Andrade, P.B.

Ferreres, F.

Guerra, Luís

Pereira, J.A.

Seabra, R.M.

Sousa, C.

Valentão, P.

Sousa, Carla

Valentão, Patrícia

Ferreres, Frederico

Seabra, Rosa M.

Andrade, Paula B.

Biblioteca Digital do IPB

First Iberic Meeting on Medicinal Chemistry Anticancer Agents Régua, Douro, Portugal . April 28 - May 1 2007 http://conventio.whee.pt/1immc Program and Abstracts P1 Reactive nitrogen species scavenging by tronchuda cabbage Carla Sousa', Luís Guerra', Patrícia Valentão', Federico Ferreres', José A. Pereira', Rosa M. Seabra', Paula B. Andrade' REQUIMTEI Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Parlo, R. Anibal ;unha, 164, 4050-047 Parlo, Porlugal; b Research Group on Qua/ity, Safety and Bioaclivily of Planl 'oods, Dept. Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus Univ. :spinardo, (Murcla) Spain; c CIMO / Escola Superior Agrária, Inslituto Politécnico de Bragança, :ampus Sla Apolónia, Apartado 1 172, 5301-855 Bragança, Portugal. deimosfobos 1@hotmall.com rhe role 01 reactive nltrogen species (RNS), such as nitric oxide (NO) and peroxynitrile (ONOO·), in ;everal physiological processes has been well recognized, which inelude blood pressure control, leural signal transduction, platelel lunction, and antimicrobial delence. ,., Many palhophysiological )rocesses, inc1uding reperfusion or ischemic tiSSU9. acute Inflammation and sepsis, might initiate 3vents Ihat stimulate NO produclion.' Nilric oxide is produced in various cell Iypes by nitric oxide ;ynlhases and reacts rapidly wilh superoxide lo lorm peroxynitrile. An overproducllon 01 Ihese RNS is 3ssocialed wilh several types 01 biological damage. Delelerious effects include lipids peroxidalion, prolein oxidation and nilration, enzymes Inactivation and DNA damage,'· 5 which lead lo chronic inflammation, cardiovascular diseases, Parkinson's and Alzheimer's diseases and several types of cancer.6-' Scavengers 01 RNS, especially Ihose lrom exogenous sources, may play a pivolal role in prevenling/conlrolling degenerative diseases. Antioxidanls musl reacl wilh reaclive spedes lasler Ihan biologlcal subslrales, Ihus prolecting biological largels lrom oxidative damage. The ability 01 processed Ironchuda cabbage (Brassica oleracea L. var. coslala De) lo acl as a scavenger 01 NO and ONOO· was investigated in in vitro syslems. Nilric oxide was produced by sodium nitroprusside and lhe scavenging ability was accessed by measuring nitrite accumulalion. Peroxynitrile was synlhesized ln lhe reaclion 01 nitrile wilh acidlfled hydrogen peroxide, lollowed by a base quench. The scavenglng capacity was accomplished by monitoring the lormalion of 3-nitrolyrosine. The aqueous extracts oblained Irom lronchuda cabbage seeds and Irom its exlernal and inlernal leaves exhibiled a concenlration dependenl scavenging capaclly (Figure 1). The anlioxidanl polenlial observed agalnst the twa reactive species was as follows: seeds > externa I leaves > internal leaves. ln order to establish a possible correlation with the chemical composition of lhe extracls lhe activily 01 ascorbic and sinaplc acids and kaempferol 3-0-rutinoside was also sludied. Among lhe compounds lesled sinapic acid showed the slrongest antioxldant activily agalnsl bolh specles. ~ 7J ; ~. JO ~ o " " __ h: ...... L ..... , __ lIn d. • , .. ~ 7) ! ~ JD • g u o C ... , .. nulo. (lC .. II) fil,"{"'''''Io. fi" -.) I) Figure 1. Effect oftronchuda cabbage extracts agalnst (A) nitric oxide and ~I:j, perexynllrile. Va lues shaw mean + SE frem 3 experiments performed in triplicale. References 1 Beckman, J.S., Beckman, T.W .. Chen, J., Marshall, P.A., Freeman. B.A. , Proe. Natl, Aead, Sei., 1990, 87,1620. 2 Ford, P.C., Wink, DA , Stanbury. O.M .. FEBS Letl., 1993, 326, 1. 'Zou, Y., Kim, A.R., Kim, J.E., Choi , J.S., Chung, H.Y., J. Ag';c. Food Chem., 2002, 50. 5884. 4 Aruoma, 0.1., Whileman, M., England. T.G., Halllwell. B., Biochem. Blophys. Res. Commun., 1997, 231. 389. 5 Saklhama, Y., Tamaki, R., Shlmojla, H., Ichiba, T., Fukushi, Y., Tahara, S., Yamasaki, H .. FEBS Letl .. 2003. 553,377. ' Vãiinãnen, A.J., Kankuri , E., Rauhala, P., Free Radlc. Biol. Med., 2005 , 38, 1102. 1 Yen, G.-C., lal, H.-H., Food Chem. Toxicol .. 2002, 40. 1433. 'Oixil. K., Moinuddin, Ali, A., Lile Scl. , 2005, 77,2626. 11MMC 2007 59 

Reactive nitrogen species scavenging by tronchuda cabbage.

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Reactive nitrogen species scavenging by tronchuda cabbage.

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