The aim of this study was to evaluate the influence of Lactobacillus plantarum isolated and associated with prebiotics (inulin, oligofrutose, β-glucan and polydextrose) on the aflatoxin B1 (AFB1) reduction in artificially contaminated milk.info:eu-repo/semantics/publishedVersio

Becker-Algeri, T.A.

Colla, Eliane

Drunkler, Deisy A.

Moreira, M.C.

Rodrigues, Paula

Vasconcelos, R.A.M.

Wochner, K.F.

English

Wochner, Katia F.

Becker-Algeri, Tania Aparecida

Biblioteca Digital do IPB

INTRODUCTION VASCONCELOS, R.A.M1,2; WOCHNER, K.F. 1; BECKER-ALGERI, T.A. 3; MOREIRA, M.C.C1; RODRIGUES, P.C.A. 2; COLLA, E. 1; DRUNKLER, D.A.1 1. UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ (UTFPR – Câmpus Medianeira, Avenida Brasil, 4232, CEP 85884-000, Medianeira- PR, Brasil); 2. INSTITUTO POLITÉCNICO DE BRAGANÇA (IPB - Campus Santa Apolónia, 5300-253 Bragança, Portugal); 3. UNIVERSIDADE FEDERAL DO RIO GRANDE (FURG) (Avenida Itália Km 8 – Rio Grande – RS, Brasil. CEP: 96201-900). E-mail: deisydrunkler@utfpr.edu.br  EFFECT OF Lactobacillus plantarum AND PREBIOTICS IN REDUCTION OF AFLATOXIN B1 IN MILK  Objective: The aim of this study was to evaluate the influence of Lactobacillus plantarum isolated and associated with prebiotics (inulin, oligofrutose, β-glucan and polydextrose) on the aflatoxin B1 (AFB1) reduction in artificially contaminated milk. Aflatoxin B1 How to remove milk descontamination? 1º PREBIOTIC 3º PROBIOTIC 2º AFB1  Whole cow’s milk MATERIAL AND METHODS % 𝐑𝐑𝐑𝐑𝐑𝐑𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮 = 𝐄𝐄𝐄𝐄𝐄𝐄𝐑𝐑𝐮𝐮𝐮𝐮𝐑𝐑𝐑𝐑 𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐑𝐑𝐮𝐮𝐮𝐮𝐜𝐜𝐜𝐜𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮  𝛍𝛍𝛍𝛍 − 𝐑𝐑𝐑𝐑𝐜𝐜𝐑𝐑 𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐑𝐑𝐮𝐮𝐮𝐮𝐜𝐜𝐜𝐜𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮(𝛍𝛍𝛍𝛍) × 𝟏𝟏𝟏𝟏𝟏𝟏𝐑𝐑𝐑𝐑𝐜𝐜𝐑𝐑 𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐑𝐑𝐮𝐮𝐮𝐮𝐜𝐜𝐜𝐜𝐮𝐮𝐮𝐮𝐮𝐮𝐮𝐮 (𝛍𝛍𝛍𝛍)  RESULTS AND CONCLUSIONS Table 1 – Fourteen-trial Plackett & Burman design matrix for independent variables AFB1 concentration, time, inulin, oligofructose, beta-gluican and polidextrose and dependen variable percentual reduction of AFB1 in whole cow’s milk.        