Hao Yu

Lei Fang

Li Yen C

Lu Liu

Xiaojing Yan

Xingliang Hou

Y Lee

PLoS ONE

PubMed

BACKGROUND: The conserved SNF1/AMPK/SnRK1 complexes are global regulators of metabolic responses in eukaryotes and play a key role in the control of energy balance. Although α-type subunits of the SnRK1 complex have been characterized in several plant species, the biological function of β-type and γ-type subunits remains largely unknown. Here, we characterized AtPV42a and AtPV42b, the two homologous genes in Arabidopsis, which encode cystathionine-β-synthase (CBS) domain-containing proteins that belong to the PV42 class of γ-type subunits of the plant SnRK1 complexes. METHODOLOGY/PRINCIPAL FINDINGS: Real-time polymerase chain reaction was performed to examine the expression of AtPV42a and AtPV42b in various tissues. Transgenic plants that expressed artificial microRNAs targeting these two genes were created. Reproductive organ development and fertilization in these plants were examined by various approaches, including histological analysis, scanning electron microscopy, transmission electron microscopy, and phenotypic analyses of reciprocal crosses between wild-type and transgenic plants. We found that AtPV42a and AtPV42b were expressed in various tissues during different developmental stages. Transgenic plants where AtPV42a and AtPV42b were simultaneously silenced developed shorter siliques and reduced seed sets. Such low fertility phenotype resulted from deregulation of late stamen development and impairment of pollen tube attraction conferred by the female gametophyte. CONCLUSIONS: Our results demonstrate that AtPV42a and AtPV42b play redundant roles in regulating male gametogenesis and pollen tube guidance, indicating that the Arabidopsis SnRK1 complexes might be involved in the control of reproductive development

Li Yen Candy Lee

Directory of Open Access Journals

AtPV42a and AtPV42b redundantly regulate reproductive development in Arabidopsis thaliana.

Crossref

AtPV42a and AtPV42b Redundantly Regulate Reproductive Development in Arabidopsis thaliana

, which encode cystathionine-β-synthase (CBS) domain-containing proteins that belong to the PV42 class of γ-type subunits of the plant SnRK1 complexes. were simultaneously silenced developed shorter siliques and reduced seed sets. Such low fertility phenotype resulted from deregulation of late stamen development and impairment of pollen tube attraction conferred by the female gametophyte. SnRK1 complexes might be involved in the control of reproductive development

Fang Lei

Hou Xingliang

Lee Li Yen Candy

Liu Lu

Yan Xiaojing

Yu Hao

Public Library of Science (PLOS)

a crucial step in promoting flower development.

A yeast gene that is essential for release from glucose repression encodes a protein-kinase.

AKIN beta gamma contributes to SnRK1 heterotrimeric complexes and interacts with two proteins implicated in plant pathogen resistance through its KIS/GBD sequence.

AMPK: A key sensor of fuel and energy status in skeletal muscle.

Anther development - basic principles and practical applications.

Arabidopsis thaliana proteins related to the yeast SIP and SNF4 interact with AKIN alpha 1, an SNF1-like protein kinase.

Bateman domains and adenosine derivatives form a binding contract.

Carbon metabolite sensing and signalling.

CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations.

CBS domains: structure, function, and pathology in human proteins.

Characterization of tobacco proteinkinase NPK5, a homolog of Saccharomyces-cerevisiae SNF1 that constitutively activates expression of the glucose-repressible SUC2 gene for a secreted invertase of S. cerevisiae.

Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein-kinase Cdna.

Cyclic AMP-dependent protein kinase regulates the subcellular localization of Snf1-Sip1 protein kinase.

Development and function of the synergid cell.

DOH1, a class 1 knox gene, is required for maintenance of the basic plant architecture and floral transition in orchid.

Domain fusion between SNF1-related kinase subunits during plant evolution.

Double fertilization -caught in the act.

Experimental analysis of the fertilization process.

Expression of antisense SnRK1 protein kinase sequence causes abnormal pollen development and male sterility in transgenic barley.

Floral dip: a simplified method for Agrobacteriummediated transformation of Arabidopsis thaliana.

Functional diversity of potato SNF1-related kinases tested in Saccharomyces cerevisiae.

Functional identification of an Arabidopsis Snf4 ortholog by screening for heterologous multicopy suppressors of snf4 deficiency in yeast.

Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis.

Genome-wide expression profiling of the Arabidopsis female gametophyte identifies families of small, secreted proteins.

Highly specific gene silencing by artificial microRNAs in Arabidopsis.

Identification of SnIP1, a novel protein that interacts with SNF1-related protein kinase (SnRK1). Plant Mol

is essential for angiosperm fertilization.

Male gametophyte development.

Mg2+-sensing mechanism of Mg2+ transporter MgtE probed by molecular dynamics study.

Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants.

Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein.

MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis.

MYB98 positively regulates a battery of synergid-expressed genes encoding filiform apparatus-localized proteins.

Pollen development and fertilization in Arabidopsis is dependent on the MALE GAMETOGENESIS IMPAIRED ANTHERS gene encoding a type VP-type ATPase.

Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases.

Rice has two distinct classes of protein kinase genes related to SNF1 of Saccharomyces cerevisiae, which are differently regulated in early seed development.

Roles of 5 9-AMP-activated protein kinase (AMPK) in mammalian glucose homoeostasis.

Snf1-related protein kinase 1 is needed for growth in a normal day-night light cycle.

SNF1-related protein kinases: global regulators of carbon metabolism in plants?

SNF1/AMPK/SnRK1 kinases, global regulators at the heart of energy control?

Specification of Arabidopsis floral meristem identity by repression of flowering time genes.

Structure and expression of the SNF1 gene of saccharomyces-cerevisiae.

Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism.

Sugar sensing and signaling in plants: Conserved and novel mechanisms.

The AMP-activated protein kinase cascade - a unifying system for energy control.

The AMP-activated protein kinase pathway - new players upstream and downstream.

The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex.

The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of rice.

The structure of a domain common to archaebacteria and the homocystinuria disease protein.

http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.291.6527

AtPV42a and AtPV42b redundantly regulate reproductive development in arabidopsis thaliana

AtPV42a and AtPV42b redundantly regulate reproductive development in arabidopsis thaliana

Abstract

Similar works

Full text

Available Versions

Directory of Open Access Journals

Crossref

Public Library of Science (PLOS)

Public Library of Science (PLOS)