Brandwein, Christiane

Chourbaji, Sabine

Garner, Joseph P.

Gass, Peter

Lewejohann, Lars

Richter, S. Helene

Sachser, Norbert

Schindler, Britta

Spruijt, Berry

Touma, Chadi

van der Harst, Johanneke

van Stipdonk, Niek

Voikar, Vootele

Wolfer, David P.

Wuerbel, Hanno

Zipser, Benjamin

PLoS ONE

PubMed

S. Helene Richter

Joseph P. Garner

Benjamin Zipser

Lars Lewejohann

Norbert Sachser

Chadi Touma

Britta Schindler

Sabine Chourbaji

Christiane Brandwein

Peter Gass

Niek van Stipdonk

Johanneke van der Harst

Berry Spruijt

Vootele Võikar

David P. Wolfer

Hanno Würbel

Crossref

Effect of Population Heterogenization on the Reproducibility of Mouse Behavior: A Multi-Laboratory Study

Helsingin yliopiston digitaalinen arkisto

Effect of Population Heterogenization on the Reproducibility of Mouse Behavior : A Multi-Laboratory Study

In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the specific experimental conditions. Indeed, we have recently shown in mice that standardization may generate spurious results in behavioral tests, accounting for poor reproducibility, and that this can be avoided by population heterogenization through systematic variation of experimental conditions. Here, we examined whether a simple form of heterogenization effectively improves reproducibility of test results in a multi-laboratory situation. Each of six laboratories independently ordered 64 female mice of two inbred strains (C57BL/6NCrl, DBA/2NCrl) and examined them for strain differences in five commonly used behavioral tests under two different experimental designs. In the standardized design, experimental conditions were standardized as much as possible in each laboratory, while they were systematically varied with respect to the animals' test age and cage enrichment in the heterogenized design. Although heterogenization tended to improve reproducibility by increasing within-experiment variation relative to between-experiment variation, the effect was too weak to account for the large variation between laboratories. However, our findings confirm the potential of systematic heterogenization for improving reproducibility of animal experiments and highlight the need for effective and practicable heterogenization strategies

S Helene Richter

Joseph P Garner

David P Wolfer

Directory of Open Access Journals

Effect of population heterogenization on the reproducibility of mouse behavior: a multi-laboratory study.

In animal experiments, animals, husbandry and test procedures are traditionally standardized to maximize test sensitivity and minimize animal use, assuming that this will also guarantee reproducibility. However, by reducing within-experiment variation, standardization may limit inference to the specific experimental conditions. Indeed, we have recently shown in mice that standardization may generate spurious results in behavioral tests, accounting for poor reproducibility, and that this can be avoided by population heterogenization through systematic variation of experimental conditions. Here, we examined whether a simple form of heterogenization effectively improves reproducibility of test results in a multi-laboratory situation. Each of six laboratories independently ordered 64 female mice of two inbred strains (C57BL/6NCrl, DBA/2NCrl) and examined them for strain differences in five commonly used behavioral tests under two different experimental designs. In the standardized design, experimental conditions were standardized as much as possible in each laboratory, while they were systematically varied with respect to the animals' test age and cage enrichment in the heterogenized design. Although heterogenization tended to improve reproducibility by increasing within-experiment variation relative to between-experiment variation, the effect was too weak to account for the large variation between laboratories. However, our findings confirm the potential of systematic heterogenization for improving reproducibility of animal experiments and highlight the need for effective and practicable heterogenization strategies.ISSN:1932-620

Stipdonk, Niek van

Harst, Johanneke van der

Võikar, Vootele

Wolfer, David Paul

Würbel, Hanno

Repository for Publications and Research Data

Richter, S.

Garner, J.

Zipser, B.

Lewejohann, L.

Sachser, N.

Touma, C.

Schindler, B.

Chourbaji, S.

Brandwein, C.

Gass, P.

van Stipdonk, N.

van der Harst, J.

Spruijt, B.

Vöikar, V.

Wolfer, D.

Würbel, H.

MPG.PuRe

Richter, S. H.

Garner, J. P.

Wolfer, D. P.

Max Planck Society eDoc Server

Wurbel, H

Bern Open Repository and Information System (BORIS)

Richter, S H

Garner, J P

Zipser, B

Lewejohann, L

Sachser, N

Touma, C

Schindler, B

Chourbaji, S

Brandwein, C

Gass, P

van Stipdonk, N

van der Harst, J

Spruijt, B

Võikar, V

Wolfer, D P

Würbel, H

ZORA

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http://boris.unibe.ch/12314/1/journal.pone.0016461.pdf

Effect of Population Heterogenization on the Reproducibility of Mouse Behavior : A Multi-Laboratory Study

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Crossref

Helsingin yliopiston digitaalinen arkisto

Directory of Open Access Journals

Repository for Publications and Research Data

MPG.PuRe

Max Planck Society eDoc Server

Bern Open Repository and Information System (BORIS)

ZORA

MPG.PuRe