Trial Concentratrion of AFB1 (𝞵𝞵g.L-1) Time (h) Inulin (%) Oligofructose (%) Beta-glucan (%) Polidextrose(%) Reduction (%) 1 +1 (10) -1 (0) + 1 (0,75) - 1 (0) - 1 (0) - 1 (0) 7,57 ± 1,56 2 +1 (10) +1 (6) -1 (0) + 1 (0,75) - 1 (0) - 1 (0) 22,98 ± 0,60 3 -1 (5) +1 (6) + 1 (0,75) - 1 (0) + 1 (0,75) - 1 (0) < LOQ 4 +1 (10) -1 (0) + 1 (0,75) + 1 (0,75) - 1 (0) + 1 (0,75) < LOQ 5 +1 (10) +1 (6) -1 (0) + 1 (0,75) + 1 (0,75) - 1 (0) 0,02 ± 2,52 6 +1 (10) +1 (6) + 1 (0,75) - 1 (0) + 1 (0,75) + 1 (0,75) 30,18 ± 1,97 7 -1 (5) +1 (6) + 1 (0,75) + 1 (0,75) - 1 (0) + 1 (0,75) < LOQ 8 -1 (5) -1 (0) + 1 (0,75) + 1 (0,75) + 1 (0,75) - 1 (0) 55,85 ± 0,66 9 -1(5) -1 (0) -1 (0) + 1 (0,75) + 1 (0,75) + 1 (0,75) 5,48 ± 0,41 10 +1 (10) -1 (0) -1 (0) + 1 (0) + 1 (0,75) + 1 (0,75) 25,43 ± 3,42 11 -1 (5) +1 (6) -1 (0) - 1 (0) - 1 (0) + 1 (0,75) < LOQ 12 -1 (5) -1 (0) -1 (0) - 1 (0) - 1 (0) - 1 (0) 31,45 ± 2,94 13 0 (7,5) 0 (3) 0 (0,38) 0 (0,38) 0 (0,38) 0 (0,38) 25,64 ± 1,54 14 0 (7,5) 0 (3) 0 (0,38) 0 (0,38) 0 (0,38) 0 (0,38) 21,71 ± 2,57 CT1 (7,5) (3) (0) (0) (0) (0) - CT2 (0) (3) (0) (0) (0) (0) ND CT3 (0) (3) (0) (0) (0) (0) -  AFB1 concentration, time of incubation (37ºC)  and prebiotic’s concentration: Table 1.   Concentration of Lactobacillus plantarum: 108 UFC/mL (all trials).   CT1 = positive control – milk + AFB1, CT2 = milk + probiotic; CT3 = milk   Quantification of AFB1: UHPLC-FLD (Dionex Corporation, UltiMate 3000, Sunnyvale, Estados Unidos) .  Aflatoxin B1  Lactobacillus plantarum isolate or in combination with prebiotics has a potential to reduce aflatoxin B1 concentrations in whole cow’s milk. REFERENCES EL-KHOURY, A.; ATOUI, A.; YAGHI, J. Analysis of aflatoxin M1 in milk and yogurt and AFM1 reduction by lactic acid bacteria used in Lebanese industry. Food Control, v.22, n.10, p.1695-1699, 2011. SARTORI, A. V.; MATOS, J. S.; MORAES, M. H. P.; N´OBREGA, A. W. Determination of aflatoxins M1, M2, B1, B2, G1, and G2 and ochratoxin A in UHT and powdered milk by modified QuEChERS method and ultra-high-performance liquid chromatography tandemMass spectrometry. Food Analytical Methods, v. 8, p. 2321–30, 2015. SCAGLIONI, P.; BECKER-ALGERI T.; DRUNKLER, D.; BADIALE-FURLONG, E. Aflatoxin B1 and M1 in milk. Analytica Chimica Acta, v.829, p.68-74, 2014. IMAGENS - https://www.google.com.br/search?q=PROBIOTICS&dcr=0&source=lnms&tbm=isch&sa=X&ved=0ahUKEwiExMz9qv3WAhXBiZAKHXMHC6AQ_AUIDCgD&biw=1440&bih=794  

Effect of Lactobacillus plantarum and prebiotics in reduction of aflatoxin B1 in milk

https://bibliotecadigital.ipb.pt/bitstream/10198/21399/1/2017_29CBM_Vasconcelos.pdf

Effect of Lactobacillus plantarum and prebiotics in reduction of aflatoxin B1 in milk

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