2005-2006 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Wong, MS

Wu, CF

Xie, F

Yao, XS

Zhang, Y

PolyU Institutional Repository

???? ,??????? ( ODC)、 PKC、????、???????? ,?????????????????????????????。References:[1 ]　 Menon L G, Kut tan R, Kut tan G, et al . Inhibi ti on of lungmetastasis in mice induced by B16F10 melanoma cells bypolyphenolic compounds [ J ]. Cancer Let t , 1995, 55 ( 1-2 ):221-225.[2 ]　 Shen X M, Huang W , Huang J Q. Ef fects of curcumin onbiological behaviors of a high metas tatic h uman lung cancersubline [ J]. J Guangdong Med Coll (??????? ) ,2002, 19( 1): 1-3.[3 ]　 Kut tan R, Bhanumath y P, Nirmala K, et al . Potent ial an ti-cancer activi ty of turmeric ( Curcuma lon ga ) [ J] . CancerLet t , 1985, 29( 2): 197-202.[4 ]　 Yuan S J, Han R. The chemoprophylaxis funct ion of cur-cumin again st cancer [ J] . Foreign Med Sci: Cancer Sect (????:????? ) , 1997, 24( 5): 264-268.[5 ]　 Lin J K, Pan M H, Lin-shiau S Y. Recen t s tudies on th ebiofunct ions and biot ransformations of curcumin [ J] . Biofa c-tors , 2000, 13( 1-4): 153-158.[6 ]　 Rash od H, Ling Q, Stw en J S. Curcumin, a natu ral plantph endic food addit ive, inhibi t s al l prolif eration and inducescell cycle changes in colon ad enocarcinoma cel l lines by ap ros taglandin-independent pathw ay [ J] . Lab Med , 1997,130( 6): 576-584.[7 ]　 Koru tla L, Cheung J Y, M endels oh n J, et al . Inh ibi tion ofligand-induced activation of epidermal g row th factor receptortyrosin e phosphorylation by curcumin [ J] . Ca rcinogenesis ,1995, 16( 8): 1741-1745.[8 ]　 Khafi f A, Sch an tz S P, Chou T C, et al . Quan ti tation ofch em op reventive s ynergi sm betw een epigallocatechin-3-gal-late and curcumin in normal , p remaligant and malignant h u-man oral epi th elial cel ls [ J] . Carcinogenesi s, 1998, 19( 3):419-424.[9 ]　 Zhang Y J, Xia T, Zhao J B. Ef fects of mat rine on th e dif-ferentiation of SMM C-7721 cel l line [ J]. J Fou rth Mil MedUniv (???????? ) , 1998, 19( 3): 340-343.[10 ]　 Lin J K, Shi C A. Inhibi tory ef fect of cu rcumin on xanthinedehydrogenas e /oxidase ind uced by phorbol-12-myri state-13-acetate in N IH3T3 cells [ J] . Carcinogenesis , 1994, 15 ( 8):1717-1721.???????????????????????解　芳 1, 3 ,张　岩 1, 3 ,吴春福 1 ,姚新生 2 ,黄文秀 3, 4 ( 1. ????????? ??? ,?? ??　 110016; 2. ?????????? ??????? ,?? ??　 110016;3. ?????????????? ,?? ??　 518057; 4. ?????? ??????????? ,?? ?? )?　?:??　????????????????????。??　? 3?? SD?????????? , 1??? ig????? 17β-??? ( 2 mg /kg )??????????? ( 110 mg /kg ) ,???? 3??。???????????????? ( s-Ca )、? ( s-P)???????? ( ALP) ??????? ( u-Ca)、? ( u-P )???( Cr )??。?? ELISA??????????? ( DPD )??。?????????????????????????。??　??????? ,????? s-Ca、 s-P???? , ALP???? , u-Ca?????? ,?? DPD? Cr??? ( DPD /Cr )???? ,????。?????????? ,???????。 ig???????????? 3?? ,?? ALP???? , u-Ca????? ,?? DPD /Cr?? ,????? ,????????。?????????? ,??????????????????。 ??　??????????????????????????? ,???????????? ,??????????????????? ,??????????????。???: ??? ; ???? ; ??????????: R285. 