Braunersreuther, Vincent

Crowe, Lindsey A

Delattre, Bénédicte MA

Gardier, Stephany

Hyacinthe, Jean-Noël

Mach, François

Vallée, Jean-Paul

English

PubMed

Bénédicte MA Delattre

Vincent Braunersreuther

Stephany Gardier

Jean-Noël Hyacinthe

Lindsey A Crowe

François Mach

Jean-Paul Vallée

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Manganese kinetics demonstrated a double
contrast in acute but not in chronic infarction in
a mouse model of myocardial ischemia
reperfusion
Bénédicte MA Delattre1*, Vincent Braunersreuther2, Stephany Gardier1, Jean-Noël Hyacinthe1, Lindsey A Crowe1,
François Mach2, Jean-Paul Vallée1
From 2011 SCMR/Euro CMR Joint Scientific Sessions
Nice, France. 3-6 February 2011
In this study we investigated whether Manganese (Mn2+)
wash-in kinetics can add new information regarding a
myocardial infarct characterization in particular if it can
differentiate an acute infarct from a chronic scar.
Manganese (Mn2+) is considered as a specific MRI
contrast agent that enters viable cardiomyocytes through
calcium pathways. Compared to extracellular Gadoli-
nium based contrast agents, it has the potential to assess
cell viability and has already shown its ability to accu-
rately measure infarct size with late enhancement ima-
ging. So far, only information from the wash out phase
after recirculation has been used for the detection and
characterization of myocardial infarct.
In this study, we used a model of 60min ischemia
followed by reperfusion on C57/BL6 mice. MRI exam
was performed on a clinical 3T scanner, either 24 hours
(n = 10) or 8 days (n =12) after reperfusion (acute and
chronic infarct respectively). Mn2+-induced signal inten-
sity (SI) kinetics were measured into three distinct areas,
remote, infarction and left ventricular blood pool and
compared to ex vivo TTC and Masson’s trichrome.
For both infarction types (acute and chronic) the SI
kinetics showed a fast entry of the contrast agent into
the blood pool followed by a slower wash-out corre-
sponding to the recirculation phase of Mn2+. The SI
into the remote area increased slowly from the start of
the Mn2+ infusion to still 45 minutes later when a pla-
teau was reached. We observed fast entry of Mn2+ into
acute infarction followed by a recirculation phase that
lead to a double contrast between infarction and
“remote”, whereas the entry of Mn2+ into chronic infarc-
tion was slow and SI stayed lower than in “remote”
(see figure 1). As a main hypothesis, extracellular space
is largely enhanced in acute infarction due to cell mem-
brane rupture (necrosis) and interstitial edema, whereas
scar tissue is densely composed of collagen fibers that
reduce the distribution volume of free Mn2+ ions.
In addition to its ability to depict accurately the
infarcted area at late enhancement, Mn2+ is also able to
discriminate acute versus chronic injury by the observa-
tion of double-contrast wash-in kinetics in a mouse
model of ischemia reperfusion.
Author details
1University of Geneva, Geneva University Hospital, Geneva, Switzerland.
2Geneva University Hospital, Geneva, Switzerland.
Published: 2 February 2011
1University of Geneva, Geneva University Hospital, Geneva, Switzerland
Full list of author information is available at the end of the article
Figure 1 Time course of normalized SI after MnCl2 infusion in
different areas of the myocardium (remote, infarct and left
ventricular blood pool), for mice with acute (left) or chronic (right)
infarction. Bold lines are mean SI and thin lines are SEM.
Delattre et al. Journal of Cardiovascular Magnetic Resonance 2011, 13(Suppl 1):P164
http://jcmr-online.com/content/13/S1/P164
© 2011 Delattre et al; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
doi:10.1186/1532-429X-13-S1-P164
Cite this article as: Delattre et al.: Manganese kinetics demonstrated a
double contrast in acute but not in chronic infarction in a mouse
model of myocardial ischemia reperfusion. Journal of Cardiovascular
Magnetic Resonance 2011 13(Suppl 1):P164.
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Delattre et al. Journal of Cardiovascular Magnetic Resonance 2011, 13(Suppl 1):P164
http://jcmr-online.com/content/13/S1/P164
Page 2 of 2


Manganese kinetics demonstrated a double contrast in acute but not in chronic infarction in a mouse model of myocardial ischemia reperfusion

Mach François

Crowe Lindsey A

Hyacinthe Jean-Noël

Gardier Stephany

Braunersreuther Vincent

Delattre Bénédicte MA

Vallée Jean-Paul

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Manganese kinetics demonstrated a double contrast in acute but not in chronic infarction in a mouse model of myocardial ischemia reperfusion

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