Alais, Sandrine

Bazerbachi, Ali

Cachat, Anne

de Thé, Hughes

Duc Dodon, Madeleine

El Hajj, Hiba

Gazzolo, Louis

Hermine, Olivier

Villaudy, Julien

Zaatari, Ghazi

Retrovirology

English

PubMed

Julien Villaudy

Anne Cachat

Sandrine Alais

Hiba El Hajj

Louis Gazzolo

Ghazi Zaatari

Olivier Hermine

Hughes de Thé

Ali Bazerbachi

Madeleine Duc Dodon

Springer - Publisher Connector

MEETING ABSTRACT Open Access
HTLV-1-infected HIS Rag2-/-gc-/- mice, a suitable
model for in vivo investigating the effects of
drugs in ATL treatment?
Julien Villaudy1, Anne Cachat1, Sandrine Alais1, Hiba El Hajj2, Louis Gazzolo1, Ghazi Zaatari3, Olivier Hermine4,
Hughes de Thé5, Ali Bazerbachi2, Madeleine Duc Dodon1*
From 15th International Conference on Human Retroviruses: HTLV and Related Viruses
Leuven and Gembloux, Belgium. 5-8 June 2011
Adult T cell Leukemia, an aggressive T-cell malignancy
linked to HTLV-1 infection, is resistant to chemother-
apy. Recently, promising results were obtained with the
combination of arsenic trioxide, interferon alpha and
zidovudine. However, the cellular and molecular
mechanisms of their anti-leukemia activity remain to be
investigated. To that aim, we have relied on HIS
(Human Immune System) Rag2-/-gc-/- mice. We have
indeed observed that when infected with HTLV-1, these
mice displayed, five months later, an elevated number of
human Tax-expressing T cells in the thymus, the spleen
and the mesenteric lymph nodes. Some of them also
developed T lymphoproliferative diseases.
To determine the effects of these three drugs on the
apparition of these pathological features, Rag2-/-gc-/-
mice were infected with HTLV-1 and, 16 weeks after
infection, daily treated with the drugs for one week and
sacrificed. Untreated HIS mice infected with HTLV-1
were used as control. Treatment resulted in a significant
decrease in the spleen weight as compared to untreated
controls. Interestingly, we also noted a decrease of the
proviral load in thymocytes as well as a drop in the
number of mature activated T cells in the spleen and in
lymph nodes. These data clearly indicate that the HIS
mouse model provides us with the opportunity not only
for the pre-clinical evaluation of therapeutic approaches
against the leukemogenic process associated with
HTLV-1 infection, but also for unraveling the corre-
sponding mechanisms.
Author details
1Virologie Humaine, INSERM U758, Ecole Normale Supérieure de Lyon, IFR
128 BioSciences Lyon-Gerland, Lyon, 69364 Cedex 07, France. 2Department
of Internal Medicine, American University of Beirut, Beirut, Lebanon.
3Department of Pathology and laboratory Medicine, American University of
Beirut, Beirut, Lebanon. 4CNRS UMR8603, Hôpital Necker, Paris, 75743 Cedex
15, France. 5Université Diderot-Paris, INSERM U728, Hôpital Saint-Louis, Paris,
75475 Cedex 10, France.
Published: 6 June 2011
doi:10.1186/1742-4690-8-S1-A11
Cite this article as: Villaudy et al.: HTLV-1-infected HIS Rag2-/-gc-/- mice,
a suitable model for in vivo investigating the effects of drugs in ATL
treatment? Retrovirology 2011 8(Suppl 1):A11.
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* Correspondence: mducdodo@ens-lyon.fr
1Virologie Humaine, INSERM U758, Ecole Normale Supérieure de Lyon, IFR
128 BioSciences Lyon-Gerland, Lyon, 69364 Cedex 07, France
Full list of author information is available at the end of the article
Villaudy et al. Retrovirology 2011, 8(Suppl 1):A11
http://www.retrovirology.com/content/8/S1/A11
© 2011 Villaudy et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


HTLV-1-infected HIS Rag2-/-γc-/- mice, a suitable model for in vivo investigating the effects of drugs in ATL treatment?

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HTLV-1-infected HIS Rag2-/-γc-/- mice, a suitable model for in vivo investigating the effects of drugs in ATL treatment?

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