and of a systematic surveillance on pets involved inAAIs as possible asymptomatic carriers of antimicrobial-resistantpathogens, poses the need to investigate their role in the transmis-sion of these zoonoses to humans.http://dx.doi.org/10.1016/j.ijid.2016.11.19219.142Seropositivity of delta hepatitis in patients withpositive hepatitis B surface antigen, between2011 and 2016M. Ugura, S. Ünlüb, C. Eryıldıza,∗, N. Sakrua, A.Tezel ca Trakya University, Faculty of Medicine, Departmentof Medical Microbiology, Edirne/TRb Edirne State Hospital, Clinic of MedicalMicrobiology, Edirne/TRc Trakya University, Faculty of Medicine, Departmentof Gastroenterology, Edirne/TRPurpose: Hepatitis delta virus is defective RNA virus thatrequireshepatitis Bvirus to cause infection. Co-infectionand super-infection are two forms of HDV according to the association withHBV. The prevalence of HDV infection is less thanHBV infection butthe clinical feature caused by HDV infection is more severe.Methods & Materials: This study included a total of 1131HBsAg positive serum samples which have sent to Microbiol-ogy Department of Trakya University Health Center for MedicalResearch and Practice in Edirne, between 2011 and 2016. Deltaantibodies in HBsAg positive 1131 serum samples were studied bymethods micro-ELISA (Diagnostic Bioprobes, Italy) HBsAg, HBeAg,anti-HBe were studied by chemiluminescenceassay (Roche Diag-nostics, USA). The data of patients were evaluated, retrospectively.Results: Twenty eight of 1131 HbsAg positive serumwere iden-tiﬁed as positive in terms of delta antibodies. The ratio of HDV inHBsAg positive patients was found to be 2.47%. Twenty seven andoneout of twenty eight patientswere determined as superinfectionand co-infection, respectively.Conclusion: Our country is located in an intermediate endemicarea for HDV. Although, seropositivity of HDV in this region hasfound a lower than the other regions of Turkey but also especiallychronic HBV patients should be screened for HDV.http://dx.doi.org/10.1016/j.ijid.2016.11.193

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Evaluation of pet animals involved in assisted interventions (AAI) as potential carriers of bacteria resistant to antimicrobials: Preliminary data

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