Medicinal plants have been playing a vital role on human health and healing, representing one
of the major sources of drugs in modem and traditional medicine [1]. Plants synthesize and
preserve a variety of biochemical products that can be used as pharmaceutical compounds [2],
and recently there has been an increasing interest in the therapeutic potential of plants as
antioxidants and anti-inflammatories [3]. Oxidative stress and inflammation play critical roles in
the pathogenesis of many diseases, such as cancer, cardiovascular disease, arthritis and
obesity [4], among others. Thus, the aim of this study was to explore the bioactivity of red, white
and pink globe amaranth (different cultivars of Gomphrena globosa L.) hydromethanolic
extracts, namely the antioxidant and anti-inflammatory activities. The antioxidant activity was
tested through radicals scavenging capacity, reducing power, and lipid peroxidation inhibition,
whereas the anti-inflammatory activity was assessed by monitoring the inhibition of nitric oxide
(NO) release in the mouse macrophage-like cell line RAW 264.7. The absence of toxicity of the
extracts was also confirmed by the sulphorodamine B (SRB) assay applied to a porcine liver
primary culture (PLP2) established by the authors.
Among the three studied samples, pink globe amaranth showed the highest antioxidant activity,
with the lowest ECso values (0.25 to 1.02 mg/mL), followed by red (0.41 to 1.30 mg/mL) and
white (0.57 to 1.47 mg/mL) globe amaranth. Regarding the anti-inflammatory activity, pink and
red globe amaranth also revealed the lowest ECso values (133 and 136 pg/mL, respectively),
with white globe amaranth revealing an ECso value of 198pg/mL. None of the extracts presented
cytotoxicity in PLP2 cells up to 400 pg/mL. From the results obtained, we can conclude that the
extracts of these plants can be considered good sources of antioxidants and can also be used
for anti-inflammatory purposes