5　　　?????: A　　　????: 0253 2670( 2005) 11 1667 04Inhibitory effect of Herba Epimedii extract on bone turn-over of ovariectomized ratsX IE Fang1, 3 , ZHANG Yan1, 3 , WU Chun-fu1 , Y AO Xin-sheng2 , WONG Man-sau3, 4( 1. Depar tment o f Pharmaco lo g y, Shenyang Pharmaceutical Univ er sity , Shenyang 110016, China; 2. Depar tment ofNatur al Products Chemistr y, Shenyang Pha rmaceutical Unive rsity , Shenyang 110016, China; 3. Sta te KeyLabo rato ry of Chinese Medicine and Mo lecula r Pha rmaco lo gy , Shenzhen 518057, China; 4. Depa rtment o fApplied Biolog y and Chemica l Technolog y , the Hong Kong Po lytechnic Univ ersity , Hong Kong SAR)Abstract: Objective　 To investiga te the ef fect of Herba Epimedii ex t ract on bone turn-over inovariectomized ra ts. Methods　 Three-month old fema le SD rats w ere ovariectomized and Sham-operated·1667·???　 Chinese T raditional and Herbal D rug s　? 36?? 11? 2005? 11? ????: 2005-03-15????:?????????????????? ( AOE /P-10 /01)????:?　? ( 1977— ) ,? ,??????? ,????????????????? ,?????????????? ,???????????????????????。Tel: ( 0755) 26737176　 Fax: ( 0755) 26972852　 E-mail: jiefangzhi@ yahoo. comrespectiv ely. One month af ter ova riectomy ( OVX) , the rats w ere giv en Herba Epimedii ex t ract ( 110 mg /kg ) and 17β -est rogen ( 2 mg /kg ) by ig fo r three months continuously. Serum contents of Ca ( s-Ca) , P ( s-P) , and ALP as w ell as urine contents o f Ca ( u-Ca) , P ( u-P) , and creatinine ( Cr ) w ere detected. U rineDPD /Cr w as analyzed by ELISA ki t. The proximal tibia wa s processed undecalcified sections o f 5μm fo rhistomorphometric analy sis. Results　 There w as no change among al l groups in the lev els o f s-Ca、 s-P,and u-P. Serum ALP activi ty signi ficant ly increased in OVX rats wi th metrat rophia compared wi th Shamg roup, as w el l as u-Ca and DPD /Cr. OVX g roup had low er t rabecular bone mass wi th decreased a rea per-centag e o f t rabecular bone mass and increased suturae of t rabecular bone mass. Bo th Herba Epimedii ex-tract and 17β -est rog en treatment could make serum ALP activi ty inhibi t , u-Ca and DPD /Cr reduce, t rabe-cula r bone mass increase, and suturae o f t rabecular bone mass const rict in tibia widen. Hysterauxesis w asfound in 17β -est rog en g roup, while no change w as observ ed in Herba Epimedii ex t ract g roup compa redw ith model g roup. Conclusion　 Herba Epimedii ex t ract could inhibit the high ra te of bone turn-overinduced by OVX in rats, wi thout substantial effects on the uterus.Key words: Herba Epimedii ; o steopo rosis; ov ariectomy ( OVX)　　?????????????? [1 ] ,???????? ,?????? [ 2] ,????????????????????????。???? ,?????????????? [ 3]????????? [ 4]?????。 ???????????????????????????????????? [1, 5 ]。?????????????????? ,???????????????????????? ,???????????????????。1　?????1. 1　??: 3?? SD?? 35? ,?? ,?? 190～230 g ,???????????????。1. 