A Isles

B Enderby

B Ryall

DP Speert

DW Domaille

DW Nielson

EH Vock

ER Smith

F Ratjen

G Doring

H Yamamoto

I Kukavica-Ibrulj

J Ren

J Schubert

J-C Richard

Jeesun Lim

JL Kadurugamuwa

JM Brint

JRW Govan

Juyoung Yoon

KS Lee

L Li

LG Rahme

M Iwata

M Muller

MA Howland

N Soh

NT Liberati

P Drevinek

PA Castric

S Park

S Rajan

S-Y Chung

Sang-Tae Kim

SC Burdette

SC FitzSimmons

Seong-Won Nam

So Hyun Kim

Sungsu Park

Tanya Parish

TB Rasmussen

TF Boat

TN Demidova

V Teichgraber

W Wang

WF McCarthy

WL Cody

WM Leevy

X Chen

X Lou

Xiaoqiang Chen

Y-J Heo

You-Hee Cho

Z Ekmekci

Z Xu

PLoS ONE

English

PubMed

 complex (Bcc), commonly found in the lungs of cystic fibrosis (CF) patients, often produce cyanide (CN), which inhibits cellular respiration. CN in sputa is a potential biomarker for lung infection by CF pathogens. However, its actual concentration in the infected lungs is unknown. efficacy of antimicrobial treatments was tested in live mice by monitoring bacteriogenic CN in the lungs. imaging method was also found suitable for minimally invasive testing the efficacy of antibiotic compounds as well as for aiding the understanding of bacterial cyanogenesis in CF lungs

Nam Seong-Won

Chen Xiaoqiang

Lim Jeesun

Kim So Hyun

Kim Sang-Tae

Cho You-Hee

Yoon Juyoung

Park Sungsu

Public Library of Science (PLOS)

 Fluorescence Imaging of Bacteriogenic Cyanide in the Lungs of Live Mice Infected with Cystic Fibrosis Pathogens

BackgroundPseudomonas aeruginosa (PA) and Burkholderia cepacia complex (Bcc), commonly found in the lungs of cystic fibrosis (CF) patients, often produce cyanide (CN), which inhibits cellular respiration. CN in sputa is a potential biomarker for lung infection by CF pathogens. However, its actual concentration in the infected lungs is unknown.Methods and findingsThis work reports observation of CN in the lungs of mice infected with cyanogenic PA or Bcc strains using a CN fluorescent chemosensor (4',5'-fluorescein dicarboxaldehyde) with a whole animal imaging system. When the CN chemosensor was injected into the lungs of mice intratracheally infected with either PA or B. cepacia strains embedded in agar beads, CN was detected in the millimolar range (1.8 to 4 mM) in the infected lungs. CN concentration in PA-infected lungs rapidly increased within 24 hours but gradually decreased over the following days, while CN concentration in B. cepacia-infected lungs slowly increased, reaching a maximum at 5 days. CN concentrations correlated with the bacterial loads in the lungs. In vivo efficacy of antimicrobial treatments was tested in live mice by monitoring bacteriogenic CN in the lungs.ConclusionsThe in vivo imaging method was also found suitable for minimally invasive testing the efficacy of antibiotic compounds as well as for aiding the understanding of bacterial cyanogenesis in CF lungs

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In vivo fluorescence imaging of bacteriogenic cyanide in the lungs of live mice infected with cystic fibrosis pathogens.

Background: Pseudomonas aeruginosa (PA) and Burkholderia cepacia complex (Bcc), commonly found in the lungs of cystic fibrosis (CF) patients, often produce cyanide (CN), which inhibits cellular respiration. CN in sputa is a potential biomarker for lung infection by CF pathogens. However, its actual concentration in the infected lungs is unknown. Methods and Findings: This work reports observation of CN in the lungs of mice infected with cyanogenic PA or Bcc strains using a CN fluorescent chemosensor (49,59-fluorescein dicarboxaldehyde) with a whole animal imaging system. When the CN chemosensor was injected into the lungs of mice intratracheally infected with either PA or B. cepacia strains embedded in agar beads, CN was detected in the millimolar range (1.8 to 4 mM) in the infected lungs. CN concentration in PA-infected lungs rapidly increased within 24 hours but gradually decreased over the following days, while CN concentration in B. cepacia-infected lungs slowly increased, reaching a maximum at 5 days. CN concentrations correlated with the bacterial loads in the lungs. In vivo efficacy of antimicrobial treatments was tested in live mice by monitoring bacteriogenic CN in the lungs. Conclusions: The in vivo imaging method was also found suitable for minimally invasive testing the efficacy of antibioti

Seong-won Nam

Sang-tae Kim

You-hee Cho

Juyoung Yoon Sungsu

CiteSeerX

In Vivo Fluorescence Imaging of Bacteriogenic Cyanide in the Lungs of Live Mice Infected with Cystic Fibrosis

Crossref

In Vivo Fluorescence Imaging of Bacteriogenic Cyanide in the Lungs of Live Mice Infected with Cystic Fibrosis Pathogens

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In Vivo Fluorescence Imaging of Bacteriogenic Cyanide in the Lungs of Live Mice Infected with Cystic Fibrosis Pathogens

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