Costa López, Gloria

Universitat Autònoma de Barcelona. Facultat de Veterinària

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Prevalence of Pneumocystis carinii in pet rat Pneumocystis organisms are a group of fungi that infect the lung alveoli of mammals, including humans and rats. In an immunosuppressed host, Pneumocystis carinii proliferate in the lung alveoli, causing a lethal pneumonia. The complete life cycle of these organisms has not been fully characterized, primarily due to a historical lack of a long-term culture system, although sexual and asexual stages have been described. Animal studies have shown that transmission occurs through an airborne route. In most humans , the less affected by HIV or other immune causes immunosuppressive individual own ends infection and carriers are few ; but in rats , immunity is usually associated with latent infection, it is important because it is increasing the purchase of pet rats. It’s important to know if it is present actually.  Introduction Gloria Costa López      June, 2016 Objectives Determine the prevalence of Pneumocystis carinii in pet rat through genome detection by PCR techniques (Polymerase Chain Reaction) from oral swabs   Results Material and Methods Acknowledgements Prevalence : 0,0000 % Conclusions Subjects Oral swabs Sampling: Hard palate , the surface and under the  tongue and cheeks Saliva samples DNA analysis Extraction and amplification Revealed Example of real subject with mammary gland fibroadenoma This rat was operated and lived 9 months more. Although very large  mammary gland fibroadenoma manifest, it has not been described to cause immunosuppression PNE-001 Male 2 years Pet Mammary gland fibroadenoma Negative NegativePNE-002 Male 2 years Pet Dilated cardiomyopathy Negative NegativePNE-003 Female 2 years Pet Mammary gland fibroadenoma Negative NegativePNE-004 Female 2 years Pet Osteosarcoma Negative NegativePNE-005 Female 2 years Pet Without pathology Negative NegativePNE-006 Female 2 years Pet Dilated cardiomyopathy Negative NegativePNE-007 Female 2 years Pet Without pathology Negative NegativePNE-008 Male 8 months Pet Without pathology Not performed NegativePNE-009 Female 6 months Pet Mammary gland fibroadenoma Not performed NegativePNE-010 Female 3 months Pet Ovarian cysts Not performed NegativePNE-011 Female 3 years Pet Mammary gland fibroadenoma Negative NegativePNE-012 Male 5 months Pet Without pathology Not performed NegativePNE-013 Female 5 months Pet Without pathology Not performed NegativePNE-014 Male 5 months Pet Without pathology Not performed NegativePNE-015 Female 2 months Pet Without pathology Not performed NegativePNE-016 Female 2 months Pet Without pathology Not performed NegativePNE-017 Male 2 months Pet Without pathology Not performed NegativePNE-018 Male 2 months Pet Without pathology Not performed NegativePNE-019 Male 2 months Pet Without pathology Not performed NegativePNE-020 Male 3 months Laboratory Without pathology Not performed NegativePNE-021 Male 3 months Laboratory Without pathology Not performed NegativePNE-022 Male 3 months Laboratory Without pathology Not performed NegativePNE-023 Male 3 months Laboratory Without pathology Not performed NegativePNE-024 Male 3 months Laboratory Without pathology Not performed NegativePNE-025 Male 3 months Laboratory Without pathology Not performed NegativePNE-026 Male 3 months Laboratory Without pathology Not performed NegativePNE-027 Male 3 months Laboratory Without pathology Not performed NegativePNE-028 Male 3 months Laboratory Without pathology Not performed NegativePNE-029 Male 3 months Laboratory Without pathology Not performed NegativePNE-030 Male 3 months Laboratory Without pathology Not performed NegativePNE-031 Male 3 months Laboratory Without pathology Not performed NegativePNE-032 Male 3 months Laboratory Without pathology Not performed NegativePCR resultsSample code Sex Age Origin Pathology Pulmonary histopathology• The method employed for detecting Pneumocystis carinii from oral swabs, and making a subsequent polymerase chain reaction or PCR is fast and non-invasive . • With a 0 % prevalence of P. carinii can be concluded that there is latent or is currently present in the oral cavity of domestic and laboratory rats . • The presence of some diseases do not seem to influence the expression of P. carinii . since  those disease do not cause immunosuppression, the key to develop a fungal infection. • Parameters such as sex , age and origin does not affect the prevalence of P. carinii.  

Prevalence of Pneumocystis carinii in pet rat

https://ddd.uab.cat/pub/tfg/2016/170258/TFG_gcostalopez_poster.pdf

Prevalence of Pneumocystis carinii in pet rat

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