The antibiotics residual presence in honey is a current problem with negative implications, mainly commercial since according to European legislation antibiotic occurrence in honey samples is forbidden

Correia, Daniela M.

Dias, L.G.

Vilas-Boas, Miguel

Biblioteca Digital do IPB

pffi#çtrgffixsé#s #F r${ffsE(OND EUROPEAFI C(}FIFERËFI(E SIAPtt'0t06v 2CI0610 - 14 September 2ü16Prague, Czech Republicedited by Madimír Veself and Dalibor Titëra*aa Euqa Cufuw é,tte@. prWE f*h _ t:Eh sJfsút ZW- Tbe global ph*"E gatüem of honey coütafus proto.at€quic, p-hydroxibenzric,caËeic, chlorogenic'YaniliÊ,trqmÍic, elagic, arucinamic acidsasirln a"*i1g#"k"*rp".fl,-apigenl+ pLrocenurin aüd €dst flavonoids. using mdti;ia& anabísrt iti1 rosg-ible ihe boney dissimimtion of ikee r"rtugret -eËtÇrs Associations byphecrolic pattern.Evanuertox ôF ÁIyïrBroïrcs REsrDïtEs tË'sEr,s Eü poRïucrrEsE ãoNEy: AcoNcuRïrD srrrDy wïrg tm ponrueuess BEDr.'E'ËRS ASSOCTÌr.roì*sDaniela Cnrreiq Mguel yíIas&ou, Luìs G- DiasCIMOIESAB' Caapus SaÍb Apolfuia, Âpartado t tA.5í)l-854 Eragãnçá, porargal E-maih ldia@ipb,ptThe antibioties residuat pr€s"oce in boaey is a cu,,çnt probrem wiú negativeimplications, rminly connercial siace according to ErrpáJ bgblation" antibioticoccumence in honeysampla is foúidda Iu purugal, ttere Ë a goúrg concern amongBeekeepem with the residus of rüese prducb ã ír"*y *dló are committed toprodugmg 1d sellÍng acleanhealth ana mtnratp,otuctIn orderto ovacome this concauç &e PorCIgaese Naaiocal Beekeepers Federation,the Beekeepers,âssociations and Bragança's agãti-, superior s"no"ip".up"t* io ãproject u&ich airm to trace antibioücs in rhe ãaney and soay the di*erent ways ofhoney contamination E Õ"r: resjlues {proj""t gnLceo by tie pornrguese NationalApiculüral PÍogram)- The min ob.iectivJis o tace rhe anhlbiotic residues to nationallevel in order úo infer the global siantio*r of the Porbrguese troney and to promote itsqualrty; the second objective meets &e necessiries oï a. ;;"É.e* of-identifyingsources of honey contarniration-The Beekeryers Âss&iatioas sard a number ofsamples proportional to the volumeg{4":t,p*cuction, wrth-preference beire ervea to ae commeÁa honey with its orrnlabel Different classs of anübiotics i" ú;y were amtlzed such as srlphonamides,t€&ac-yclines- and Strepfomyein The saeenúgs were done b}, CIìARM II and úçpositive resulb were colfirmed by HPLC with ffrnresceÍrce or IFV detector- rn the caseof the positive results' inquires o'rere s€nt to the Be*eep Associatior so thd a $rveycoytd be caÍid out' with the apicuttue invotved, for evalmtiou of the contaminationorigfu.' . - -The resutts shorp tlut clearly there are ao residues of streptomycin, whilefciracplines w"'e fs'od in onty orË sample- rne surptonamüw ae the main concernbut, as the results arr low, üey reflect extà* or prwious years contaminations.tr6Evaluation of Antibiotics Residues Levels in Portuguese Honey:A Concerted Study with the Portuguese Beekeepers AssociationsDaniela M. Coneia. Miguel Vilas-Boas, Luís G. DiasCIMOIESAB, Campus Santa Apolónia, Apartado 1172, 5301-854 Bragança, PortugalVrwW.eSa.tÃtb.pt E-mail: ldías@ipb.p!lntroductionln Portugal, there is a growing concern among Bêekeepers with the antibiotic residues in honey since they are commifted to producing and selling a clean health andnatural product. ln