2　??????: ???????????Epimedium sagit tatum ( Sieb. et Zucc. ) Maxim.????? ,????? , 5???????? 3? ,????? ,?????????????? ,???? 10%。 ??《????》 ( 2000?? )???????????????????? ( 9. 6±0. 3)% 。1. 3　??:?? Abbo t t Labo ra to ries?? ALCY-ON○R 300i??????? ,?? Bio— Rad550???? ,??? TECN IPLAST ??????? ,????? Olympus A H— 2??????。1. 4　?????: 17β -??? , Sigma?? ;?、???? ,????? ,?????????? (?????????????? ) ;????? ELISA??? ,?? Quidel Co rpo ra tion;???????????。2　??2. 1　??:?? 3???? SD?? 35? ,???? 4?:???? ( 8? )、??? ( 8? )、????( 9? )、???? ( 10? )。2. 2　??:??????? ,??????? , 75%???? ,?????????? ,?????????? ,????? ,???????????? ;????????????????? ,???? ,????????????。2. 3　??:??????????。????????????? 10 g??? ,?????????????????????? ,???????????????。?????? ig?? 110 mg /kg?????????? ,???? ig?? 2 mg /kg?????? ,??????? ig??????。2. 4　????: ?????????? (??22～ 25℃ ) ,?? 4～ 5??????? ,??????????????? 3???? ,???????? ,???? 90 d。2. 5　????2. 5. 1　????????: ????? 1 d,?????????? 24 h?? ,????。? ELISA?????????? ( DPD) ?? ,?????????????? ( u-Ca )、? ( u-P) ???( Cr) ??。2. 5. 2　????????:???????? ,?????? ,?? ,???? ,?????????????? ( s-Ca)、? ( s-P) ????????( ALP)???。2. 5. 3　??: ??? ,??????? ,????? ,????????。2. 5. 4　??????????: ????? ,??·1668· ???　 Chinese T raditional and Herbal D rug s　? 36?? 11? 2005? 11???????? ,????????????????????? ,???? -?????????? ,?????? ,????????????????。??????????? 5μm??? ,?????? ,?????。 ?????? OlympusA H— 2?????? ,?? Leica QW 550???? ,?????? 1 mm???? 4 mm??????? ,???????????????? [6 ]。2. 6　????: ??? x± s?? ,??????????????? t?? ,?????????One-w ay ANOVA?? LSD?? ,?????SPSS10. 0????。3　??3. 1　???????????:?? 1。???????????? ,?????? 2?? ,????????? ,?????????。?????????? ( 110 mg /kg )????????????????。3. 2　???????????:?? 1。??????? ,??????????? (P < 0. 01) ,?????? ,?????????????? ( P <0. 01) ,??????????。? 1　???????????????????? (x± s)Table 1　 Ef fect of Herba Epimedii extract on body weight and uterus of OVX rats (x± s)?　? ?　? /( mg· kg- 1 )?? /??? / g??? ?? 1?? ?? 3???????? /( mg· g- 1)??? - 8 217± 3 261± 7 301± 11 1. 87± 0. 13?? - 8 226± 2 285± 4* * 333± 8* * 0. 32± 0. 01* *??? 　 2 10 227± 4 290± 7* * 295± 8# # 1. 18± 0. 05# #?????? 110 9 229± 5 282± 5* * 342± 11* * 0. 31± 0. 01* *　　　???????: * * P < 0. 01; 　??????: # # P < 0. 01　　　* * P < 0. 01 vs Sham group; 　# # P < 0. 01 vs model g roup3. 3　??????? s-Ca、 s-P、 u-Ca? u-P???:?? 2。??????? s-Ca、 s-P、 u-P??????? ( P> 0. 05) ;??????? ,??????u-Ca??????? ( P < 0. 05) ,?????????????? ,?????? u-Ca??? (P <0. 01)。3. 4　???????? ALP????? DPD?Cr?? ( DPD /Cr )???:?? 2。???? ,??????? ALP????? DPD /Cr????????? (P < 0. 05) ;???????????????? ALP?? ,???????????? ,?????? ALP?????????。?????????????? DPD /Cr???? ( P <0. 05) ,??????。? 2　????????????????????????? ( x± s )Table 2　 Effect of Herba Epimedii extract on serum and urine biochemical parameters of OVX rats (x± s )?　? ?? /( mg· kg- 1 )?? /?s-Ca /( mg· L- 1)s-P /( mg· L- 1)u-Ca /( mg· mg- 1)u-P /( mg· mg- 1 )ALP /( U· L- 1)? DPD /Cr( mmol· mg- 1)??? - 8 11. 0± 0. 