DOTTO, GIORGIA

MARTINI, MARCO

MENANDRO, MARIA LUISA

MONDIN, ALESSANDRA

PASOTTO, DANIELA

International Journal of Infectious Diseases

English

D. Pasotto

G. Dotto

M.L. Menandro

A. Mondin

M. Martini

Crossref

Prevalence and antimicrobial-resistance characterization of vancomycin resistant enterococci (VRE) strains in healthy household dogs in Italy

Archivio istituzionale della ricerca - Università di Padova

Original Citation:Prevalence and antimicrobial-resistance characterization of vancomycin resistant enterococci (VRE)strains in healthy household dogs in Italyelsevier LtdPublisher:Published version:DOI:Terms of use:Open Access(Article begins on next page)This article is made available under terms and conditions applicable to Open Access Guidelines, as described athttp://www.unipd.it/download/file/fid/55401 (Italian only)Availability:This version is available at: 11577/3217655 since: 2020-03-11T14:37:41Zhttp://dx.doi.org/10.1016/j.ijid.2016.11.129Università degli Studi di PadovaPadua Research Archive - Institutional Repository50 Abstracts / International Journal of Infectious Diseases 53S (2016) 4–163also incompanionanimals tohaveacompletepictureof thedynam-ics of this phenomenon.http://dx.doi.org/10.1016/j.ijid.2016.11.12819.046Prevalence and antimicrobial-resistancecharacterization of vancomycin resistantenterococci (VRE) strains in healthy householddogs in ItalyD. Pasotto ∗, G. Dotto, M.L. Menandro, A. Mondin,M. MartiniUniversity of Padua, Department of AnimalMedicine, Production and Health, Legnaro - Agripolis(PD)/ITPurpose: Enterococci are one of the leading causes of noso-comial infections for humans and the emergence of vancomycin-resistant enterococci (VRE) and high-level aminoglycoside-resistant enterococci (HLRA) caused public health concern becausethese drugs are often one the few therapeutic alternatives for treat-ment of multidrug-resistant (MDR) enterococcal infections. Thewidespread effect of MDR enterococci is partially driven by theselective pressure produced by the use and overuse of drugs inboth human and veterinary medical practice. In recent decades,the analyses of their virulence and antimicrobial-resistance traitsin hospital-acquired infections have been extensively reported inhumans. However, less attention has been given to the role of petsas reservoir of MDR enterococci, despite their relationship withhumans. The aim of this study was to evaluate the prevalence andthe antimicrobial-resistance traits of VRE from household dogs.Methods & Materials: Faecal samples of 237 healthy house-hold dogswere tested to detect Enterococcus spp. using the classicalbacteriological procedure. The species identiﬁcationwas conﬁrmedby PCR assays as previously described. Antimicrobial-resistance tovancomycin was assessed by plating single colonies on BrillianceVRE Agar (Oxoid). The antimicrobial susceptibility to high-levelaminoglycosides and to other compounds (together with the con-ﬁrmation of vancomycin-resistance), was evaluated by the diskdiffusion method based on recommendations of the CLSI.Results: Enterococci were detected in 72% (n=170) 170 ofdogs and, in accordance with other studies, the most prevalentEnterococcus species were Enterococcus faecalis (n=118; 69.4%) andEnterococcus faecium (n=50; 29.4%). The 49% of the isolates (n=84)were VRE and 2.4% (n=2) and 21.5% (n=18) of VRE strains showedHLR to gentamycin and streptomycin respectively. Interestingly,most of these isolates were also classiﬁed as multidrug-resistant.Conclusion: These results demonstrate that dogs are commonlycolonized by antimicrobial-resistant enterococci and highlight therole of pets as reservoir of last-line drug resistances. Moreover, theclose contact with humans conﬁrms the possible transmission ofantimicrobial-resistant bacteria from dogs to humans. Therefore,the study stresses the need of surveilling this phenomenon also incompanion animals.http://dx.doi.org/10.1016/j.ijid.2016.11.12919.048Detection of carbapenemase production bymultidrug-resistant acinetobacter baumanniiisolates from selected wards in dr georgemukhari academic hospitalD.T. PhofaSefako Makgatho Health Sciences University,Microbiological Pathology, Pretoria, GAUTENG/ZAPurpose: Carbapenem-resistant Acinetobacter baumannii hasemerged as an important cause of nosocomial outbreaks world-wide. Detection of carbapenemase production by modiﬁed Hodgetest using Mueller Hinton agar has been reported to be less sen-sitive. The objectives of this study were two-fold: i. to comparethe performance of MacConkey agar (MA) and Mueller Hinton agar(MHA) for the detection of carbapenemases; ii. to determine thetype of carbapenemases that are produced by A. baumannii isolatesfrom Dr George Mukhari Academic Hospital (DGM).Methods & Materials: Twenty four stored carbapenem-resistant and 2 carbapenem-susceptible A. baumannii isolateswereidentiﬁed using Vitek 2 automated system (bioMerieux, France).The modiﬁed Hodge test (MHT) was done according to CLSI guide-lines on MHA (DMP, South Africa) and MA (DMP, South Africa)using Escherichia coli ATCC 25922 and meropenem disks. All theplateswere incubated at 370C overnight. Detection of carbapenem-resistance genes (OXA-23, OXA-40, OXA-58, SIM, VIM-1, VIM-2AND IMP-like) was done according to published molecular meth-ods.Results: Of the 24 carbapenem-resistant isolates which werescreened, 87.5% (21) and 62.5% (15) were found to be carbapene-mase positive byMHT onMA andMHA, respectively. The cloverleafpattern on MHA appears to be absent for some of the isolates butit is accentuated on MA. Both MHA and MA showed 50% false posi-tive result for the 2 susceptible isolates. Of the 24 resistant isolateswhich were screened for bla genes, 87.5% (21) and 25% (6) werefound to have OXA-23 and OXA-40, respectively.Conclusion: MA may be used in place of MHA for the screeningof carbapenem-resistant A. baumannii and thus will impact oninfection control practices. OXA-23 bla gene was the most preva-lent carbapenemase type at DGM Hospital. Though the numberswere small, the study conﬁrms that phenotypicmethods lack speci-ﬁcity and sensitivity, hence their results have to be conﬁrmed bymolecular methods.http://dx.doi.org/10.1016/j.ijid.2016.11.130

Pasotto, Daniela

Dotto, Giorgia

Menandro, Maria Luisa

Mondin, Alessandra

Martini, Marco

Padua Research Archive

https://www.research.unipd.it/bitstream/11577/3217655/1/1-s2.0-S1201971216313479-main.pdf

Prevalence and antimicrobial-resistance characterization of vancomycin resistant enterococci (VRE) strains in healthy household dogs in Italy

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