A de Greeff

AL Davidson

AS Leung

Baoli Zhu

C Chen

C Gianfaldoni

C Wang

Changjun Wang

CM Longshaw

Cuihua Liu

CW Wong

DL Hava

EF Nuwaysir

EL Abbot

F Berthelot-Herault

F Hasan

G Vanier

George F Gao

HE Smith

Hui Liao

I Derre

J Rollof

J Tang

Jiaqi Tang

Jing Li

JJ Staats

M Gohar

M Li

M Molla

M Mora

M Osaki

Ming Li

MT Holden

N Declerck

Na Lu

Nahuel Fittipaldi

R Rosini

Ruifen Zhang

Ruifu Yang

S Dubrac

S Falker

S Quessy

S Sriskandan

S Wilhelm

T Lefebure

TJ Albert

U Vecht

Y Feng

Y Ma

Yongqiang Jiang

Zuowei Wu

BMC Genomics

English

PubMed

Springer - Publisher Connector

Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays

Abstract
          
            Background
            Our previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS) is associated with specific Streptococcus suis serotype 2 (SS2) strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays.
          
          
            Results
            Our results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI), which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs) across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into Streptococcus pathogenicity and evolution.
          
          
            Conclusions
            Our deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains.
          </jats:sec

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Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays

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