2 3. 18± 0. 11 0. 81± 0. 13 1. 82± 0. 17 74. 0± 10. 5 4. 9± 0. 6?? - 8 10. 4± 0. 1 3. 01± 0. 08 1. 35± 0. 15* 1. 68± 0. 08 101. 6± 9. 8* 8. 7± 0. 8*??? 　 2 10 10. 8± 0. 4 3. 13± 0. 16 0. 51± 0. 13# # 2. 07± 0. 35 48. 8± 2. 7# # 6. 3± 1. 1#?????? 110 9 10. 7± 0. 2 2. 98± 0. 23 0. 45± 0. 06# # 1. 83± 0. 07 79. 2± 6. 9# 5. 9± 0. 9#　　　???????: * P < 0. 05; 　??????: # P < 0. 05　# # P < 0. 01　　　* P < 0. 05 vs Sham g roup; 　# P < 0. 05　# # P < 0. 01 vs model group3. 5　?????????????????: ??3。???? ,??????? ,????????????? 67% (P < 0. 01) ,??????????? ,????????? 217. 2% 。?????????????????????????????????? (P < 0. 05) (?? )。4　??　　????????????????????????。 ??? , 70?????????????????? 30% ?? , 50～ 80? ? 26% ?????? ,? 80?? 40% ???????????????。????????????????????。???? ,??????????? ,??????? ,????? ,???????????? ,???????? ,?????? ,????????????????? ,????????????????????。 ?????????? ALP???? , u-Ca????? ,?? DPD?·1669·???　 Chinese T raditional and Herbal D rug s　? 36?? 11? 2005? 11?? 3　????????????????????????? (x± s )Table 3　 Ef fect of Herba Epimedii extract on tibia histo-morphometric parameters of OVX rats (x± s )?　??? /( mg· kg- 1)?? /????????? /%????? /μm????? /μm??? - 8 40. 8± 10. 1 229. 7± 28. 8 55. 8± 4. 0?? - 8 13. 8± 8. 2* * 728. 5± 199. 5* * 55. 3± 6. 5??? 　 2 10 21. 0± 6. 1# 297. 9± 58. 5# 59. 8± 4. 1?????? 110 9 20. 9± 5. 8# 336. 6± 41. 1# 56. 6± 8. 3　　???????: * * P < 0. 01; 　??????: # P < 0. 05　　* * P < 0. 01 vs Sham group; 　# P < 0. 05 vs model g rou p? ,???????????? [7 ]。???????????????????????????? ,????????????????????????。 ?????? ,?????? s-Ca、 s-P???? , ALP????。? u-Ca?????? ,???? DPD???? ,????????????????????????????。?????4?? ,???????????????????。???????????? ,????? ,???????????? ,???????????? ,?????????。 ?????? ig?????? ( 110 mg /kg ) 3?? ,?? ALP?????? , u-Ca??? DPD???? ,???????????。????????????????????????????? ,???????????? ,???????????????????。???????? ,??????????????????????。??? ,?????????????“??”、“??”?? ,????????。???????????????????????? [8 ]、???????? [4 ]??????????? [ 9]????? ,????????? ,??????? -6( IL-6)??。 ??????????? ,???????????????????? [ 10] ,???????????????????? [11 ]。 ??? ,?、?、?????????????????????????????????????? [12, 13 ] ,????????????????? ,??????????。?????????????????????? ,?????????????????? ,??????????? ,?????????????????、?????????????。 ????????????????????????????? ,?????????????。 ????????????????????????????????? ,??????????????????????? ,??????????。References:[1 ]　 An S J. Ef fect of kidn ey-toni fying h erb s on ovary functionand bone mass in pos tmenopausal w omen [ J]. Chin J Osteo-porosis (???????? ) , 2000, 6( 2): 55-59.[2 ]　 Wu H, Lien E J, Lien L L. Chemical and pharmacologicalinvest igation s of Epimedium species: a su rvey [ J] . ProgDrug Res , 2003, 60: 1-57.[3 ]　 Yu S. 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Inhibitory effect of Herba Epimedii extract on bone turn-over of ovariectomized rats

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