order to overcome this concern, the Portuguese National Beekeepers Federation, the Beekeepers Associations and Bragança's Agrarian SuperiorSchool participate in a project which aims lo tÍace antibiotics in honey and study the different ways of honey contamination by these residues.Objectives'The main objective is to trace the antibiotic residues to national level in orderto infer the global situation of thê Portuguese honey and to promote its quality;'The second objeclive meets up with the necessities of the beekeeper ofidentifying sources of honey conlamination.ResultsFigure 2 -ïhe antibiotics contamination percentage:otfo100,080,060,0,o,020,0Figure í - PortugueseBeekeepers AssociationsL*rul.--'----_ l:]]:J fM{riln II aPrMrL L :i' I I| "-- r-Èitar I I IMNMIllraueu lïl stç-çufbÌa ,-,- E].---f^"ElbcgõiI MPNSE I[pNHMdI \ r- '-- ÌI zeene !_ .1-ò,a.-**,ffi;;;;fMRl LeFB - -_l-HNUsEqlMe-I-r-" 1 1dr*Ì fÁruGffi]f*rc-l ,"b* êc3èT' I aPrcon Il.^r-l-**, -t;j" lE-**tFHm-T-Suifo.âmides ÌêftacycliF3 st.êplohycinsAntibioticsFigure 3 - The inquiry results:Environmeffil sumundingwâbÍ trcaherú sysbms Elí%OtheÉ âpbÍÈs not contrc;led-----ft!wm<:wle*,u liflãõffi]IrcMEL-lBeehavê suNèyNucbus inlÍoductbn o%Behive intrcduclion o%FÉmeshnsÍcr -44%Swams introduclion f----- - _ 33%Beehive handnngComÍcblwax substilrÍion f-33%Wax substitdion - l100olBehive alinentationotheÍ aliBenbtion l-11%Honey oÍ âpbÍbs f-----.-22Y.AÍíthl alineffation -133%VeterinaÍy teahêntsAftibkticsAcaÍÈidesOtlÈ6Honêy eÍÊction condilionshdivklual enÊctionContinuous ettËclionConclusions' No residues of streptomycins detected;o Only two samples with tetracyclines contamination were found;. 24% oÍ honey with sulfonamides contamination.Sulfonamides are the main concern but, since the results are low, they refleclextêmal or previous years contaminations.NEXT STEP:' Analyse the environmental contribution' Study antibiotic residual stability on the beehiveAclmowledgemeÍ*s - Portugues Beekeepers National Fede€iion ':'7 -:.. --t+. 7 - I Finaeial suppoÍt: PoÍtuguese National Apicultural Program-*;. _\r .r*"-_@| *tcrnee | | urrcmar jMaterials and methodsSampling - This initial study involved 155 honey samples obtained from25 Portugal Beekeepers Associations:'The number of samples was proportional to the volume of honeyproduction;' Preference was given to the commercial honey with its own label.Antibiotic analysis anâlysis:'Different classes of antibiotics in honey were analyzed such assulfonamides, tetracyclines and streptomycin.'The screenings were done by CHARM ll.Number of analysis:' SulÍonamides - I 19 honey samples. Tetraciclines - 155 honey samples. Streplomycins - 49 honey samplesPositive results:lnquires to the Beekeeper Association so that a survey could be carried out,with the beekeeper involved, for evaluation of the contamination origin.lnquire:Environmental surrounding, beehive survey, beehive handling, beehivealimêntation, veterinary treatments and honey extraction conditions.

Evaluation of antibiotics residues levels in Portuguese honey: a concerted study with the Portuguese Beekeepers Associations

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Evaluation of antibiotics residues levels in Portuguese honey: a concerted study with the Portuguese Beekeepers Associations

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