Bangham, Charles R M

Cavallari, Ilaria

Ciminale, Vincenzo

Corradin, Alberto

D'Agostino, Donna M

Jacobson, Steven

Rende, Francesca

Silic-Benussi, Micol

Tanaka, Yuetsu

Taylor, Graham P

Toffolo, Gianna M

Toulza, Frederic

English

PubMed

Francesca Rende

Ilaria Cavallari

Alberto Corradin

Micol Silic-Benussi

Frederic Toulza

Gianna M Toffolo

Yuetsu Tanaka

Steven Jacobson

Graham P Taylor

Donna M D'Agostino

Charles R M Bangham

Vincenzo Ciminale

Springer - Publisher Connector

Kinetics and intracellular compartmentalization of HTLV-1 gene expression

Crossref

MEETING ABSTRACT Open Access
Kinetics and intracellular compartmentalization of
HTLV-1 gene expression
Francesca Rende1,2, Ilaria Cavallari1, Alberto Corradin3, Micol Silic-Benussi1, Frederic Toulza4, Gianna M Toffolo3,
Yuetsu Tanaka5, Steven Jacobson6, Graham P Taylor4, Donna M D’Agostino1, Charles R M Bangham4,
Vincenzo Ciminale1,2*
From 15th International Conference on Human Retroviruses: HTLV and Related Viruses
Leuven and Gembloux, Belgium. 5-8 June 2011
Human T cell leukemia virus Type 1 (HTLV-1) codes for
at least 9 alternatively-spliced transcripts and two major
regulatory proteins named Tax and Rex that function at
the transcriptional and post-transcriptional levels, respec-
tively. We investigated the temporal sequence of HTLV-1
gene expression in primary cells from infected patients
using splice site-specific quantitative RT-PCR. The results
indicated a two-phase kinetics with the Tax/Rex mRNA
preceding expression of other viral transcripts. Using
transfection of HTLV-1 molecular clones and subcellular
RNA fractionation we demonstrated the Rex-dependency
of the two-phase kinetics and determined the compart-
mentalization of the individual mRNAs, showing that over
90% of the HBZ mRNAs were retained in the nucleus.
Mathematical modelling revealed the importance of a
functional delay of Rex function compared to Tax, which
was supported by experimental evidence of delayed accu-
mulation and longer half-life of Rex. These data provided
the first evidence for a temporal pattern of HTLV-1
expression and revealed major differences in the intracel-
lular compartmentalization of HTLV-1 transcripts.
Author details
1Department of Oncology and Surgical Sciences, University of Padova,
Padova, I-35128, Italy. 2Istituto OncologicoVeneto–IRCCS, Padova, Italy.
3Department of Information Engineering, University of Padova, Padova, Italy.
4Department of Immunology, Imperial College, London, UK. 5Department of
Immunology, Graduate School of Medicine, University of the Ryukyus,
Okinawa, Japan. 6Viral Immunology Section, Neuroimmunology Branch,
National Institutes of Health, Bethesda, MD, USA.
Published: 6 June 2011
doi:10.1186/1742-4690-8-S1-A204
Cite this article as: Rende et al.: Kinetics and intracellular
compartmentalization of HTLV-1 gene expression. Retrovirology 2011 8
(Suppl 1):A204.
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* Correspondence: v.ciminale@unipd.it
1Department of Oncology and Surgical Sciences, University of Padova,
Padova, I-35128, Italy
Full list of author information is available at the end of the article
Rende et al. Retrovirology 2011, 8(Suppl 1):A204
http://www.retrovirology.com/content/8/S1/A204
© 2011 Rende et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.


D'Agostino Donna M

Taylor Graham P

Jacobson Steven

Tanaka Yuetsu

Toffolo Gianna M

Toulza Frederic

Silic-Benussi Micol

Corradin Alberto

Cavallari Ilaria

Rende Francesca

Bangham Charles R M

Ciminale Vincenzo

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Kinetics and intracellular compartmentalization of HTLV-1 gene expression

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