Fujisawa, Jun-ichi

Takenouchi, Norihiro

Tanaka, Masakazu

Tei, Mami

Tezuka, Kenta

Ueno, Takaharu

Xun, Runze

English

PubMed

Fujisawa Jun-ichi

Takenouchi Norihiro

Tanaka Masakazu

Ueno Takaharu

Tei Mami

Xun Runze

Tezuka Kenta

Directory of Open Access Journals

Retrovirology

Inverse correlation between Tax and CD25 expressions in HTLV-1 infected CD4 T-cells in vivo

Kenta Tezuka

Runze Xun

Mami Tei

Takaharu Ueno

Masakazu Tanaka

Norihiro Takenouchi

Jun-ichi Fujisawa

Springer - Publisher Connector

MEETING ABSTRACT Open Access
Inverse correlation between Tax and CD25
expressions in HTLV-1 infected CD4 T-cells in vivo
Kenta Tezuka, Runze Xun, Mami Tei, Takaharu Ueno, Masakazu Tanaka, Norihiro Takenouchi, Jun-ichi Fujisawa*
From 15th International Conference on Human Retroviruses: HTLV and Related Viruses
Leuven and Gembloux, Belgium. 5-8 June 2011
Tax has pleiotropic actions that induce proliferation and
inhibit apoptosis of T-cells and thus is considered to
play a critical role in leukemogenesis. However, Tax
expression is frequently lost in fresh adult T-cell leuke-
mia (ATL) cells by genetic changes or epigenetic modifi-
cations of proviral genome. To clarify the significance of
tax gene in leukemogenesis, we analyzed the expression
of tax gene in the HTLV-1 infected human CD4+ T-
lymphocytes in humanized mouse system.
NOG-SCID mouse were transplanted with human
hematopoietic stem cells and infected with HTLV-1 in
vivo by peritoneal injection of MT-2 cells after 4
months of transplantation. HTLV-1 infection induced
the rapid proliferation of CD4+ T-cells, irrespective of
the expression of CD25, and resulted in the severe sple-
nomegaly with CD25+ CD4+ T-cells. Tax gene expres-
sion was low in the isolated splenocytes but greatly
increased by the ex vivo culture for 24 hours as seen in
infected peripheral blood T-cells from HTLV-1 carrier.
As the gene expression of CD25 was also activated in
proportion to the tax induction, function of Tax was
indicated to be responsible for the CD25 expression.
When CD4+ T-cells from HTLV-1 infected spleno-
cytes were separated into CD25+ and CD25- cells, how-
ever, tax expression was observed mainly in CD25- CD4
+ T-cells. These results indicate that the expression of
CD25 may not necessarily involve the Tax function in
vivo. We will discuss the function of Tax in CD25- CD4
+ T-cells and a significance of CD25 expression without
the action of Tax in vivo.
Published: 6 June 2011
doi:10.1186/1742-4690-8-S1-A14
Cite this article as: Tezuka et al.: Inverse correlation between Tax and
CD25 expressions in HTLV-1 infected CD4 T-cells in vivo. Retrovirology
2011 8(Suppl 1):A14.
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* Correspondence: fujisawa@takii.kmu.ac.jp
Dept. Microbiology, Kansai Medical University, Moriguchi, Osaka, 570-8506,
Japan
Tezuka et al. Retrovirology 2011, 8(Suppl 1):A14
http://www.retrovirology.com/content/8/S1/A14
© 2011 Tezuka et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


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Inverse correlation between Tax and CD25 expressions in HTLV-1 infected CD4 T-cells in vivo

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